Human IL-4R alpha APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB230A-100
FAB230A-025
Human IL-4R alpha  APC-conjugated Antibody in Data
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Product Details
Citations (5)
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Human IL-4R alpha APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑4 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-9 R, recombinant mouse IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-13 R alpha 1, or rhIL-13 R alpha 2 is observed.
Source
Monoclonal Mouse IgG2A Clone # 25463
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R
Gly24-His232
Accession # P24394
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IL‑4 R alpha  in Daudi Human Cell Line by Flow Cytometry. View Larger

Detection of IL‑4 R alpha in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-4 Ra APC-conjugated Monoclonal Antibody (Catalog # FAB230A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-4R alpha

Interleukin 4 receptor (IL-4 R) is the transmembrane ligand binding subunit of the heterodimeric IL-4 receptor complex. In the type I receptor complex, IL-4 R associates with the common gamma chain, whereas in the type II receptor complex, it associates with IL-13 R alpha 1. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.

Long Name
Interleukin 4 Receptor alpha
Entrez Gene IDs
3566 (Human); 16190 (Mouse)
Alternate Names
CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha

Product Datasheets

Citations for Human IL-4R alpha APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Enhancing the potency and specificity of engineered T cells for cancer treatment
    Authors: S Sukumaran, N Watanabe, P Bajgain, K Raja, S Mohammed, WE Fisher, MK Brenner, AM Leen, JF Vera
    Cancer Discov, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
  2. 4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells
    Authors: S Zilio, JL Vella, AC De la Fuen, PM Daftarian, DT Weed, A Kaifer, I Marigo, K Leone, V Bronte, P Serafini
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The TORC1-activated Proteins, p70S6K and GRB10, Regulate IL-4 Signaling and M2 Macrophage Polarization by Modulating Phosphorylation of Insulin Receptor Substrate-2
    Authors: Nicola M Heller
    J. Biol. Chem., 2016;291(48):24922-24930.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Absence of a role for interleukin-13 in inflammatory bowel disease.
    Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
    Eur J Immunol, 2014;44(2):370-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Presence of circulating Her2-reactive CD8 + T-cells is associated with lower frequencies of myeloid-derived suppressor cells and regulatory T cells, and better survival in older breast cancer patients.
    Authors: Bailur J, Gueckel B, Derhovanessian E, Pawelec G
    Breast Cancer Res, 0;17(0):34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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