Human IRAK3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human IRAK3 in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IRAK3
    aa 1-596
    Accession # Q9Y616
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IRAK3 by Western Blot. Western blot shows lysates of EOL‑1 human acute myeloid (eosinophilic) leukemia cell line and THP‑1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRAK3 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IRAK3 by Western Blot. Western blot shows recombinant human IRAK1, recombinant human IRAK2, recombinant human IRAK3, and recombinant human IRAK4 (2 ng/lane). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRAK3

IRAK3 (Interleukin-1 receptor-associated kinase 3; also IRAK-M) is a 68‑70 kDa member of the Pelle subfamily, TKL Ser/Thr protein kinase family of proteins. IRAK3 has limited expression, being mainly found in macrophages, eosinophils and respiratory epithelium, including type II alveolar cells. IRAK3 is a negative regulator of TLR signaling. Following TLR activation, it appears to form a complex with MyD88, IRAK1 and IRAK4 on the TLR cytoplasmic domain. This effectively blocks IRAK1/4 phosphorylation and inhibits NF kappa B and MAPK downstream activation. Human IRAK3 is 596 amino acids (aa) in length, contains one death domain (aa 41‑106) and a nonfunctional protein kinase domain (aa 171‑443). There are six Ser phosphorylation sites. There is one splice variant with a deletion of aa 45‑105.

  • Long Name:
    Interleukin-1 Receptor-Associated Kinase 3
  • Entrez Gene IDs:
    11213 (Human); 73914 (Mouse); 314870 (Rat)
  • Alternate Names:
    ASRT5; EC 2.7.11.1; FLJ13601; IL-1 receptor-associated kinase M; interleukin-1 receptor-associated kinase 3; IRAK3; IRAK-3; IRAKM; IRAK-M
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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