Human IL-28 R alpha /IFN-lambda  R1 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-28 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse
    IL‑28 R alpha is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1
    Arg21-Ala228
    Accession # Q8IU57
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IL‑28 R alpha /IFN‑ lambda  R1 by Western Blot. Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human IL‑28 R alpha /IFN‑ lambda  R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL‑28 R alpha /IFN‑ lambda  R1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of IL‑28 R alpha /IFN‑ lambda  R1 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Human IL‑28 R alpha /IFN‑ lambda  R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-28 R alpha/IFN-lambda R1

IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a type I transmembrane glycoprotein that is the cytokine receptor family 2 member 12
(CRF2-12) (1-4). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R (1-4). Each subunit of this receptor can interact with the
interferon-like cytokines (type III interferons) IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3) or IL-29 (IFN-lambda 1) (1-4). Human IL-28 R alpha cDNA encodes a 520 amino acid (aa) protein with a 20 aa signal peptide, a 208 aa extracellular domain (ECD) with a fibronectin type III motif and four potential N-glycosylation sites, a 21 aa transmembrane sequence, and a proline-rich and acidic 271 aa cytoplasmic domain. Eight isoforms have been sequenced, but their significance is unknown (3, 5). Isoforms of 211 and 244 aa appear to lack transmembrane sequences. These and other isoforms of 491, 437, 322, 283 and 184 aa have alternate N- or C-termini, or lack an internal sequence (aa 268-296). The mature human IL-28 R alpha ECD shares 67%, 66%, 78% and 74% aa sequence identity with mouse, rat, canine and bovine IL-28 R alpha, respectively. Some cross-species reactivity has been shown (6). IL-28 R is constitutively expressed in most tissues, but its ligands are mainly produced by antigen presenting cells in response to viruses and their products (2-6). Signaling through IL-28 R alpha is similar to that of receptors for type I IFNs, including tyrosine phosphorylation, activation of JAK tyrosine kinases, STAT phosphorylation and formation of the IFN-stimulated gene factor 3 (ISGF-3) transcription factor complex
(1-7). This signaling pathway induces antiviral activity and up-regulates MHC class I antigen expression (2-7). Anti-proliferative activity has also been shown for
IL-28/IL-28 R (7).

  • References:
    1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
    2. Meager, A. et al. (2005) Cytokine 31:109.
    3. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
    4. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
    5. Entrez Accession # NP_775088, EAW95113, EAW95115, EAW95116, AAI39723, AAI01409.
    6. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
    7. Dumoutier, L. et al. (2004) J. Biol. Chem. 279:32269.
  • Long Name:
    Interleukin 28 Receptor alpha
  • Entrez Gene IDs:
    163702 (Human); 242700 (Mouse); 362628 (Rat)
  • Alternate Names:
    class II cytokine receptor CRF2/12; CRF2/12; CRF2-12; Cytokine receptor class-II member 12; Cytokine receptor family 2 member 12; IFN-lambda R1; IFN-lambda receptor 1; IFN-lambda-R1; IFNLR; IFNLR1; IL-28 R alpha; IL-28 receptor subunit alpha; IL28R alpha; IL-28R1; IL28RA; IL-28Ra; IL-28R-alpha; Interferon lambda receptor 1; interferon lambda, receptor 1; interleukin 28 receptor A; interleukin 28 receptor, alpha (interferon, lambda receptor); interleukin 28 receptor, alpha; interleukin or cytokine receptor 2; interleukin-28 receptor subunit alpha; LICR2; Likely interleukin or cytokine receptor 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. A paradox of transcriptional and functional innate interferon responses of human intestinal enteroids to enteric virus infection
    Authors: K Saxena, LM Simon, XL Zeng, SE Blutt, SE Crawford, NP Sastri, UC Karandikar, NJ Ajami, NC Zachos, O Kovbasnjuk, M Donowitz, ME Conner, CA Shaw, MK Estes
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E570-E579.
    Species: Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Type: Whole Cells
    Application: Neut
  2. Type III IFN interleukin-28 mediates the antitumor efficacy of oncolytic virus VSV in immune-competent mouse models of cancer.
    Authors: Wongthida P, Diaz RM, Galivo F
    Cancer Res., 2010;70(11):4539-49.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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