Human IL-4R alpha Antibody

Catalog # Availability Size / Price Qty
MAB230-SP
MAB230-100
MAB230-500
Human IL-4R alpha  Antibody in Data
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Product Details
Citations (19)
FAQs
Supplemental Products
Reviews (1)

Human IL-4R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑4 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-9 R, recombinant mouse IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-13 R alpha 1, or rhIL-13 R alpha 2 is observed.
Source
Monoclonal Mouse IgG2A Clone # 25463
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R
Gly24-His232
Accession # P24394
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑4 R alpha (Catalog # 230-4R) under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 3-6 ng/mL in the presence of 0.2 ng/mL Recombinant Human IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of IL‑4 R alpha  in Daudi Human Cell Line by Flow Cytometry. View Larger

Detection of IL‑4 R alpha in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry IL‑4 R alpha  in HDLM‑2 Human Cell Line. View Larger

IL‑4 R alpha in HDLM‑2 Human Cell Line. IL-4 Ra was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry IL‑4 R alpha  in Human Lymph Node. View Larger

IL‑4 R alpha in Human Lymph Node. IL-4 Ra was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha  Antibody. View Larger

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha Antibody. Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑4 Ra Monoclonal Antibody (Catalog # MAB230). The ND50is typically 3-6 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4R alpha

Interleukin 4 receptor (IL-4 R) is the transmembrane ligand binding subunit of the heterodimeric IL-4 receptor complex. In the type I receptor complex, IL-4 R associates with the common gamma chain, whereas in the type II receptor complex, it associates with IL-13 R alpha 1. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.

Long Name
Interleukin 4 Receptor alpha
Entrez Gene IDs
3566 (Human); 16190 (Mouse)
Alternate Names
CD124 antigen; CD124; IL-4 R alpha; IL-4 receptor subunit alpha; IL4R alpha; IL-4R alpha; IL-4R subunit alpha; IL4R; IL-4Ra; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha

Product Datasheets

Citations for Human IL-4R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Ex vivo physiological compression of human osteoarthritis cartilage modulates cellular and matrix components
    Authors: P Dolzani, E Assirelli, L Pulsatelli, R Meliconi, E Mariani, S Neri
    PLoS ONE, 2019;14(9):e0222947.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. IL-4 primes the dynamics of breast cancer progression via DUSP4 inhibition
    Authors: M Gaggianesi, A Turdo, A Chinnici, E Lipari, T Apuzzo, A Benfante, I Sperduti, S Di Franco, S Meraviglia, E Lo Presti, F Dieli, V Caputo, G Militello, S Vieni, G Stassi, M Todaro
    Cancer Res., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Allergen-specific IgG antibodies signaling via Fc?RIIb promote food tolerance
    Authors: OT Burton, JM Tamayo, AJ Stranks, KJ Koleoglou, HC Oettgen
    J. Allergy Clin. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells.
    Authors: Reece P, Gauvreau G, Sehmi R, Denburg J
    PLoS ONE, 2014;9(6):e100734.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Absence of a role for interleukin-13 in inflammatory bowel disease.
    Authors: Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T
    Eur J Immunol, 2014;44(2):370-85.
  6. Cancer-initiating cells from colorectal cancer patients escape from T cell-mediated immunosurveillance in vitro through membrane-bound IL-4.
    Authors: Volonte A, Di Tomaso T, Spinelli M, Todaro M, Sanvito F, Albarello L, Bissolati M, Ghirardelli L, Orsenigo E, Ferrone S, Doglioni C, Stassi G, Dellabona P, Staudacher C, Parmiani G, Maccalli C
    J Immunol, 2014;192(1):523-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  7. Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
    Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
    PLoS ONE, 2014;9(5):e96925.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Construction and application of elastin like polypeptide containing IL-4 receptor targeting peptide.
    Authors: Sarangthem, Vijaya, Cho, Eun A, Bae, Sang Mun, Singh, Thoudam, Kim, Sun-Ji, Kim, Soyoun, Jeon, Won Bae, Lee, Byung-He, Park, Rang-Woo
    PLoS ONE, 2013;8(12):e81891.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  9. Platelet ITAM signaling is critical for vascular integrity in inflammation.
    Authors: Boulaftali, Yacine, Hess, Paul R, Getz, Todd M, Cholka, Agnieszk, Stolla, Moritz, Mackman, Nigel, Owens, A Philli, Ware, Jerry, Kahn, Mark L, Bergmeier, Wolfgang
    J Clin Invest, 2013;123(2):908-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  10. Hrs Recognizes a Hydrophobic Amino Acid Cluster in Cytokine Receptors during Ubiquitin-independent Endosomal Sorting.
    Authors: Amano Y, Yamashita Y, Kojima K, Yoshino K, Tanaka N, Sugamura K, Takeshita T
    J. Biol. Chem., 2011;286(17):15458-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Fatty acid amide hydrolase in prostate cancer: association with disease severity and outcome, CB1 receptor expression and regulation by IL-4.
    Authors: Thors L, Bergh A, Persson E, Hammarsten P, Stattin P, Egevad L, Granfors T, Fowler CJ
    PLoS ONE, 2010;5(8):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Th2 cytokine-induced upregulation of 11beta-hydroxysteroid dehydrogenase-1 facilitates glucocorticoid suppression of proasthmatic airway smooth muscle function.
    Authors: Hu A, Fatma S, Cao J, Grunstein JS, Nino G, Grumbach Y, Grunstein MM
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(5):L790-803.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response.
    Authors: Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Allergy Clin. Immunol., 2009;123(1):59-66.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Interleukin-4 induces the activation and collagen production of cultured human intrahepatic fibroblasts via the STAT-6 pathway.
    Authors: Aoudjehane L, Pissaia A, Scatton O, Podevin P, Massault PP, Chouzenoux S, Soubrane O, Calmus Y, Conti F
    Lab. Invest., 2008;88(9):973-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway.
    Authors: Aoudjehane L, Podevin P, Scatton O, Jaffray P, Dusanter-Fourt I, Feldmann G, Massault PP, Grira L, Bringuier A, Dousset B, Chouzenoux S, Soubrane O, Calmus Y, Conti F
    FASEB J., 2007;21(7):1433-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
    Authors: Hirst SJ, Hallsworth MP, Peng Q
    Am. J. Respir. Crit. Care Med., 2002;165(8):1161-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001;70(4):617-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
    Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
    Eur. J. Immunol., 2001;31(4):1037-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Human IL-4R alpha Antibody

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Human IL-4 R alpha Antibody
By Anonymous on 12/06/2017
Application: B/N Sample Tested: Recombinant protein Species: Human