Human Integrin alpha V beta 3 PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Integrin alpha V beta 3 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Integrin aV beta 3 PE-conjugated Monoclonal Antibody (Catalog # FAB3050P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha V beta 3
Integrin alpha V beta 3, together with Integrin alpha IIb beta 3, constitute the only known beta 3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N-terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alpha V subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alpha V beta 3 (4, 10). The 962 aa human alpha V ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow alpha V while the 685 aa human beta 3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta 3.
- Hynes, R. O. (2002) Cell 110:673.
- Serini, G. et al. (2006) Exp. Cell Res. 312:651.
- Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
- Xiong, J. et al. (2001) Science 294:339.
- Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
- vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
- Djaffar, I. et al. (1994) Biochem. J. 300:67.
- McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
- Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
- Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
- Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
- Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
Product Datasheets
Citations for Human Integrin alpha V beta 3 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Genome-scale analysis identifies paralog lethality as a vulnerability of chromosome 1p loss in cancer
Authors: Srinivas R. Viswanathan, Marina F. Nogueira, Colin G. Buss, John M. Krill-Burger, Mathias J. Wawer, Edyta Malolepsza et al.
Nature Genetics
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A microglia clonal inflammatory disorder in Alzheimer's Disease
Authors: Vicario, R;Fragkogianni, S;Weber, L;Lazarov, T;Hu, Y;Hayashi, SY;Craddock, BP;Socci, ND;Alberdi, A;Baako, A;Ay, O;Ogishi, M;Lopez-Rodrigo, E;Kappagantula, R;Viale, A;Iacobuzio-Donahue, CA;Zhou, T;Ransohoff, RM;Chesworth, R;Bank, NB;Abdel-Wahab, O;Boisson, B;Elemento, O;Casanova, JL;Miller, WT;Geissmann, F;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Peptidoglycan from Bacillus anthracis Inhibits Human Macrophage Efferocytosis in Part by Reducing Cell Surface Expression of MERTK and TIM-3
Authors: JS Mytych, Z Pan, C Lopez-Davi, N Redinger, C Lawrence, J Ziegler, N Popescu, JA James, AD Farris
bioRxiv : the preprint server for biology, 2023-05-10;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Evaluation of a dimeric-cRGD peptide for targeted PET-CT imaging of peripheral angiogenesis in diabetic mice
Authors: J Hedhli, SLL Slania, A P?oska, A Czerwinski, CJ Konopka, M Wozniak, M Banach, IT Dobrucki, L Kalinowski, LW Dobrucki
Sci Rep, 2018-03-29;8(1):5401.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, ICC -
Synthesis, Chemical Characterization and Multiscale Biological Evaluation of a Dimeric-cRGD Peptide for Targeted Imaging of ? V ? 3 Integrin Activity
Authors: J Hedhli, A Czerwinski, M Schuelke, A P?oska, P Sowinski, L Hood, SB Mamer, JA Cole, P Czaplewska, M Banach, IT Dobrucki, L Kalinowski, P Imoukhuede, LW Dobrucki
Sci Rep, 2017-06-09;7(1):3185.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.
Authors: Chang MW, Lo JM, Juan HF, Chang HY, Chuang CY
PLoS ONE, 2012-05-24;7(5):e37935.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
J. Exp. Clin. Cancer Res., 2012-03-23;31(0):26.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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