Human IL-23 Quantikine ELISA Kit

(7 citations)
(1 Review)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    16.3 pg/mL
  • Assay Range
    39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IL-23. This assay does not recognize free p19 or p40 subunits.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC23, Quantikine Immunoassay Control Group 8 - Please Inquire
Product Summary
The Quantikine Human IL-23 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IL-23 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-23 and has been shown to accurately quantitate the recombinant factor. Results obtained for naturally occurring human IL-23 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IL-23.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation1223.249.613.345.178.5


The recovery of human IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 87-107
EDTA Plasma (n=4) 95 86-106
Heparin Plasma (n=4) 93 85-99
Serum (n=4) 98 88-105
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-23 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-23 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-23
IL-23 (Interleukin-23) is a disulfide-linked cytokine composed of a p19 subunit that is unique to IL-23 and a p40 subunit that is shared with IL-12. It is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens. IL-23 induces the earliest recruitment of neutrophils to the site of infection and promotes the development and maintenance of Th17 cells. It also enhances the development of Th17 mediated autoimmunity and tumor progression. IL-23 signals through a receptor complex consisting of IL-12 R beta 1 and IL-23 R. This complex is expressed in mouse Th1 and Th2 cells, bone marrow dendritic cells, activated macrophages and CD4+ CD45Rb(low) memory T cells.
    • Long Name
      Interleukin 23
    • Entrez Gene IDs
      51561 (Human); 83430 (Mouse);
    • Alternate Names
      IL23; IL-23; IL23A; IL-23A; IL-23-A; IL-23p19; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 7 of 7
    Filter your results:

    Sample Type
    1. Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
      Int J Mol Sci, 2016;17(8):.
      Species: Human
      Sample Type: Serum
    2. Adenoma-linked barrier defects and microbial products drive IL-23/IL-17-mediated tumour growth.
      Authors: Grivennikov S, Wang K, Mucida D, Stewart C, Schnabl B, Jauch D, Taniguchi K, Yu G, Osterreicher C, Hung K, Datz C, Feng Y, Fearon E, Oukka M, Tessarollo L, Coppola V, Yarovinsky F, Cheroutre H, Eckmann L, Trinchieri G, Karin M
      Nature, 2012;491(7423):254-8.
      Species: Human
      Sample Type: Tissue Homogenates
    3. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
      Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
      Leukemia, 2011;25(1):161-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Kinetics of circulating Th17 cytokines and adipokines in psoriasis patients.
      Authors: Nakajima H, Nakajima K, Tarutani M, Morishige R, Sano S
      Arch. Dermatol. Res., 2011;303(6):451-5.
      Species: Human
      Sample Type: Serum
    5. CD14-independent responses induced by a synthetic lipid A mimetic.
      Authors: Legat A, Thomas S, Hermand P, Van Mechelen M, Goldman M, De Wit D
      Eur J Immunol, 2010;40(3):797-802.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
      Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
      Circulation, 2009;119(10):1424-32.
      Species: Human
      Sample Type: Plasma
    7. Upregulated IL-23 and IL-17 in Behcet patients with active uveitis.
      Authors: Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A
      Invest. Ophthalmol. Vis. Sci., 2008;49(7):3058-64.
      Species: Human
      Sample Type: Serum
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Group 8

    Ctrl QC23
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Average Rating: 5 (Based on 1 review)

    Have you used Human IL-23 Quantikine ELISA Kit?

    Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Read what people are saying who have used Human IL-23 Quantikine ELISA Kit.

    We have 1 review tested in 1 species: Human.

    Filter by Ratings
    All Ratings
    Filter by Applications
    All Applications
    Filter by Species
    All Species

    Images Ratings Applications Species Reviewed By Date Details
     Human IL-23 Quantikine ELISA Kit D2300B
      Human Anonymous 10/26/2015
    Image Details
     Human IL-23 Quantikine ELISA Kit D2300B
    : Human IL-23 Quantikine ELISA Kit [D2300B]


    Sample TestedDendritic cells

    Other Experimental Details

    Other Experimental DetailsStandard curve is shown.
    Buffer: kit dilution buffer
    Dilution: 1/100

    Order Details

    Contact Information

    Certificate of Analysis Lookup

    The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

    Certificate of Analysis Lookup

    Research Areas of Interest
    R&D Systems Guarantee

    Customers Who Viewed
    This Item Also Viewed


    Customer Information