Human IL-28 R alpha /IFN-lambda R1 Antibody

Catalog #: AF5260
3 Citations Datasheet / COA / SDS

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AF5260
AF5260-SP

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Detection of Human IL‑28 R alpha /IFN‑ lambda R1 by Western Blot.

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-28 R alpha /IFN-lambda R1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-28 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse
IL‑28 R alpha is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1
Arg21-Ala228
Accession # Q8IU57

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL‑28 R alpha /IFN‑ lambda R1 by Western Blot. Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human IL-28 Ra/IFN-lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-28 Ra/IFN-lambda R1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry

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Detection of IL‑28 R alpha /IFN‑ lambda R1 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Human IL-28 Ra/IFN-lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-28 R alpha/IFN-lambda R1

IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a type I transmembrane glycoprotein that is the cytokine receptor family 2 member 12
(CRF2-12) (1-4). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R (1-4). Each subunit of this receptor can interact with the
interferon-like cytokines (type III interferons) IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3) or IL-29 (IFN-lambda 1) (1-4). Human IL-28 R alpha cDNA encodes a 520 amino acid (aa) protein with a 20 aa signal peptide, a 208 aa extracellular domain (ECD) with a fibronectin type III motif and four potential N-glycosylation sites, a 21 aa transmembrane sequence, and a proline-rich and acidic 271 aa cytoplasmic domain. Eight isoforms have been sequenced, but their significance is unknown (3, 5). Isoforms of 211 and 244 aa appear to lack transmembrane sequences. These and other isoforms of 491, 437, 322, 283 and 184 aa have alternate N- or C-termini, or lack an internal sequence (aa 268-296). The mature human IL-28 R alpha ECD shares 67%, 66%, 78% and 74% aa sequence identity with mouse, rat, canine and bovine IL-28 R alpha, respectively. Some cross-species reactivity has been shown (6). IL-28 R is constitutively expressed in most tissues, but its ligands are mainly produced by antigen presenting cells in response to viruses and their products (2-6). Signaling through IL-28 R alpha is similar to that of receptors for type I IFNs, including tyrosine phosphorylation, activation of JAK tyrosine kinases, STAT phosphorylation and formation of the IFN-stimulated gene factor 3 (ISGF-3) transcription factor complex
(1-7). This signaling pathway induces antiviral activity and up-regulates MHC class I antigen expression (2-7). Anti-proliferative activity has also been shown for
IL-28/IL-28 R (7).

References

Chen, Q. et al. (2006) Vitam. Horm. 74:207.
Meager, A. et al. (2005) Cytokine 31:109.
Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
Entrez Accession # NP_775088, EAW95113, EAW95115, EAW95116, AAI39723, AAI01409.
Lasfar, A. et al. (2006) Cancer Res. 66:4468.
Dumoutier, L. et al. (2004) J. Biol. Chem. 279:32269.

Long Name

Interleukin 28 Receptor alpha

Entrez Gene IDs

163702 (Human); 242700 (Mouse); 362628 (Rat)

Alternate Names

class II cytokine receptor CRF2/12; CRF2/12; CRF2-12; Cytokine receptor class-II member 12; Cytokine receptor family 2 member 12; IFN-lambda R1; IFN-lambda receptor 1; IFN-lambda-R1; IFNLR; IFNLR1; IL-28 R alpha; IL-28 receptor subunit alpha; IL28R alpha; IL-28R1; IL28RA; IL-28Ra; IL-28R-alpha; Interferon lambda receptor 1; interferon lambda, receptor 1; interleukin 28 receptor A; interleukin 28 receptor, alpha (interferon, lambda receptor); interleukin 28 receptor, alpha; interleukin or cytokine receptor 2; interleukin-28 receptor subunit alpha; LICR2; Likely interleukin or cytokine receptor 2

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human IL-28 R alpha /IFN-lambda R1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Casein kinase 1? mediates degradation of receptors for type I and type II interferons caused by hemagglutinin of influenza A virus
Authors: C Xia, JJ Wolf, M Vijayan, CJ Studstill, W Ma, B Hahm
J. Virol., 2018-03-14;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
A paradox of transcriptional and functional innate interferon responses of human intestinal enteroids to enteric virus infection
Authors: K Saxena, LM Simon, XL Zeng, SE Blutt, SE Crawford, NP Sastri, UC Karandikar, NJ Ajami, NC Zachos, O Kovbasnjuk, M Donowitz, ME Conner, CA Shaw, MK Estes
Proc. Natl. Acad. Sci. U.S.A, 2017-01-09;114(4):E570-E579.
Species: Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Whole Cells
Applications: Neutralization
Type III IFN interleukin-28 mediates the antitumor efficacy of oncolytic virus VSV in immune-competent mouse models of cancer.
Authors: Wongthida P, Diaz RM, Galivo F
Cancer Res., 2010-05-18;70(11):4539-49.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Neutralization