Human IL-6R alpha Biotinylated Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Product Details
Citations (8)
Supplemental Products
Reviews (1)

Human IL-6R alpha Biotinylated Antibody Summary

Species Reactivity
Detects human IL-6R alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) IL‑6, recombinant mouse IL-6, recombinant porcine IL-6, and rhgp130 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
Accession # P08887
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human IL-6R alpha (Catalog # 227-SR)

Human IL-6R alpha Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human IL-6R alpha Antibody (Catalog # MAB227R)

Capture Reagent: Human IL-6R alpha Antibody (Catalog # MAB227)

Standard: Recombinant Human IL-6R alpha Protein (Catalog # 227-SR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6R alpha

The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

  1. Yamasaki et al. (1988) Science 241:825.
  2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
  3. Hibi et al. (1990) Cell 63:1149.
  4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
  5. Novick et al. (1989) J. Exp. Med. 170:1409.
  6. Yasukawa et al. (1990) J. Biochem. 108:673.
  7. Saito et al. (1991) J. Immunology 147:168.
Long Name
Interleukin 6 Receptor alpha
Entrez Gene IDs
3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names
CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

Product Datasheets

You must select a language.


Citations for Human IL-6R alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

  1. Interleukin-6 controls recycling and degradation, but not internalization of its receptors
    Authors: CM Flynn, B Kespohl, T Daunke, Y Garbers, S Düsterhöft, S Rose-John, J Haybaeck, J Lokau, S Aparicio-S, C Garbers
    The Journal of Biological Chemistry, 2021-02-19;0(0):100434.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  2. Exploitation of Elevated Extracellular ATP to Specifically Direct Antibody to Tumor Microenvironment
    Authors: F Mimoto, K Tatsumi, S Shimizu, S Kadono, K Haraya, M Nagayasu, Y Suzuki, E Fujii, M Kamimura, A Hayasaka, H Kawauchi, K Ohara, M Matsushita, T Baba, H Susumu, T Sakashita, T Muraoka, K Aso, H Katada, E Tanaka, K Nakagawa, M Hasegawa, M Ayabe, T Yamamoto, S Tanba, T Ishiguro, T Kamikawa, T Nambu, T Kibayashi, Y Azuma, Y Tomii, A Kato, K Ozeki, N Murao, M Endo, J Kikuta, M Kamata-Sak, M Ishii, K Hattori, T Igawa
    Cell Reports, 2020-12-22;33(12):108542.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Capture
  3. Inhibitory FcgammaRIIb-Mediated Soluble Antigen Clearance from Plasma by a pH-Dependent Antigen-Binding Antibody and Its Enhancement by Fc Engineering.
    Authors: Iwayanagi Y, Igawa T, Maeda A, Haraya K, Wada N, Shibahara N, Ohmine K, Nambu T, Nakamura G, Mimoto F, Katada H, Ito S, Tachibana T, Jishage K, Hattori K
    J Immunol, 2015-08-28;195(7):3198-205.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Development
  4. A disintegrin and metalloprotease 17 dynamic interaction sequence, the sweet tooth for the human interleukin 6 receptor.
    Authors: Dusterhoft S, Hobel K, Oldefest M, Lokau J, Waetzig G, Chalaris A, Garbers C, Scheller J, Rose-John S, Lorenzen I, Grotzinger J
    J Biol Chem, 2014-04-30;289(23):16336-48.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  5. Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa.
    Authors: Wood MW, Breitschwerdt EB, Gookin JL
    Infect. Immun., 2010-11-29;79(2):708-15.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  7. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002-11-01;169(9):5244-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  8. Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
    Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
    Immunity, 2001-06-01;14(6):705-14.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human IL-6R alpha Biotinylated Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human IL-6R alpha Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human IL-6 R alpha Biotinylated Antibody
By Anonymous on 06/01/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human IL-6 receptor alpha was measured in human plasma samples using an immunoassay comprising of this antibody and MAB227. This antibody was used as the detection and the MAB227 was used as the solid phase capture molecule.