Human IL-6R alpha Antibody

AF-227-NA 5 Citations Datasheet / COA / SDS

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AF-227-NA
AF-227-SP

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Human IL-6R alpha Antibody in Western Blot

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-6R alpha Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-6R alpha in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
Leu20-Asp358
Accession # P08887

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Neutralization

Measured by its ability to neutralize IL-6R alpha-mediated inhibition of proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND₅₀) is typically 0.2-1.6 µg/mL in the presence of 30 ng/mL Recombinant Human IL-6R alpha and 30 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Recombinant Human IL-6R alpha by Western Blot. Western blot shows 5 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR), Recombinant Mouse IL-6R alpha (Catalog # 1830-SR) and Recombinant Rat IL-6 R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Neutralization

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IL-6R alpha Enhancement of IL‑6‑dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6R alpha Antibody. Recombinant Human IL-6R alpha (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA). The ND₅₀is typically 0.2-1.6 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

References

Yamasaki et al. (1988) Science 241:825.
Baumann et al. (1990) J. Biol. Chem. 265:19853.
Hibi et al. (1990) Cell 63:1149.
Schooltink et al. (1991) Eur. J. Biochem. 277:659.
Novick et al. (1989) J. Exp. Med. 170:1409.
Yasukawa et al. (1990) J. Biochem. 108:673.
Saito et al. (1991) J. Immunology 147:168.

Long Name

Interleukin 6 Receptor alpha

Entrez Gene IDs

3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)

Alternate Names

CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

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Citations for Human IL-6R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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An autoinflammatory neurological disease due to interleukin 6 hypersecretion.
Authors: Salsano, Ettore, Rizzo, Ambra, Bedini, Gloria, Bernard, Loris, Dall'olio, Valentin, Volorio, Sara, Lazzaroni, Monica, Ceccherini, Isabella, Lazarevic, Dejan, Cittaro, Davide, Stupka, Elia, Paterra, Rosina, Farina, Laura, Savoiardo, Mario, Pareyson, Davide, Sciacca, Francesc
J Neuroinflammation, 2013;10(0):29.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
J. Immunol., 2011;186(7):4175-82.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Dig. Dis. Sci., 2007;52(10):2775-83.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy.
Authors: Podewski EK, Hilfiker-Kleiner D, Hilfiker A, Morawietz H, Lichtenberg A, Wollert KC, Drexler H
Circulation, 2003;107(6):798-802.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
Protection of CLL B cells by a follicular dendritic cell line is dependent on induction of Mcl-1.
Authors: Pedersen IM, Kitada S, Leoni LM, Zapata JM, Karras JG, Tsukada N, Kipps TJ, Choi YS, Bennett F, Reed JC
Blood, 2002;100(5):1795-801.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization