Human Integrin  alpha 2/CD49b Antibody

Clone HAS3 was used by HLDA to establish CD designation.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin alpha 2/CD49b.
  • Source
    Monoclonal Mouse IgG2A Clone # HAS3
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human keratinocytes
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunoprecipitation
    Tenchini, M.L. et al. (1993) Cell Adhesion Communication 1:55.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    15-30 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Integrin  alpha 2/CD49b in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin  alpha 2/CD49b Monoclonal Antibody (Catalog # MAB1233, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
Integrin  alpha 2/CD49b in HT1080 Human Cell Line. Integrin  alpha 2/CD49b was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin  alpha 2/CD49b Monoclonal Antibody (Catalog # MAB1233) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 2/CD49b
Integrin alpha 2 is one of twelve integrin family alpha subunits that share the beta 1 subunit (1‑3). Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E-cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8‑10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non-hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).
  • References:
    1. Takada, Y. et al. (2007) Genome Biol. 8:215.
    2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
    3. Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
    4. Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
    5. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
    6. Sarratt, K.L. et al. (2005) Blood 106:1268.
    7. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
    8. Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
    9. McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
    10. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
    11. Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
    12. Edelson, B.T. et al. 2006) Blood 107:143.
  • Entrez Gene IDs:
    3673 (Human); 16398 (Mouse)
  • Alternate Names:
    alpha-2 subunit; CD49b antigen; CD49b; Integrin alpha 2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); ITGA2; VLA 2; VLA-2 alpha
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