Human IL-22 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of IL‑22 in Human PBMCs stimulated to induce Th17 Cells by Flow Cytometry.
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Product Details
Citations (11)
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Human IL-22 APC-conjugated Antibody Summary

Species Reactivity
Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL‑22 and recombinant rat IL‑22 and no cross-reactivity with recombinant human IL‑10.
Monoclonal Mouse IgG1 Clone # 142928
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human IL-22
Accession # Q9GZX6
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-22 antibody in Human PBMCs stimulated to induce Th17 Cells antibody by Flow Cytometry. View Larger

Detection of IL‑22 in Human PBMCs stimulated to induce Th17 Cells by Flow Cytometry. CD4+Human peripheral blood mononuclear cells (PBMCs) either (A) stimulated to induce Th17 cells with 10 ug/mL Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100), 5 ug/mL Mouse Anti-Human CD28 Monoclonal Antibody (Catalog # MAB342), 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL), 20 ng/mL Recombinant Human IL-23 (Catalog # 1290-IL), 40 ng/mL Recombinant Human IL-6 (Catalog # 206-IL), and 10 ng/mL Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) for 5 to 7 days, then re-stimulated with 50 ng/mL PMA and 200 ng/mL Calcium ionomycin for 2 to 4 hours or (B) resting CD4+PBMCs were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and Mouse Anti-Human IL-22 APC-conjugated Monoclonal Antibody (Catalog # IC7821A). Quadrant markers were set based on isotype control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
Long Name
Interleukin 22
Entrez Gene IDs
50616 (Human); 50929 (Mouse); 500836 (Rat)
Alternate Names
Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18

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Citations for Human IL-22 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Reduced Interleukin-17-Expressing Cells in Cutaneous Melanoma
    Authors: A Tosi, L Nardinocch, ML Carbone, L Capriotti, E Pagani, S Mastroeni, C Fortes, F Scopelliti, C Cattani, F Passarelli, A Rosato, S D'Atri, CM Failla, A Cavani
    Biomedicines, 2021-12-16;9(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Mycobacterium tuberculosis-Specific IL-21+IFN-gamma+CD4+ T Cells Are Regulated by IL-12.
    Authors: Li L, Jiang Y, Lao S, Yang B, Yu S, Zhang Y, Wu C
    PLoS ONE, 2016-01-19;11(1):e0147356.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Trained immunity in newborn infants of HBV-infected mothers.
    Authors: Hong M, Sandalova E, Low D, Gehring A, Fieni S, Amadei B, Urbani S, Chong Y, Guccione E, Bertoletti A
    Nat Commun, 2015-03-25;6(0):6588.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC-Fr
  4. Initiation of ART during early acute HIV infection preserves mucosal Th17 function and reverses HIV-related immune activation.
    Authors: Schuetz A, Deleage C, Sereti I, Rerknimitr R, Phanuphak N, Phuang-Ngern Y, Estes J, Sandler N, Sukhumvittaya S, Marovich M, Jongrakthaitae S, Akapirat S, Fletscher J, Kroon E, Dewar R, Trichavaroj R, Chomchey N, Douek D, O Connell R, Ngauy V, Robb M, Phanuphak P, Michael N, Excler J, Kim J, de Souza M, Ananworanich J
    PLoS Pathog, 2014-12-11;10(12):e1004543.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. CX(3)CL1 (fractalkine) and its receptor CX(3)CR1 regulate atopic dermatitis by controlling effector T cell retention in inflamed skin.
    Authors: Staumont-Salle D, Fleury S, Lazzari A, Molendi-Coste O, Hornez N, Lavogiez C, Kanda A, Wartelle J, Fries A, Pennino D, Mionnet C, Prawitt J, Bouchaert E, Delaporte E, Glaichenhaus N, Staels B, Julia V, Dombrowicz D
    J Exp Med, 2014-05-12;211(6):1185-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. IL-7 licenses activation of human liver intrasinusoidal mucosal-associated invariant T cells.
    Authors: Tang, Xin-Zi, Jo, Juandy, Tan, Anthony, Sandalova, Elena, Chia, Adeline, Tan, Kai Chah, Lee, Kang Hoe, Gehring, Adam J, De Libero, Gennaro, Bertoletti, Antonio
    J Immunol, 2013-02-27;190(7):3142-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Rapid antigen processing and presentation of a protective and immunodominant HLA-B*27-restricted hepatitis C virus-specific CD8+ T-cell epitope.
    Authors: Schmidt J, Iversen A, Tenzer S, Gostick E, Price D, Lohmann V, Distler U, Bowness P, Schild H, Blum H, Klenerman P, Neumann-Haefelin C, Thimme R
    PLoS Pathog, 2012-11-29;8(11):e1003042.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients.
    Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S
    J Allergy Clin Immunol, 2012-11-19;131(2):562-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes.
    Authors: Chan Y, Chen K, Duncan S, Lathrop K, Latoche J, Logar A, Pociask D, Wahlberg B, Ray P, Ray A, Pilewski J, Kolls J
    J Allergy Clin Immunol, 2012-07-11;131(4):1117-29, 1129.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. ESAT-6- and CFP-10-specific Th1, Th22 and Th17 cells in tuberculous pleurisy may contribute to the local immune response against Mycobacterium tuberculosis infection.
    Authors: Qiao D, Yang BY, Li L, Ma JJ, Zhang XL, Lao SH, Wu CY
    Scand. J. Immunol., 2011-04-01;73(4):330-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription.
    Authors: Liu Y, Yang B, Ma J, Wang H, Huang F, Zhang J, Chen H, Wu C
    Immunology, 2011-01-07;132(4):540-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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