Human IL-23 Quantikine ELISA Kit

  (12 citations)
(1 Review)
    
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Assay Procedure
Citations (12)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    16.3 pg/mL
  • Assay Range
    39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IL-23. This assay does not recognize free p19 or p40 subunits.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC23 , Quantikine Immunoassay Control Group 8 - Please Inquire
Product Summary
The Quantikine Human IL-23 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IL-23 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-23 and has been shown to accurately quantitate the recombinant factor. Results obtained for naturally occurring human IL-23 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IL-23.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 180 496 1170 203 526 1298
Standard Deviation 12 23.2 49.6 13.3 45.1 78.5
CV% 6.7 4.7 4.2 6.6 8.6 6

Recovery

The recovery of human IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 87-107
EDTA Plasma (n=4) 95 86-106
Heparin Plasma (n=4) 93 85-99
Serum (n=4) 98 88-105
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-23 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 IL-23A/IL-23 P19 [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-23
IL-23 (Interleukin-23) is a disulfide-linked cytokine composed of a p19 subunit that is unique to IL-23 and a p40 subunit that is shared with IL-12. It is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens. IL-23 induces the earliest recruitment of neutrophils to the site of infection and promotes the development and maintenance of Th17 cells. It also enhances the development of Th17 mediated autoimmunity and tumor progression. IL-23 signals through a receptor complex consisting of IL-12 R beta 1 and IL-23 R. This complex is expressed in mouse Th1 and Th2 cells, bone marrow dendritic cells, activated macrophages and CD4+ CD45Rb(low) memory T cells.
  • Long Name:
    Interleukin 23
  • Entrez Gene IDs:
    51561 (Human); 83430 (Mouse)
  • Alternate Names:
    IL23; IL-23; IL23A; IL-23A; IL-23-A; IL-23p19; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Sample Type
  1. Elevations of novel cytokines in bacterial meningitis in infants
    Authors: L Srinivasan, L Kilpatrick, SS Shah, S Abbasi, MC Harris
    PLoS ONE, 2018;13(2):e0181449.
    Species: Human
    Sample Type: CSF
  2. MMP-8 and TIMP-1 are associated to periodontal inflammation in patients with rheumatoid arthritis under methotrexate immunosuppression - First results of a cross-sectional study
    Authors: G Schmalz, I Davarpanah, J Jäger, RF Mausberg, B Krohn-Grim, J Schmidt, R Haak, U Sack, D Ziebolz
    J Microbiol Immunol Infect, 2017;0(0):.
    Species: Human
    Sample Type: Serum
  3. High serum resistin associates with intrahepatic inflammation and necrosis: an index of disease severity for patients with chronic HBV infection
    Authors: Z Meng, Y Zhang, Z Wei, P Liu, J Kang, Y Zhang, D Ma, C Ke, Y Chen, J Luo, Z Gong
    BMC Gastroenterol, 2017;17(1):6.
    Species: Human
    Sample Type: Serum
  4. Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
    Int J Mol Sci, 2016;17(8):.
    Species: Human
    Sample Type: Serum
  5. Effects of anticipated neonatal surgical intervention on maternal milk cytokine production
    Authors: Jennifer L Liedel
    J. Pediatr. Surg., 2016;0(0):.
    Species: Human
    Sample Type: Milk
  6. Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization
    Authors: Ofer Levy
    J. Immunol., 2016;0(0):.
    Species: Human
    Sample Type: Cell Culture Supenates
  7. Adenoma-linked barrier defects and microbial products drive IL-23/IL-17-mediated tumour growth.
    Authors: Grivennikov S, Wang K, Mucida D, Stewart C, Schnabl B, Jauch D, Taniguchi K, Yu G, Osterreicher C, Hung K, Datz C, Feng Y, Fearon E, Oukka M, Tessarollo L, Coppola V, Yarovinsky F, Cheroutre H, Eckmann L, Trinchieri G, Karin M
    Nature, 2012;491(7423):254-8.
    Species: Human
    Sample Type: Tissue Homogenates
  8. Kinetics of circulating Th17 cytokines and adipokines in psoriasis patients.
    Authors: Nakajima H, Nakajima K, Tarutani M, Morishige R, Sano S
    Arch. Dermatol. Res., 2011;303(6):451-5.
    Species: Human
    Sample Type: Serum
  9. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
    Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
    Leukemia, 2011;25(1):161-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  10. CD14-independent responses induced by a synthetic lipid A mimetic.
    Authors: Legat A, Thomas S, Hermand P, Van Mechelen M, Goldman M, De Wit D
    Eur J Immunol, 2010;40(3):797-802.
    Species: Human
    Sample Type: Cell Culture Supernates
  11. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009;119(10):1424-32.
    Species: Human
    Sample Type: Plasma
  12. Upregulated IL-23 and IL-17 in Behcet patients with active uveitis.
    Authors: Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2008;49(7):3058-64.
    Species: Human
    Sample Type: Serum
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Average Rating: 5 (Based on 1 review)

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 Human IL-23 Quantikine ELISA Kit D2300B Array D2300B
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  Human Anonymous 10/26/2015
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 Human IL-23 Quantikine ELISA Kit D2300B
: Human IL-23 Quantikine ELISA Kit [D2300B].

Summary

Sample Tested Dendritic cells
Species Human

Other Experimental Details

Other Experimental Details Standard curve is shown.
Buffer: kit dilution buffer
Dilution: 1/100

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