Human Integrin  alpha V beta 3 PE-conjugated Antibody

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Detection of Integrin  alpha V beta 3 in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (5)
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Human Integrin  alpha V beta 3 PE-conjugated Antibody Summary

Species Reactivity
Detects human Integrin alpha V beta 3.
Monoclonal Mouse IgG1 Clone # 23C6
Protein A or G purified from hybridoma culture supernatant
Human osteoclasts
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin aV beta 3 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of Integrin alpha V beta 3 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Integrin aV beta 3 PE-conjugated Monoclonal Antibody (Catalog # FAB3050P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha V beta 3

Integrin alpha V beta 3, together with Integrin  alpha IIb beta 3, constitute the only known beta 3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N-terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alpha V subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alpha V beta 3 (4, 10). The 962 aa human alpha V ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow  alpha V while the 685 aa human beta 3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta 3.

  1. Hynes, R. O. (2002) Cell 110:673.
  2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
  3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
  4. Xiong, J. et al. (2001) Science 294:339.
  5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
  6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
  7. Djaffar, I. et al. (1994) Biochem. J. 300:67.
  8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
  9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
  11. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
  12. Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
Entrez Gene IDs
3685 (Human)
Alternate Names
antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V beta 3; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; Vitronectin receptor subunit alpha; VNRADKFZp686A08142

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Citations for Human Integrin  alpha V beta 3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Peptidoglycan from Bacillus anthracis Inhibits Human Macrophage Efferocytosis in Part by Reducing Cell Surface Expression of MERTK and TIM-3
    Authors: JS Mytych, Z Pan, C Lopez-Davi, N Redinger, C Lawrence, J Ziegler, N Popescu, JA James, AD Farris
    bioRxiv : the preprint server for biology, 2023-05-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Evaluation of a dimeric-cRGD peptide for targeted PET-CT imaging of peripheral angiogenesis in diabetic mice
    Authors: J Hedhli, SLL Slania, A P?oska, A Czerwinski, CJ Konopka, M Wozniak, M Banach, IT Dobrucki, L Kalinowski, LW Dobrucki
    Sci Rep, 2018-03-29;8(1):5401.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  3. Synthesis, Chemical Characterization and Multiscale Biological Evaluation of a Dimeric-cRGD Peptide for Targeted Imaging of ? V ? 3 Integrin Activity
    Authors: J Hedhli, A Czerwinski, M Schuelke, A P?oska, P Sowinski, L Hood, SB Mamer, JA Cole, P Czaplewska, M Banach, IT Dobrucki, L Kalinowski, P Imoukhuede, LW Dobrucki
    Sci Rep, 2017-06-09;7(1):3185.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Combination of RGD compound and low-dose paclitaxel induces apoptosis in human glioblastoma cells.
    Authors: Chang MW, Lo JM, Juan HF, Chang HY, Chuang CY
    PLoS ONE, 2012-05-24;7(5):e37935.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer.
    Authors: Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E
    J. Exp. Clin. Cancer Res., 2012-03-23;31(0):26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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Human Integrin  alpha V beta 3 PE-conjugated Antibody
By Adam Guess on 08/01/2016
Application: Flow Sample Tested: Bone marrow cells Species: Human