Human IL-37/IL-1F7 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY1975
DY1975-05
Ancillary Products Available
Human IL-37 / IL-1F7 ELISA Standard Curve
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (2)

Human IL-37/IL-1F7 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-37 / IL-1F7. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Human IL-37 / IL-1F7 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-37/IL-1F7

Human interleukin 37, also named IL-1F7, FIL1 zeta, IL-1H4, and IL-1rp1, belongs to the IL-1 cytokine family. With the exception of IL-18 that maps to human chromosome 11, all IL-1 family members map to the same cluster on human chromosome 2. Mouse IL-37 has not been reported, but human IL-37 is active on mouse cells. Five isoforms (IL-37a through e) are generated through alternative exon usage and have distinct expression profiles. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-37 variants lack a typical signal peptide.

Long Name:
Interleukin 37
Entrez Gene IDs:
27178 (Human)
Alternate Names:
FIL1 zeta; FIL1; FIL1(ZETA); FIL1ZFIL1 zeta; IL-1F7; IL-1F7IL-1 zeta; IL1H4IL1F7 (canonical product IL-1F7b); IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1); IL-1RP1IL-1X protein; IL1RP1interleukin 1 family member 7; IL-1X; IL37; IL-37; interleukin 1 family, member 7 (zeta); interleukin 1, zeta; interleukin-1 family member 7; Interleukin-1 homolog 4; interleukin-1 superfamily z; Interleukin-1 zeta; Interleukin-1-related protein

Citations for Human IL-37/IL-1F7 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Interleukin-37 reduces inflammation and impairs phagocytosis of platelets in immune thrombocytopenia (ITP)
    Authors: Y Zhao, X Ni, P Xu, Q Liu, T Sun, X Liu, X Ji, J Qiu, J Li, S Wang, P Han, J Peng, M Hou, G Li
    Cytokine, 2019;125(0):154853.
    Species: Human
    Sample Types: Plasma
  2. Heightened systemic levels of anti-inflammatory cytokines in pulmonary tuberculosis and alterations following anti-tuberculosis treatment
    Authors: K Moideen, NP Kumar, R Bethunaick, VV Banurekha, D Nair, S Babu
    Cytokine, 2019;127(0):154929.
    Species: Human
    Sample Types: Plasma
  3. EASIX for prediction of survival in lower-risk myelodysplastic syndromes
    Authors: A Merz, U Germing, G Kobbe, J Kaivers, A Jauch, A Radujkovic, M Hummel, A Benner, M Merz, P Dreger, T Luft
    Blood Cancer J, 2019;9(11):85.
    Species: Human
    Sample Types: Plasma
  4. Interleukin-18 diagnostically distinguishes and pathogenically promotes human and murine macrophage activation syndrome
    Authors: ES Weiss, C Girard-Guy, D Holzinger, AA de Jesus, Z Tariq, J Picarsic, EJ Schiffrin, D Foell, AA Grom, S Ammann, S Ehl, T Hoshino, R Goldbach-M, C Gabay, SW Canna
    Blood, 2018;0(0):.
    Species: Human
    Sample Types: Serum
  5. Interleukin 37 promotes angiogenesis through TGF-? signaling
    Authors: M Zhao, Y Hu, J Jin, Y Yu, S Zhang, J Cao, Y Zhai, R Wei, J Shou, W Cai, S Liu, X Yang, GT Xu, J Yang, DB Corry, SB Su, X Liu, T Yang
    Sci Rep, 2017;7(1):6113.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

  1. Which isoforms of IL-37/IL-1F7 does this DuoSet ELISA detect?

    • This assay is calibrated against the IL-37a isoform. It is possible that the assay also detects additional isoforms, however, R&D Systems has not performed this characterization.

View all ELISA FAQs

Reviews for Human IL-37/IL-1F7 DuoSet ELISA

Average Rating: 4 (Based on 2 Reviews)

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Human IL-37/IL-1F7 DuoSet ELISA
By Anonymous on 08/11/2017
Application: Sample Tested: Serum and Plasma

Human IL-37/IL-1F7 DuoSet ELISA
By Anonymous on 02/16/2017
Application: Sample Tested: EDTA Plasma

After optimization we ran human EDTA-plasma samples at a 1:10. Due to huge variations in IL-37/IL-1F7 levels we added another standard at 4000pg/ml to the analysis. The high variations also makes it necessary to further dilute a number of patient samples in order to get values within the standard range.