Human Integrin  beta 8 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB47752-100
MAB47752-SP
Detection of Integrin beta 8 in MG-63 Human Cell Line by Flow Cytometry.
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Human Integrin  beta 8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin  beta 8 in direct ELISAs
Source
Recombinant Monoclonal Rabbit IgG Clone # 2723C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese Hamster Ovary cell line CHO-derived human Integrin alpha V beta 8
Human Integrin alpha V (Phe31-Val992) and Human Integrin beta 8 (Glu43-Arg684)
Accession # NP_002201.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
MG‑63 human osteosarcoma cell line
Immunocytochemistry
3-25 µg/mL
Immersion fixed MG‑63 human osteosarcoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Integrin beta 8 in MG-63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Rabbit Anti-Human Integrin beta 8 Monoclonal Antibody (Catalog # MAB2723C, filled histogram) or Rabbit IgG Control Antibody (MAB1050,open histogram) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunocytochemistry View Larger

Integrin  beta 8 in MG‑63 Human Cell Line. Integrin  beta 8 was detected in immersion fixed MG‑63 human osteosarcoma cell line using Rabbit Anti-Human Integrin  beta 8 Monoclonal Antibody (Catalog # MAB47752) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 8

Integrin beta 8 (Integrin beta 8) is a 90 kDa type I transmembrane glycoprotein of the Integrin family of adhesion molecules. It associates with Integrin alpha V to form a receptor for vitronectin, fibrin, and the latency associated peptide (LAP). Binding to LAP promotes the proteolytic release of active TGF-beta from LAP. Integrin alpha V beta 8 is required for vascular morphogenesis in the embryonic brain and yolk sac. Within the extracellular domain, human Integrin beta 8 shares 87% aa sequence identity with mouse and rat Integrin beta 8.

Entrez Gene IDs
3696 (Human); 320910 (Mouse); 362800 (Rat)
Alternate Names
Integrin beta 8; integrin beta-8; integrin, beta 8; ITGB8

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