Human IL-2 Quantikine HS ELISA Kit

  (1 citations)     
Datasheet / CoA / SDS
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
  • Assay Length
    4.0 hours
  • Sample Type & Volume Required Per Well
    Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    0.066 pg/mL
  • Assay Range
    0.3 - 20 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IL-2.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC238 , Quantikine Immunoassay Control Set 933 for Human HS IL-2 - Please Inquire
Product Summary
The Quantikine® HS Human IL-2 Immunoassay kit is a 4.0 hour solid phase ELISA designed to measure human IL-2 levels in serum and plasma. It contains
E. coli-expressed recombinant human IL-2 and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human IL-2 accurately. Results obtained using natural human IL-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine® HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 1.89 5.6 11.3 1.89 5.59 11.4
Standard Deviation 0.04 0.13 0.225 0.133 0.327 0.787
CV% 2.1 2.3 2 7 5.8 6.9

Recovery

The recovery of natural human IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 91 84-96
Heparin Plasma (n=4) 88 79-95
Serum (n=4) 91 86-94
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of natural human IL-2 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
 IL-2
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-2
IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.
  • Long Name:
    Interleukin 2
  • Entrez Gene IDs:
    3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
  • Alternate Names:
    aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma.
    Authors: Yamanaka K, Clark R, Dowgiert R, Hurwitz D, Shibata M, Rich BE, Hirahara K, Jones DA, Eapen S, Mizutani H, Kupper TS
    Clin. Cancer Res., 2006;12(2):376-82.
    Species: Human
    Sample Type: Plasma

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 933 for Human HS IL-2

Ctrl QC238
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human IL-2 Quantikine HS ELISA Kit?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee