Human IL-2 Quantikine HS ELISA Kit

Catalog # Availability Size / Price Qty
HS200
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Human IL-2 ELISA Standard Curve
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Product Details
Citations (6)
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Human IL-2 Quantikine HS ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
0.066 pg/mL
Assay Range
0.3 - 20 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® HS Human IL-2 Immunoassay kit is a 4.0 hour solid phase ELISA designed to measure human IL-2 levels in serum and plasma. It contains
E. coli-expressed recombinant human IL-2 and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-2 accurately. Results obtained using natural human IL-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine® HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 1.89 5.6 11.3 1.89 5.59 11.4
Standard Deviation 0.04 0.13 0.225 0.133 0.327 0.787
CV% 2.1 2.3 2 7 5.8 6.9

Recovery

The recovery of natural human IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 91 84-96
Heparin Plasma (n=4) 88 79-95
Serum (n=4) 91 86-94

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of natural human IL-2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IL-2 ELISA Linearity

Scientific Data

Human IL-2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-2

Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a 15-18 kDa variably glycosylated alpha -helical polypeptide that is a member of the Common gamma Chain ( gamma c) cytokine family (1-4). It exists as a monomer and has a notably short half-life (< 30 minutes) (1). Human IL-2 is synthesized as a 153 amino acid (aa) precursor that contains a 20 aa signal sequence plus a 133 aa mature region (5, 6). The mature region is alpha -helical in nature, and contains one utilized O-linked glycosylation site at Thr3 plus three cysteines, two of which form an intrachain disulfide bond that is essential for activity (7). Mature human IL-2 shares 73%, 66%, 78% and 97% aa identity with canine, rat, feline and rhesus monkey IL-2, respectively. Although human IL-2 shares only approximately 60% aa identity with the highly polymorphic mouse IL-2, human IL-2 is known to be active on mouse IL-2 responsive cells. Cells reported to secrete IL-2 include gamma δ T cells (8), activated conventional CD4+ and CD8+ T cells (1, 9), neurons (10, 11), microglia (12), and hematopoietic stem cells (13). 

The receptor for IL-2 (IL-2 R) is composed of three subunits, the 55 kDa CD25/IL-2 R alpha chain, the 70 kDa IL-2 R beta chain, and the 65 kDa Common gamma Chain (1, 3). IL-2 first binds to CD25, the binary complex then recruits IL-2 R beta and gamma c to form the quaternary signaling complex (1, 14). In addition to IL-2, IL-2 R beta is used by IL-15 in its quaternary signaling complex. gamma c also serves as a signaling receptor for IL-4, -7, -9, -15, and -21 (1, 3). 
In vitro studies have shown an important role for IL-2 in T cell activation and expansion. In vivo, IL-2 is critical for the development, maintenance and function of regulatory T cells (Treg) which provide protection against autoimmune disease. On the other hand, IL-2 can also promote autoimmune inflammation in target organs through its roles in regulating the expression of T cell trafficking genes, and production of Th2 cytokines. Within the CD8+ T cell subset, IL-2 is essential for optimal primary responses and differentiation into terminal effector cells. IL-2 also promotes the development of activated CD8+ T cells into memory cells. (1).

Long Name:
Interleukin 2
Entrez Gene IDs:
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names:
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-2 Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Inflammatory Markers and Risk of Heart Failure With Reduced to Preserved Ejection Fraction
    Authors: Z Albar, M Albakri, J Hajjari, M Karnib, SE Janus, SG Al-Kindi
    The American journal of cardiology, 2022;0(0):.
    Species: Human
    Sample Types: Serum
  2. Comparative evaluation of inflammatory parameters and substitute insulin resistance indices in elderly women with and without type 2 diabetes mellitus
    Authors: MM Leite, MT Dutra, MVG da Costa, SS Funghetto, AO Silva, LR de Lima, ICR da Silva, MR Mota, MM Stival
    Experimental gerontology, 2021;150(0):111389.
    Species: Human
    Sample Types: Plasma
  3. Changes in protein expression due to metformin treatment and hyperinsulinemia in a human endometrial cancer cell line
    Authors: C Lange, A Machado We, R Schmidt, C Schroeder, T Strowitzki, A Germeyer
    PLoS ONE, 2021;16(3):e0248103.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. New signatures of poor CD4 cell recovery after suppressive antiretroviral therapy in HIV-1-infected individuals: involvement of miR-192, IL-6, sCD14 and miR-144
    Authors: F Hernández-, MJ Ruiz-de-Le, I Rosado-Sán, E Vázquez, M Leal, S Moreno, F Vidal, J Blanco, YM Pacheco, A Vallejo
    Sci Rep, 2020;10(1):2937.
    Species: Human
    Sample Types: Plasma
  5. Association between baseline pro-inflammatory cytokines and brain activation during social exclusion in patients with vulnerability to suicide and depressive disorder
    Authors: I Conejero, I Jaussent, A Cazals, E Thouvenot, T Mura, E Le Bars, S Guillaume, S Squalli, P Courtet, E Olié
    Psychoneuroendocrinology, 2019;99(0):236-242.
    Species: Human
    Sample Types: Serum
  6. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
    Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
    Clin. Cancer Res., 2006;12(2):376-82.
    Species: Human
    Sample Types: Plasma

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