Human Integrin  alpha 2/CD49b Antibody

Clone HAS3 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB1233
MAB1233-SP
Detection of Integrin  alpha 2/CD49b in HT1080 Human Cell Line by Flow Cytometry.
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Product Details
Citations (6)
FAQs
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Human Integrin  alpha 2/CD49b Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 2/CD49b.
Source
Monoclonal Mouse IgG2A Clone # HAS3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human keratinocytes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunoprecipitation
Tenchini, M.L. et al. (1993) Cell Adhesion Communication 1:55.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
15-30 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Integrin a2/CD49b antibody in HT1080 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 2/CD49b in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin a2/CD49b Monoclonal Antibody (Catalog # MAB1233, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry Integrin a2/CD49b antibody in HT1080 Human Cell Line by Immunocytochemistry (ICC). View Larger

Integrin alpha 2/CD49b in HT1080 Human Cell Line. Integrin a2/CD49b was detected in immersion fixed HT1080 human fibrosarcoma cell line using Mouse Anti-Human Integrin a2/CD49b Monoclonal Antibody (Catalog # MAB1233) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2/CD49b

Integrin alpha 2 is one of twelve integrin family alpha subunits that share the beta 1 subunit (1‑3). Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside-out) signaling convert it to its most active, extended and open conformation (1, 2). The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2. The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E-cadherin, and collagen-like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan-1 or HGF R/c-Met, and antagonized by crosstalk with Integrin alpha 1 beta 1 (8‑10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non-hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and costimulates mast cell activation, triggering neutrophil influx (4, 12).

References
  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
  4. Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
  5. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
  6. Sarratt, K.L. et al. (2005) Blood 106:1268.
  7. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
  8. Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
  9. McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
  10. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
  11. Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
  12. Edelson, B.T. et al. 2006) Blood 107:143.
Entrez Gene IDs
3673 (Human); 16398 (Mouse)
Alternate Names
alpha-2 subunit; CD49b antigen; CD49b; Integrin alpha 2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); ITGA2; VLA 2; VLA-2 alpha

Product Datasheets

Citations for Human Integrin  alpha 2/CD49b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Glycosylation modulates melanoma cell alpha2beta1 and alpha3beta1 integrin interactions with type IV collagen.
    Authors: Stawikowski M, Aukszi B, Stawikowska R, Cudic M, Fields G
    J Biol Chem, 2014;289(31):21591-604.
    Species: Human
    Sample Types: Whole Cells
  2. In vitro cultured stromal cells from human tonsils display a distinct phenotype and induce B cell adhesion and proliferation.
    Authors: Lisignoli G, Monaco M, Facchini A, Toneguzzi S, Cattini L, Hilbert D, Lavaroni S, Belvedere O, Degrassi A
    Eur J Immunol, 0;26(1):17-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Cell surface expression and functional significance of adhesion molecules on human myeloma-derived cell lines.
    Authors: Kim I, Uchiyama H, Chauhan D, Anderson K
    Br J Haematol, 0;87(3):483-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Direct binding of collagen to the I domain of integrin alpha 2 beta 1 (VLA-2, CD49b/CD29) in a divalent cation-independent manner.
    Authors: Kamata T, Takada Y
    J Biol Chem, 0;269(42):26006-10.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization
  5. Immunohistochemical demonstration of cellular antigens of the cat defined by anti-human antibodies.
    Authors: Joling P, Broekhuizen R, de Weger R, Rottier P, Egberink H
    Vet Immunol Immunopathol, 0;53(1):115-27.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Functional Assay
  6. Monkey rotavirus binding to alpha2beta1 integrin requires the alpha2 I domain and is facilitated by the homologous beta1 subunit.
    Authors: Londrigan S, Graham K, Takada Y, Halasz P, Coulson B
    J Virol, 0;77(17):9486-501.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Isotype Controls

Mouse IgG2A Isotype Control

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Secondary Antibodies

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