Human IL-37/IL-1F7 DuoSet ELISA

R&D Systems | Catalog # DY1975

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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

31.2-2000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human IL-37/IL-1F7 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all IL-37/IL-1F7 ELISA Kits »

Product Summary for Human IL-37/IL-1F7 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-37 / IL-1F7. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human IL-37/IL-1F7 DuoSet ELISA

Human IL-37 / IL-1F7 ELISA Standard Curve

Human IL-37 / IL-1F7 ELISA Standard Curve

Kit Contents for Human IL-37/IL-1F7 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS:

(Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-37/IL-1F7

Human interleukin 37, also named IL-1F7, FIL1 zeta, IL-1H4, and IL-1rp1, belongs to the IL-1 cytokine family. With the exception of IL-18 that maps to human chromosome 11, all IL-1 family members map to the same cluster on human chromosome 2. Mouse IL-37 has not been reported, but human IL-37 is active on mouse cells. Five isoforms (IL-37a through e) are generated through alternative exon usage and have distinct expression profiles. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-37 variants lack a typical signal peptide.

Long Name

Interleukin 37

Alternate Names

FIL1 zeta, IL-1F7, IL37

Entrez Gene IDs

27178 (Human)

Gene Symbol

IL37

Additional IL-37/IL-1F7 Products

Product Documents for Human IL-37/IL-1F7 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IL-37/IL-1F7 DuoSet ELISA

For research use only

Related Research Areas

IL-1 Family

Citations for Human IL-37/IL-1F7 DuoSet ELISA

Customer Reviews for Human IL-37/IL-1F7 DuoSet ELISA (2)

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  • Human IL-37/IL-1F7 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 08/11/2017
    Human IL-37/IL-1F7 DuoSet ELISA DY1975
  • Human IL-37/IL-1F7 DuoSet ELISA
    Name: Jonatan Dereke
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 02/16/2017
    After optimization we ran human EDTA-plasma samples at a 1:10. Due to huge variations in IL-37/IL-1F7 levels we added another standard at 4000pg/ml to the analysis. The high variations also makes it necessary to further dilute a number of patient samples in order to get values within the standard range.
    Human IL-37/IL-1F7 DuoSet ELISA DY1975

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FAQs for Human IL-37/IL-1F7 DuoSet ELISA

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  • Q: Which isoforms of IL-37/IL-1F7 does this DuoSet ELISA detect?

    A: This assay is calibrated against the IL-37a isoform. It is possible that the assay also detects additional isoforms, however, R&D Systems has not performed this characterization.

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