Human IRAK3 Antibody

Detection of Human IRAK3 by Western Blot. Western blot shows lysates of EOL-1 human acute myeloid (eosinophilic) leukemia cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for IRAK3 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human IRAK3 by Western Blot. Western blot shows recombinant human IRAK1, recombinant human IRAK2, recombinant human IRAK3, and recombinant human IRAK4 (2 ng/lane). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1

Detection of IRAK3 by Western Blot IRAK3 isoform expression in primed macrophages. IRAK3 N-terminal to death domain (DD) (a.a. 1–14) is shaded grey, DD (15–104) is shaded blue, and pseudokinase domain (a.a. 171–446) is shaded green. (A) “Classical” full-length (CLS; NP_009130.2 a.a. 1–596). (B) “Alternative” IRAK-3 (ALT; NP_009130.2 a.a. 1–44, 105–596 of CLS) that lacks most of the DD. (C) Western blotting for IRAK3 in HEK293 cells (NIL) or HEK293 cells overexpressing classical or alternative IRAK3 blotted with two antibodies for IRAK3. (D) IRAK3 expression in monocyte-derived Mφ, unstimulated (NIL) or stimulated for 20 h with LPS, LPS/IFN gamma (M1), TNF, GM-CSF (GM), IL-4, or TGF-beta 1 (TGF); representative blots of two donors stained for IRAK3 (SAB3500193) and beta -actin (AC-74). Gene expression of (E) both IRAK3 isoforms and (F) the classical IRAK3 isoform in TNF-stimulated human Mφ. (G) Gene expression of Irak3 in LPS-stimulated (10 ng/mL) murine bone marrow-derived Mφ from wild-type or transgenic mice lacking either Tnfrsf1a or Tnfrsf1b; ANOVA for each respective knockout (KO) vs. wild type (WT), † p < 0.01, ‡ p < 0.001, n = 3–5 experiments. (H) Total IRAK3 gene expression in osteoarthritis (OA) and rheumatoid arthritis (RA) synovial explants and normal healthy monocytes (Mo), n = 12 OA, 19 RA, and 3 Mo. For (E, F, H) * p < 0.05, Student’s t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40370470), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human IRAK3 by Simple Western^TM. Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for IRAK3 at approximately 65 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.