Human IL-28 R alpha /IFN-lambda R1 Antibody Summary
IL‑28 R alpha is observed.
Accession # Q8IU57
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IL‑28 R alpha /IFN‑ lambda R1 by Western Blot. Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human IL-28 Ra/IFN-? R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-28 Ra/IFN-? R1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of IL‑28 R alpha /IFN‑ lambda R1 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Human IL-28 Ra/IFN-? R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-28 R alpha/IFN-lambda R1
IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a type I transmembrane glycoprotein that is the cytokine receptor family 2 member 12
(CRF2-12) (1-4). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R (1-4). Each subunit of this receptor can interact with the
interferon-like cytokines (type III interferons) IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3) or IL-29 (IFN-lambda 1) (1-4). Human IL-28 R alpha cDNA encodes a 520 amino acid (aa) protein with a 20 aa signal peptide, a 208 aa extracellular domain (ECD) with a fibronectin type III motif and four potential N-glycosylation sites, a 21 aa transmembrane sequence, and a proline-rich and acidic 271 aa cytoplasmic domain. Eight isoforms have been sequenced, but their significance is unknown (3, 5). Isoforms of 211 and 244 aa appear to lack transmembrane sequences. These and other isoforms of 491, 437, 322, 283 and 184 aa have alternate N- or C-termini, or lack an internal sequence (aa 268-296). The mature human IL-28 R alpha ECD shares 67%, 66%, 78% and 74% aa sequence identity with mouse, rat, canine and bovine IL-28 R alpha, respectively. Some cross-species reactivity has been shown (6). IL-28 R is constitutively expressed in most tissues, but its ligands are mainly produced by antigen presenting cells in response to viruses and their products (2-6). Signaling through IL-28 R alpha is similar to that of receptors for type I IFNs, including tyrosine phosphorylation, activation of JAK tyrosine kinases, STAT phosphorylation and formation of the IFN-stimulated gene factor 3 (ISGF-3) transcription factor complex
(1-7). This signaling pathway induces antiviral activity and up-regulates MHC class I antigen expression (2-7). Anti-proliferative activity has also been shown for
IL-28/IL-28 R (7).
- Chen, Q. et al. (2006) Vitam. Horm. 74:207.
- Meager, A. et al. (2005) Cytokine 31:109.
- Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
- Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
- Entrez Accession # NP_775088, EAW95113, EAW95115, EAW95116, AAI39723, AAI01409.
- Lasfar, A. et al. (2006) Cancer Res. 66:4468.
- Dumoutier, L. et al. (2004) J. Biol. Chem. 279:32269.
Citations for Human IL-28 R alpha /IFN-lambda R1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Casein kinase 1? mediates degradation of receptors for type I and type II interferons caused by hemagglutinin of influenza A virus
Authors: C Xia, JJ Wolf, M Vijayan, CJ Studstill, W Ma, B Hahm
J. Virol., 2018;0(0):.
Sample Types: Cell Lysates
Applications: Western Blot
A paradox of transcriptional and functional innate interferon responses of human intestinal enteroids to enteric virus infection
Authors: K Saxena, LM Simon, XL Zeng, SE Blutt, SE Crawford, NP Sastri, UC Karandikar, NJ Ajami, NC Zachos, O Kovbasnjuk, M Donowitz, ME Conner, CA Shaw, MK Estes
Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E570-E579.
Species: Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Whole Cells
Type III IFN interleukin-28 mediates the antitumor efficacy of oncolytic virus VSV in immune-competent mouse models of cancer.
Authors: Wongthida P, Diaz RM, Galivo F
Cancer Res., 2010;70(11):4539-49.
Sample Types: In Vivo
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