Integrin beta 8 (Integrin beta 8) is a 90 kDa type I transmembrane glycoprotein of the Integrin family of adhesion molecules. It associates with Integrin alpha V to form a receptor for vitronectin, fibrin, and the latency associated peptide (LAP). Binding to LAP promotes the proteolytic release of active TGF-beta from LAP. Integrin alpha V beta 8 is required for vascular morphogenesis in the embryonic brain and yolk sac. Within the extracellular domain, human Integrin beta 8 shares 87% aa sequence identity with mouse and rat Integrin beta 8.
Human Integrin beta 8 Antibody
R&D Systems | Catalog # MAB47752
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2723C
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Product Specifications
Immunogen
Chinese Hamster Ovary cell line CHO-derived human Integrin alpha V beta 8
Human Integrin alpha V (Phe31-Val992) and Human Integrin beta 8 (Glu43-Arg684)
Accession # NP_002201.1
Human Integrin alpha V (Phe31-Val992) and Human Integrin beta 8 (Glu43-Arg684)
Accession # NP_002201.1
Specificity
Detects human Integrin beta 8 in direct ELISAs
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Integrin beta 8 Antibody
Detection of Integrin beta 8 in MG-63 Human Cell Line by Flow Cytometry.
MG-63 human osteosarcoma cell line was stained with Rabbit Anti-Human Integrin beta 8 Monoclonal Antibody (Catalog # MAB2723C, filled histogram) or Rabbit IgG Control Antibody (MAB1050,open histogram) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-associated Proteins protocol.
Integrin beta 8 in MG‑63 Human Cell Line.
Integrin beta 8 was detected in immersion fixed MG‑63 human osteosarcoma cell line using Rabbit Anti-Human Integrin beta 8 Monoclonal Antibody (Catalog # MAB47752) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Applications for Human Integrin beta 8 Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: MG‑63 human osteosarcoma cell line
Sample: MG‑63 human osteosarcoma cell line
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed MG‑63 human osteosarcoma cell line
Sample: Immersion fixed MG‑63 human osteosarcoma cell line
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Integrin beta 8
Alternate Names
ITGB8
Gene Symbol
ITGB8
UniProt
Additional Integrin beta 8 Products
Product Documents for Human Integrin beta 8 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Integrin beta 8 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
