Human IL‑28 R alpha /IFN‑ lambda  R1 Antibody

Detection of Human IL‑28 R alpha /IFN‑ lambda R1 by Western Blot.

Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human IL-28 Ra/IFN-lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-28 Ra/IFN-lambda R1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of IL‑28 R alpha /IFN‑ lambda R1 in Daudi Human Cell Line by Flow Cytometry.

Daudi human Burkitt's lymphoma cell line was stained with Human IL-28 Ra/IFN-lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Detection of IL-28 R alpha /IFN-lambda R1 by Western Blot

Type III IFN response regulates HEV replication.(A) Immunoblots of IFNAR1, IFNLR1 and beta -actin in HepG2 cells transduced with lentiviruses expressing gene-specific shRNA or GFP (Ctrl). (B) ISRE promoter activity in different cells after IFN-alpha or IFN-lambda treatment. HepG2 cells were transfected with an interferon-sensitive response element (ISRE) promoter-driven firefly luciferase reporter plasmid ISRE-Luc and a herpes simplex virus thymidine kinase promoter-driven Renilla luciferase (TK-RLuc) plasmid for 24 h, then treated with IFN-alpha (100ng/ml) or IFN-lambda 1 (220ng/ml) for 24 h. Data are expressed as fold changes relative to non-treated cells based on the relative luciferase activities (firefly luciferase vs. Renilla luciferase). Shown are representative results (mean ± SEM) from two independent experiments each performed in triplicate. (C-E) Effects of IFN receptor knockdown on ISG expression and HEV replication. HepG2 cells transduced with lentiviruses expressing gene-specific shRNA or GFP (Ctrl) were infected with HEV for 5 days. (C) Intracellular IFIT1 mRNA expression determined by qRT-PCR. Results are represented as fold changes relative to HEV-infected control cells. (D) HEV RNA abundance determined by qRT-PCR (fold changes relative to HEV-infected control cells). (E) Cells expressing different shRNA or GFP (Ctrl) were infected with HEV for 5 days, harvested and subjected to three rounds of freeze-thaws prior to inoculation to naïve HepG2 cells. Infected cells were detected by IFA and HEV foci were counted after 5 days. Each data point represents the mean ± SEM of at least 2 independent experiments in duplicate each. * P<0.05; ** P<0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28558073), licensed under a CC-BY license. Not internally tested by R&D Systems.