Human IRAK3 Antibody

Catalog # Availability Size / Price Qty
AF6264
AF6264-SP
Detection of Human IRAK3 by Western Blot.
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Human IRAK3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IRAK3 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IRAK3
aa 1-596
Accession # Q9Y616
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human IRAK3 antibody by Western Blot. View Larger

Detection of Human IRAK3 by Western Blot. Western blot shows lysates of EOL-1 human acute myeloid (eosinophilic) leukemia cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRAK3 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human IRAK3 antibody by Western Blot. View Larger

Detection of Human IRAK3 by Western Blot. Western blot shows recombinant human IRAK1, recombinant human IRAK2, recombinant human IRAK3, and recombinant human IRAK4 (2 ng/lane). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRAK3

IRAK3 (Interleukin-1 receptor-associated kinase 3; also IRAK-M) is a 68‑70 kDa member of the Pelle subfamily, TKL Ser/Thr protein kinase family of proteins. IRAK3 has limited expression, being mainly found in macrophages, eosinophils and respiratory epithelium, including type II alveolar cells. IRAK3 is a negative regulator of TLR signaling. Following TLR activation, it appears to form a complex with MyD88, IRAK1 and IRAK4 on the TLR cytoplasmic domain. This effectively blocks IRAK1/4 phosphorylation and inhibits NF kappa B and MAPK downstream activation. Human IRAK3 is 596 amino acids (aa) in length, contains one death domain (aa 41‑106) and a nonfunctional protein kinase domain (aa 171‑443). There are six Ser phosphorylation sites. There is one splice variant with a deletion of aa 45‑105.

Long Name
Interleukin-1 Receptor-Associated Kinase 3
Entrez Gene IDs
11213 (Human); 73914 (Mouse); 314870 (Rat)
Alternate Names
ASRT5; EC 2.7.11.1; FLJ13601; IL-1 receptor-associated kinase M; interleukin-1 receptor-associated kinase 3; IRAK3; IRAK-3; IRAKM; IRAK-M

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