Human IL-36 gamma /IL-1F9 Antibody

Catalog # Availability Size / Price Qty
MAB2320-SP
MAB2320-500
MAB2320-100
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (2)

Human IL-36 gamma /IL-1F9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑36 gamma /IL‑1F9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-36Ra/IL-1F5, rhIL-36 alpha /IL-1F6, rhIL-1F7, rhIL-36 beta /IL-1F8, rhIL-1ra/IL-1F3, or rhIL-18/IL-1F4 is observed.
Source
Monoclonal Rat IgG2A Clone # 278706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑36 gamma /IL‑1F9
Met1-Asp169
Accession # Q9NZH8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑36 gamma /IL‑1F9 (Catalog # 2320-IL)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.15-0.6 µg/mL of this antibody will block 50% of the binding of 5 µg/mL of Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera to immobilized Recombinant Human IL‑36 gamma /IL‑1F9 coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 gamma/IL-1F9

Interleukin-36 gamma (IL-36 gamma ) [also named IL-1F9, IL-1 epsilon  (epsilon), and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-36 gamma is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293T cells (5). Human to mouse, IL-36 gamma has 53% aa sequence identity. Within the family, IL-36 gamma shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-36 gamma include Langerhans cells, keratinocytes/stratified squamous epithelium, plus chief cells and parietal cells of the stomach (4, 5). The receptor for IL-36 gamma is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-36 gamma, along with IL-36 beta /IL-1F8 and IL-36 alpha /IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

References
  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Debets, R. et al. (2001) J. Immunol. 167:1440.
  6. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 36 gamma/Interleukin 1 Family 9
Entrez Gene IDs
56300 (Human); 215257 (Mouse); 499744 (Rat)
Alternate Names
IL-1 epsilon; IL-1 H1; IL-1 Related Protein 2; IL-1(EPSILON); IL1E; IL-1-epsilon; IL1F9; IL-1F9; IL1H1; IL-1H1; IL-1-Related Protein 2; IL1RP2; IL-1rp2; IL36 gamma; IL-36 gamma; IL36G; interleukin 1 family, member 9; interleukin 1-related protein 2; Interleukin 36, Gamma; Interleukin-1 epsilon; interleukin-1 family member 9; Interleukin-1 homolog 1; Interleukin-36 Gamma

Product Datasheets

Citations for Human IL-36 gamma /IL-1F9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression.
    Authors: Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
    J. Immunol., 2011;186(4):2613-22.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  2. Interleukin-1 family cytokines and their regulatory proteins in normal pregnancy and pre-eclampsia.
    Authors: Southcombe J, Redman C, Sargent I, Granne I
    Clin Exp Immunol, 0;181(3):480-90.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Human IL-36 gamma /IL-1F9 Antibody

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Human IL-36 gamma /IL-1F9 Antibody
By Anonymous on 05/01/2020
Application: B/N Sample Tested: Cell Supernatant Species: Human

Human IL-36 gamma /IL-1F9 Antibody
By Anonymous on 05/01/2020
Application: WB Sample Tested: Cell Lysates Species: Human