Human IL-4R alpha Antibody

Name: Human IL-4R alpha Antibody
Brand: R&D Systems
SKU: MAB230-500
Rating: 4.5 (4 reviews)

Catalog #: MAB230
23 Citations 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB230-SP
MAB230-100
MAB230-500

Detection of IL‑4 R alpha in Daudi Human Cell Line by Flow Cytometry.

4 Images

View All Products for IL-4R alpha

Product Details

Citations (23)

FAQs

Supplemental Products

Reviews (4)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-4R alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑4 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-9 R, recombinant mouse IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-13 R alpha 1, or rhIL-13 R alpha 2 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 25463

Purification

Protein A or G purified from ascites

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R
Gly24-His232
Accession # P24394

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑4 R alpha (Catalog # 230-4R) under non-reducing conditions only

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 3-6 ng/mL in the presence of 0.2 ng/mL Recombinant Human IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of IL‑4 R alpha in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunohistochemistry

View Larger

IL‑4 R alpha in Human Lymph Node. IL-4 Ra was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry

View Larger

IL‑4 R alpha in HDLM‑2 Human Cell Line. IL-4 Ra was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization

View Larger

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha Antibody. Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑4 Ra Monoclonal Antibody (Catalog # MAB230). The ND₅₀ is typically 3-6 ng/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4R alpha

Interleukin 4 receptor (IL-4 R) is the transmembrane ligand binding subunit of the heterodimeric IL-4 receptor complex. In the type I receptor complex, IL-4 R associates with the common gamma chain, whereas in the type II receptor complex, it associates with IL-13 R alpha 1. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.

Long Name

Interleukin 4 Receptor alpha

Entrez Gene IDs

3566 (Human); 16190 (Mouse)

Alternate Names

CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-4R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
Filter your results:

Filter by:

The IL-4/IL-13 signaling axis promotes prostatic fibrosis
Authors: Q D'Arcy, M Gharaee-Ke, A Zhilin-Rot, JA Macoska
PLoS ONE, 2022-10-06;17(10):e0275064.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: IHC, Neutralization, Western Blot
IL-4 prevents adenosine-mediated immunoregulation by inhibiting CD39 expression
Authors: F Fang, W Cao, Y Mu, H Okuyama, L Li, J Qiu, CM Weyand, JJ Goronzy
JCI Insight, 2022-06-22;7(12):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Hyperinflammatory environment drives dysfunctional myeloid cell effector response to bacterial challenge in COVID-19
Authors: S Mairpady S, A Gómez-Meji, TA Schweizer, M Huemer, CC Chang, C Acevedo, J Bergada-Pi, C Vulin, DA Hofmaenner, TC Scheier, S Hertegonne, E Parietti, N Miroshniko, PD Wendel Gar, MP Hilty, PK Buehler, RA Schuepbach, SD Brugger, AS Zinkernage
PloS Pathogens, 2022-01-10;18(1):e1010176.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Platelets trigger perivascular mast cell degranulation to cause inflammatory responses and tissue injury
Authors: J Karhausen, HW Choi, KR Maddipati, JP Mathew, Q Ma, Y Boulaftali, RH Lee, W Bergmeier, SN Abraham
Sci Adv, 2020-03-18;6(12):eaay6314.
Species: Mouse
Sample Types:
Applications: In Vivo
Ex vivo physiological compression of human osteoarthritis cartilage modulates cellular and matrix components
Authors: P Dolzani, E Assirelli, L Pulsatelli, R Meliconi, E Mariani, S Neri
PLoS ONE, 2019-09-24;14(9):e0222947.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Allergen-specific IgG antibodies signaling via Fc?RIIb promote food tolerance
Authors: OT Burton, JM Tamayo, AJ Stranks, KJ Koleoglou, HC Oettgen
J. Allergy Clin. Immunol., 2017-05-04;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
IL-4 primes the dynamics of breast cancer progression via DUSP4 inhibition
Authors: M Gaggianesi, A Turdo, A Chinnici, E Lipari, T Apuzzo, A Benfante, I Sperduti, S Di Franco, S Meraviglia, E Lo Presti, F Dieli, V Caputo, G Militello, S Vieni, G Stassi, M Todaro
Cancer Res., 2017-04-11;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells.
Authors: Reece P, Gauvreau G, Sehmi R, Denburg J
PLoS ONE, 2014-06-27;9(6):e100734.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
PLoS ONE, 2014-05-12;9(5):e96925.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, ICC, IHC-Fr
Absence of a role for interleukin-13 in inflammatory bowel disease.
Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
Eur J Immunol, 2014-02-01;44(2):370-85.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Construction and application of elastin like polypeptide containing IL-4 receptor targeting peptide.
Authors: Sarangthem, Vijaya, Cho, Eun A, Bae, Sang Mun, Singh, Thoudam, Kim, Sun-Ji, Kim, Soyoun, Jeon, Won Bae, Lee, Byung-He, Park, Rang-Woo
PLoS ONE, 2013-12-10;8(12):e81891.
Species: Human
Sample Types: Whole Cells
Applications: Blocking

Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4.
Authors: Volonte A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C
J Immunol, 2013-11-25;192(1):523-32.
Species: Human
Sample Types: Whole Cells
Applications: IHC, Neutralization

Platelet ITAM signaling is critical for vascular integrity in inflammation.
Authors: Boulaftali, Yacine, Hess, Paul R, Getz, Todd M, Cholka, Agnieszk, Stolla, Moritz, Mackman, Nigel, Owens, A Philli, Ware, Jerry, Kahn, Mark L, Bergmeier, Wolfgang
J Clin Invest, 2013-01-25;123(2):908-16.
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay

Hrs Recognizes a Hydrophobic Amino Acid Cluster in Cytokine Receptors during Ubiquitin-independent Endosomal Sorting.
Authors: Amano Y, Yamashita Y, Kojima K, Yoshino K, Tanaka N, Sugamura K, Takeshita T
J. Biol. Chem., 2011-03-01;286(17):15458-72.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC, Neutralization

Fatty acid amide hydrolase in prostate cancer: association with disease severity and outcome, CB1 receptor expression and regulation by IL-4.
Authors: Thors L, Bergh A, Persson E, Hammarsten P, Stattin P, Egevad L, Granfors T, Fowler CJ
PLoS ONE, 2010-08-19;5(8):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function.
Authors: Hu A, Fatma S, Cao J, Grunstein JS, Nino G, Grumbach Y, Grunstein MM
Am. J. Physiol. Lung Cell Mol. Physiol., 2009-02-27;296(5):L790-803.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
J. Allergy Clin. Immunol., 2008-12-03;123(1):59-66.e4.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Interleukin-4 induces the activation and collagen production of cultured human intrahepatic fibroblasts via the STAT-6 pathway.
Authors: Aoudjehane L, Pissaia A, Scatton O, Podevin P, Massault PP, Chouzenoux S, Soubrane O, Calmus Y, Conti F
Lab. Invest., 2008-07-14;88(9):973-85.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway.
Authors: Aoudjehane L, Podevin P, Scatton O, Jaffray P, Dusanter-Fourt I, Feldmann G, Massault PP, Grira L, Bringuier A, Dousset B, Chouzenoux S, Soubrane O, Calmus Y, Conti F
FASEB J., 2007-02-05;21(7):1433-44.
Species: Human
Sample Types: Whole Cells
Applications: ICC

Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
J. Immunol., 2002-11-01;169(9):5244-51.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
Authors: Hirst SJ, Hallsworth MP, Peng Q
Am. J. Respir. Crit. Care Med., 2002-04-15;165(8):1161-71.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
J. Leukoc. Biol., 2001-10-01;70(4):617-23.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
Eur. J. Immunol., 2001-04-01;31(4):1037-46.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization