Interleukin-36 gamma (IL-36 gamma ) [also named IL-1F9, IL-1 epsilon (epsilon), and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-36 gamma is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293T cells (5). Human to mouse, IL-36 gamma has 53% aa sequence identity. Within the family, IL-36 gamma shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-36 gamma include Langerhans cells, keratinocytes/stratified squamous epithelium, plus chief cells and parietal cells of the stomach (4, 5). The receptor for IL-36 gamma is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-36 gamma, along with IL-36 beta /IL-1F8 and IL-36 alpha /IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.
Human IL‑36 gamma /IL‑1F9 Antibody
R&D Systems | Catalog # MAB2320
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Blockade of Receptor-ligand Interaction
Cited:
Western Blot, ELISA Capture, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 278706
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Product Specifications
Immunogen
E. coli-derived recombinant human IL‑36 gamma /IL‑1F9
Met1-Asp169
Accession # Q9NZH8
Met1-Asp169
Accession # Q9NZH8
Specificity
Detects human IL‑36 gamma /IL‑1F9 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-36Ra/IL-1F5, rhIL-36 alpha /IL-1F6, rhIL-1F7, rhIL-36 beta /IL-1F8, rhIL-1ra/IL-1F3, or rhIL-18/IL-1F4 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human IL‑36 gamma /IL‑1F9 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.15-0.6 µg/mL of this antibody will block 50% of the binding of 5 µg/mL of Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera to immobilized Recombinant Human IL‑36 gamma /IL‑1F9 coated at 1 µg/mL (100 µL/well). At 10 µg/mL, this antibody will block >90% of the binding.
Western Blot
1 µg/mL
Sample: Recombinant Human IL‑36 gamma /IL‑1F9 (Catalog # 2320-IL)
Sample: Recombinant Human IL‑36 gamma /IL‑1F9 (Catalog # 2320-IL)
Reviewed Applications
Read 2 reviews rated 4 using MAB2320 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-36 gamma/IL-1F9
References
- Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
- Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
- Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
- Dunn, E. et al. (2001) Trends Immunol. 22:533.
- Debets, R. et al. (2001) J. Immunol. 167:1440.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 36 gamma/Interleukin 1 Family 9
Alternate Names
IL-1 H1, IL-1rp2, IL1F9, IL36 gamma, IL36G
Gene Symbol
IL36G
UniProt
Additional IL-36 gamma/IL-1F9 Products
Product Documents for Human IL‑36 gamma /IL‑1F9 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL‑36 gamma /IL‑1F9 Antibody
For research use only
Related Research Areas
Citations for Human IL‑36 gamma /IL‑1F9 Antibody
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Customer Reviews for Human IL‑36 gamma /IL‑1F9 Antibody (2)
4 out of 5
2 Customer Ratings
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Application: Block/NeutralizeSample Tested: Cell SupernatantSpecies: HumanVerified Customer | Posted 05/01/2020
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
