Human IL‑5 PE‑conjugated Antibody

R&D Systems | Catalog # IC605P

R&D Systems
Discontinued Product
IC605P has been discontinued. An alternative/replacement product is available: IC605G, IC605N, IC605S, IC605T, IC605U. View all IL-5 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 9906
View all IL-5 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑5
Ile20-Ser134
Accession # P05113

Specificity

Detects human IL-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL‑5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL‑5 PE‑conjugated Antibody

Detection of IL-5 antibody in Human Peripheral Blood Mononuclear Cells antibody by Flow Cytometry.

Detection of IL-5 in Human Peripheral Blood Mononuclear Cells by Flow Cytometry.

Resting (panel A) and PMA/ionomycin-activated (panel B) human peripheral blood mononuclear cells were stained with Mouse Anti-Human IL-5 PE-conjugated Monoclonal Antibody (Catalog # IC605P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human IL‑5 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.5 µL/106 cells
Sample: Resting and PMA/ionomycin-activated human peripheral blood mononuclear cells

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-5

Interleukin-5 (IL-5) is a 20-21 kDa secreted glycoprotein that belongs to the alpha -helical group of cytokines (1-3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein that contains both N- and O-linked carbohydrate. Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, group 2 ILCs, macrophages, and IL‑2‑stimulated invariant natural killer T cells (iNKT) (1, 3, 6-9). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha   antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).

References

  1. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
  2. Takatsu, K. (2014) Front. Immunol. 5:369.
  3. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  4. Minamitake, Y. et al. (1990) J. Biochem. 107:292.
  5. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
  6. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
  7. Hazenberg, M.D.  and H. Spits (2014) Blood 124:700.
  8. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
  9. Li, X. et al. (2016) Biochem. Biophys. Res. Commun. 473:939.
  10. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  11. Broughton, S.E. et al. (2015) Cytokine 74:247.
  12. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  13. Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
  14. Tavernier, J. et al. (2000) Blood 95:1600.
  15. Kopf, M. et al. (1996) Immunity 4:15.
  16. Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
  17. Denburg, J. A. et al. (1991) Blood 77:1462.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 100034199 (Equine); 493803 (Feline)

Gene Symbol

IL5

UniProt

P05113

Additional IL-5 Products

Product Documents for Human IL‑5 PE‑conjugated Antibody

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Product Specific Notices for Human IL‑5 PE‑conjugated Antibody

For research use only

Citations for Human IL‑5 PE‑conjugated Antibody

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