Human IL-37/IL-1F7 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human IL-37 / IL-1F7 ELISA Standard Curve
Preparation and Storage
Human interleukin 37, also named IL-1F7, FIL1 zeta, IL-1H4, and IL-1rp1, belongs to the IL-1 cytokine family. With the exception of IL-18 that maps to human chromosome 11, all IL-1 family members map to the same cluster on human chromosome 2. Mouse IL-37 has not been reported, but human IL-37 is active on mouse cells. Five isoforms (IL-37a through e) are generated through alternative exon usage and have distinct expression profiles. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-37 variants lack a typical signal peptide.
Citations for Human IL-37/IL-1F7 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Interleukin-37 reduces inflammation and impairs phagocytosis of platelets in immune thrombocytopenia (ITP)
Authors: Y Zhao, X Ni, P Xu, Q Liu, T Sun, X Liu, X Ji, J Qiu, J Li, S Wang, P Han, J Peng, M Hou, G Li
Sample Types: Plasma
Heightened systemic levels of anti-inflammatory cytokines in pulmonary tuberculosis and alterations following anti-tuberculosis treatment
Authors: K Moideen, NP Kumar, R Bethunaick, VV Banurekha, D Nair, S Babu
Sample Types: Plasma
EASIX for prediction of survival in lower-risk myelodysplastic syndromes
Authors: A Merz, U Germing, G Kobbe, J Kaivers, A Jauch, A Radujkovic, M Hummel, A Benner, M Merz, P Dreger, T Luft
Blood Cancer J, 2019;9(11):85.
Sample Types: Plasma
Interleukin-18 diagnostically distinguishes and pathogenically promotes human and murine macrophage activation syndrome
Authors: ES Weiss, C Girard-Guy, D Holzinger, AA de Jesus, Z Tariq, J Picarsic, EJ Schiffrin, D Foell, AA Grom, S Ammann, S Ehl, T Hoshino, R Goldbach-M, C Gabay, SW Canna
Sample Types: Serum
Interleukin 37 promotes angiogenesis through TGF-? signaling
Authors: M Zhao, Y Hu, J Jin, Y Yu, S Zhang, J Cao, Y Zhai, R Wei, J Shou, W Cai, S Liu, X Yang, GT Xu, J Yang, DB Corry, SB Su, X Liu, T Yang
Sci Rep, 2017;7(1):6113.
Sample Types: Cell Culture Supernates
Which isoforms of IL-37/IL-1F7 does this DuoSet ELISA detect?
This assay is calibrated against the IL-37a isoform. It is possible that the assay also detects additional isoforms, however, R&D Systems has not performed this characterization.
DuoSet Ancillary Reagent Kits
Supplemental ELISA Products
Reviews for Human IL-37/IL-1F7 DuoSet ELISA
Average Rating: 4 (Based on 2 Reviews)
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After optimization we ran human EDTA-plasma samples at a 1:10. Due to huge variations in IL-37/IL-1F7 levels we added another standard at 4000pg/ml to the analysis. The high variations also makes it necessary to further dilute a number of patient samples in order to get values within the standard range.