Human IL-34 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-34 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse IL-34 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑34
    Asn21-Pro242
    Accession # NP_689669
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IL‑34-induced proliferation in human peripheral blood monocytes. Lin, H. et al. (2008) Science 320:807. The Neutralization Dose (ND50) is typically 0.035-0.175 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑34.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑34 and Neutralization by Human IL‑34 Antibody. Recombinant Human IL‑34 (Catalog # 5265-IL) stimulates proliferation in human peripheral blood monocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑34 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human IL‑34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5265). The ND50 is typically 0.035‑0.175 µg/mL.
Detection of Human IL‑34 by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IL‑34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5265) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL‑34 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

  • References:
    1. Lin, H. et al. (2008) Science 320:807.
  • Long Name:
    Interleukin 34
  • Entrez Gene IDs:
    146433 (Human); 76527 (Mouse)
  • Alternate Names:
    C16orf77; IL34; IL-34
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease.
    Authors: Baek J, Zeng R, Weinmann-Menke J, Valerius M, Wada Y, Ajay A, Colonna M, Kelley V
    J Clin Invest, 2015;125(8):3198-214.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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