Human IL-34 Antibody Summary
Accession # NP_689669
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑34 and Neutralization by Human IL‑34 Antibody. Recombinant Human IL-34 (Catalog # 5265-IL) stimulates proliferation in human peripheral blood monocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-34 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Human IL-34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5265). The ND50 is typically 0.035-0.175 µg/mL.
Detection of Human IL‑34 by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human IL-34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5265) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-34 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).
Citations for Human IL-34 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Inhibition of IL-34 Unveils Tissue-Selectivity and Is Sufficient to Reduce Microglial Proliferation in a Model of Chronic Neurodegeneration.
Authors: Obst J, Simon E, Martin-Estebane M, Pipi E, Barkwill L, Gonzalez-Rivera I, Buchanan F, Prescott A, Faust D, Fox S, Brownlees J, Taylor D, Perry V, Nuthall H, Atkinson P, Karran E, Routledge C, Gomez-Nicola D
Front Immunol, 2020-10-08;11(0):579000.
Sample Types: In Vivo
Applications: In Vivo
IL-34 mediates acute kidney injury and worsens subsequent chronic kidney disease.
Authors: Baek J, Zeng R, Weinmann-Menke J, Valerius M, Wada Y, Ajay A, Colonna M, Kelley V
J Clin Invest, 2015-06-29;125(8):3198-214.
Sample Types: Tissue Homogenates
Applications: Western Blot
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