Human Integrin  alpha V beta 3 PE-conjugated Antibody FAB3050P: R&D Systems

Human Integrin  alpha V beta 3 PE-conjugated Antibody

(3 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin alpha V beta 3.
  • Source
    Monoclonal Mouse IgG1 Clone # 23C6
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human osteoclasts
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Integrin  alpha V beta 3 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Integrin  alpha V beta 3 PE‑conjugated Monoclonal Antibody (Catalog # FAB3050P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha V beta 3
Integrin alpha V beta 3, together with Integrin  alpha IIb beta 3, constitute the only known beta 3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N-terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alpha V subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alpha V beta 3 (4, 10). The 962 aa human alpha V ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow  alpha V while the 685 aa human beta 3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta 3.
  • References:
    1. Hynes, R. O. (2002) Cell 110:673.
    2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
    3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
    4. Xiong, J. et al. (2001) Science 294:339.
    5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
    6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
    7. Djaffar, I. et al. (1994) Biochem. J. 300:67.
    8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
    9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
    10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
    11. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
    12. Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
  • Entrez Gene IDs:
    3685 (Human)
  • Alternate Names:
    antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V beta 3; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; Vitronectin receptor subunit alpha; VNRADKFZp686A08142
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Synthesis, Chemical Characterization and Multiscale Biological Evaluation of a Dimeric-cRGD Peptide for Targeted Imaging of ? V ? 3 Integrin Activity
    Authors: J Hedhli, A Czerwinski, M Schuelke, A P?oska, P Sowinski, L Hood, SB Mamer, JA Cole, P Czaplewska, M Banach, IT Dobrucki, L Kalinowski, P Imoukhuede, LW Dobrucki
    Sci Rep, 2017;7(1):3185.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.
    Authors: Chang MW, Lo JM, Juan HF, Chang HY, Chuang CY
    PLoS ONE, 2012;7(5):e37935.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
    Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
    J. Exp. Clin. Cancer Res., 2012;31(0):26.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human Integrin  alpha V beta 3 PE-conjugated Antibody FAB3050P
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Flow Cytometry Human Integrin  alpha V beta 3 PE-conjugated Antibody FAB3050P
Flow Cytometry: Human Integrin  alpha V beta 3 PE-conjugated Antibody [FAB3050P]

Summary

ApplicationFlow Cytometry
Sample TestedBone marrow cells
SpeciesHuman

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