Human Integrin  alpha 2/CD49b Biotinylated Antibody

R&D Systems | Catalog # BAM1233

Clone HAS3 was used by HLDA to establish CD designation
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Immunoprecipitation

Cited:

Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2A Clone # HAS3
View all Integrin alpha 2/CD49b Primary Antibodies »

Product Specifications

Immunogen

Human keratinocytes

Specificity

Detects human Integrin alpha 2/CD49b.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Integrin  alpha 2/CD49b Biotinylated Antibody

Integrin  alpha 2/CD49b in HT1080 Human Cell Line.

Integrin  alpha 2/CD49b was detected in immersion fixed HT1080 human fibrosarcoma cell line (positive staining) and Daudi human Burkitt's lymphoma cell line (negative staining) using Mouse Anti-Human Integrin  alpha 2/CD49b Biotinylated Monoclonal Antibody (Catalog # BAM1233) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human Integrin  alpha 2/CD49b Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: HT1080 human fibrosarcoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT1080 human fibrosarcoma cell line

Immunoprecipitation

Tenchini, M.L. et al. (1993) Cell Adhesion Communication 1:55.

Reviewed Applications

Read 1 review rated 5 using BAM1233 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 2/CD49b

Integrin alpha 2 is one of twelve integrin family alpha subunits that share the beta 1 subunit (1‑3). Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E-cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8‑10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non-hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
  4. Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
  5. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
  6. Sarratt, K.L. et al. (2005) Blood 106:1268.
  7. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
  8. Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
  9. McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
  10. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
  11. Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
  12. Edelson, B.T. et al. 2006) Blood 107:143.

Alternate Names

CD49b, ITGA2, VLA-2 alpha

Entrez Gene IDs

3673 (Human); 16398 (Mouse)

Gene Symbol

ITGA2

Additional Integrin alpha 2/CD49b Products

Product Documents for Human Integrin  alpha 2/CD49b Biotinylated Antibody

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Product Specific Notices for Human Integrin  alpha 2/CD49b Biotinylated Antibody

For research use only

Citations for Human Integrin  alpha 2/CD49b Biotinylated Antibody

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  • Human Integrin  alpha 2/CD49b Biotinylated Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 02/22/2022
    Human Integrin  alpha 2/CD49b Biotinylated Antibody BAM1233

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