Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Neutralization, Flow Cytometry, Bioassay

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 142928
View all IL-22 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-22
Ala34-Ile179
Accession # Q9GZX6

Specificity

Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL‑22 and recombinant rat IL‑22 and no cross-reactivity with recombinant human IL‑10.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL‑22 PE‑conjugated Antibody

Detection of IL‑22 in PBMC treated with 20ng/mL PMA and 1ug/mL Calcium iono for 2 hours followed by 1ug/mL brefeldin A for 4 hours.

PBMC treated with 20ng/mL PMA and 1ug/mL Calcium iono for 2 hours followed by 1ug/mL brefeldin A for 4 hours were stained with Mouse Anti-Human CD4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3791F) and either (A) Mouse Anti-Human IL‑22 PE Monoclonal Antibody (Catalog # IC7821P) or (B) isotype control antibody (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of IL-22 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of IL‑22 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Ca++Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human IL-22 PE-conjugated Monoclonal Antibody (Catalog # IC7821P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human IL‑22 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: PBMC treated with 20ng/mL PMA and 1ug/mL calcium iono for 2 hours followed by 1ug/mL brefeldin A for 4 hours.
Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Ca++ Ionomycin, LPS, and Recombinant Human IL‑23 (Catalog # 1290-IL) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 8 reviews rated 4 using IC7821P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-22

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

References

  1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
  2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Long Name

Interleukin 22

Alternate Names

IL-TIF, IL22

Entrez Gene IDs

50616 (Human); 50929 (Mouse); 500836 (Rat)

Gene Symbol

IL22

UniProt

Q9GZX6

Additional IL-22 Products

Product Documents for Human IL‑22 PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human IL‑22 PE‑conjugated Antibody

For research use only

Citations for Human IL‑22 PE‑conjugated Antibody

Customer Reviews for Human IL‑22 PE‑conjugated Antibody (8)

4 out of 5
8 Customer Ratings
5 Stars
25%
4 Stars
50%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 8 reviews Showing All
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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 22621183
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 22621183
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 21525997
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 21525997
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 21206487
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 21206487
    Species: Human
    Verified Customer | Posted 02/03/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 22070501
    Species: Human
    Verified Customer | Posted 01/28/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 22070501
    Species: Human
    Verified Customer | Posted 01/28/2015

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