Human IL-22 Antibody (142928)

R&D Systems | Catalog # MAB7821

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Neutralization, Intracellular Staining by Flow Cytometry

Cited:

Immunohistochemistry-Paraffin, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 142928
View all IL-22 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-22
Ala34-Ile179
Accession # Q9GZX6

Specificity

Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL‑22 and recombinant rat IL‑22 and no cross-reactivity with recombinant human IL‑10.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-22 Antibody (142928)

Detection of IL‑22 in PBMC treated with 20ng/mL PMA and 1ug/mL Calcium iono for 2 hours followed by 1ug/mL brefeldin A for 4 hours.

PBMC treated with 20ng/mL PMA and 1ug/mL Calcium iono for 2 hours followed by 1ug/mL brefeldin A for 4 hours were stained with Mouse Anti-Human CD4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3791F) and either (A) Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7821) or (B) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
IL‑10 Secretion Induced by IL‑22 and Neutralization by Human IL-22 Antibody.

IL‑10 Secretion Induced by IL‑22 and Neutralization by Human IL-22 Antibody.

Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adeno-carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-22 Monoclonal Antibody (Catalog # MAB7821). The ND50 is typically 1-5 µg/mL.
Human IL-22 ELISA Standard Curve

Human IL-22 ELISA Standard Curve

Recombinant Human IL‑22 (Catalog # 782-IL) was serially diluted and captured by Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7822) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7821) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human IL-22 Antibody (142928)

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: PBMC treated with 20ng/mL PMA and 1ug/mL calcium iono for 2 hours followed by brefeldin A for 4 hours

Neutralization

Measured by its ability to neutralize IL‑22-induced IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. Nagalakshmi, M.L. et al. (2004) Intl. Immunopharmacol. 4:679. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑22.

Reviewed Applications

Read 1 review rated 5 using MAB7821 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

References

  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Long Name

Interleukin 22

Alternate Names

IL-TIF, IL22

Entrez Gene IDs

50616 (Human); 50929 (Mouse); 500836 (Rat)

Gene Symbol

IL22

UniProt

Q9GZX6

Additional IL-22 Products

Product Documents for Human IL-22 Antibody (142928)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-22 Antibody (142928)

For research use only

Citations for Human IL-22 Antibody (142928)

Customer Reviews for Human IL-22 Antibody (142928) (1)

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  • Human IL-22 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/27/2017
    MAB7821 was used as the solid phase antibody, BAM1359 was used as the detection antibody. 1259-RN was used as the immunoassay standard. Sensitivity of the assay was around 100 pg/ml.
    Human IL-22 Antibody (142928) MAB7821

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