Human IL-22 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM7821

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Product Details
Citations (4)
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Human IL-22 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-22 in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 142928
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-22
Ala34-Ile179
Accession # Q9GZX6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample

Human IL-22 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human IL‑22 Antibody (Catalog # MAB7822R)

Capture Reagent: Human IL‑22 Antibody (Catalog # MAB7822)

Standard: Recombinant Human IL-22 Protein (Catalog # 782-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT‑1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10 R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

References
  1. Dumoutier, L. et al., (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al., (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al., (2000) PNAS 97:10144.
  4. Kotenko, S.V. et al., (2001) J. Biol. Chem. 276:2725.
Long Name
Interleukin 22
Entrez Gene IDs
50616 (Human); 50929 (Mouse); 500836 (Rat)
Alternate Names
Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18

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Citations for Human IL-22 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Expanded IL-22+ Group 3 Innate Lymphoid Cells and Role of Oxidized LDL-C in the Pathogenesis of Axial Spondyloarthritis with Dyslipidaemia
    Authors: Hong Ki Min, Jeonghyeon Moon, Seon-Yeong Lee, A Ram Lee, Chae Rim Lee, Jennifer Lee et al.
    Immune Network
  2. Interleukin (IL)-25 suppresses IL-22-induced osteoclastogenesis in rheumatoid arthritis via STAT3 and p38 MAPK/I&kappaB&alpha pathway
    Authors: HK Min, JY Won, BM Kim, KA Lee, SJ Lee, SH Lee, HR Kim, KW Kim
    Arthritis Research & Therapy, 2020-09-23;22(1):222.
    Species: Human
    Sample Types: Synovial Fluid
  3. Machine-learning algorithms define pathogen-specific local immune fingerprints in peritoneal dialysis patients with bacterial infections
    Authors: J Zhang, IM Friberg, A Kift-Morga, G Parekh, MP Morgan, AR Liuzzi, CY Lin, KL Donovan, CS Colmont, PH Morgan, P Davis, I Weeks, DJ Fraser, N Topley, M Eberl
    Kidney Int, 2017-03-17;0(0):.
    Species: Human
    Sample Types: Peritoneal Effluent
    Applications: ELISA Development (Detection)
  4. IL-22 mediates mucosal host defense against Gram-negative bacterial pneumonia.
    Authors: Aujla SJ, Chan YR, Zheng M, Fei M, Askew DJ, Pociask DA, Reinhart TA, McAllister F, Edeal J, Gaus K, Husain S, Kreindler JL, Dubin PJ, Pilewski JM, Myerburg MM, Mason CA, Iwakura Y, Kolls JK
    Nat. Med., 2008-02-10;14(3):275-81.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development

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