Human IL‑33 Biotinylated Antibody (Catalog #
Recombinant Human IL-33 Protein (Catalog #
Measured by its ability to neutralize IL‑33-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑33 and sub-optimal amounts of Mouse CD3 epsilon Monoclonal Antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑33 and Neutralization by Human IL‑33 Antibody.
In the presence of sub-optimal amounts of Hamster Anti‑Mouse CD3 epsilon Monoclonal Antibody (Catalog # MAB484), Recombinant Human IL‑33 (Catalog # 3625-IL) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human IL‑33 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625). The ND50 is typically 0.75-3.0 µg/mL.
IL‑33 in Human Tonsil.
IL‑33 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is up‑regulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is up‑regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature human IL-33 share 52‑58% aa sequence identity with mouse and rat IL-33. Human IL-33 shares less than 20% aa sequence identity with other IL-1 family proteins.
Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
Schmitz, J. et al. (2005) Immunity 23:479.
Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
Xu, D. et al. (1998) J. Exp. Med. 187:787.
Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
Dinarello, C.A. (2005) Immunity 23:461.
Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
Entrez Gene IDs:
90865 (Human); 77125 (Mouse)
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2
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We have 3 review tested in 1 species: Human.
We have 3 reviews tested in 3 applications: Immunohistochemistry, ELISA, Immunocytochemistry/Immunofluorescence.
Immunohistochemistry: Human IL-33 Antibody [AF3625].
Other Experimental Details
Other Experimental Details
Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287Used at 10ug/ml.Briefly, frozen brain sections were fixed in 4% PFA(Fisher Scientific), followed by antigen retrieval using heating in acidcitric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector).Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc ReceptorBlocking Solution was added (Human TruStain FcX Biolegend,London, UK). Primary antibodies were added overnight at 4 °C.IL-33 was detected with donkey anti-goat-biotin (ab6578,Abcam), followed by streptavidin-HRP and visualised with DAB. Section was counterstained with Hematoxylin.
AF3625 was used as both the capture and the detection antibody for the sandwich ELISA for IL-33. The immunoassay standard was 3625-IL. Assay had sensitivity of ~1pg/ml. Many healthy human samples were not detectable.