Human Integrin beta 1/CD29 PE‑conjugated Antibody

Name: Human Integrin beta 1/CD29 PE‑conjugated Antibody
Brand: R&D Systems
SKU: FAB17781P
Price: 349 USD
Availability: InStock

R&D Systems | Catalog # FAB17781P

Submit a review

Citations (13)

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Hamster - Cricetulus (Chinese Hamster), Primate - Chlorocebus aethiops (African Green Monkey)

Applications

Validated:

Knockout Validated, Flow Cytometry

Cited:

Neutralization, Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG₁ Clone # P5D2

View all Integrin beta 1/CD29 Primary Antibodies »

Product Specifications

Immunogen

Human skin keratinocytes

Specificity

Detects human Integrin beta 1/CD29 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Integrin beta 1/CD29 PE‑conjugated Antibody

Detection of Integrin beta 1/CD29 in A549 human lung carcinoma cell line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta 1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Integrin beta 1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Integrin beta 1/CD29 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

Integrin beta 1/CD29 knockout A549 human lung carcinoma cell line was stained with Mouse Anti-Human Integrin beta 1/CD29 PE-conjugated Monoclonal Antibody (Catalog # FAB17781P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the Integrin beta 1/CD29 knockout A549 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin beta 1/CD29 PE‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

Knockout Validated

Integrin beta 1/CD29 is specifically detected in A549 lung carcinoma parental cell line but is not detectable in Integrin beta 1/CD29 knockout A549 cell line.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with at least ten different Integrin alpha subunits. It regulates not only its multiple ligands, but activates a signaling cascade in its expressing cells. CD29 is proposed to play a role in cell adhesion, apoptosis, and differentiation (1,2). Over amino acids (aa) 21-728, human and mouse share 92% aa sequence identity.