Human Irisin/FNDC5 Antibody

Catalog # Availability Size / Price Qty
Detection of Irisin/FNDC5 in CHO-S Transfected (Positive) and CHO-S Wild Type (Negative) Cells.
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Human Irisin/FNDC5 Antibody Summary

Detects Irisin peptide in direct ELISA.
Monoclonal Mouse IgG2A Clone # 1056111
Protein A or G purified from hybridoma culture supernatant
Synthetic peptide covering the C-term of the Irisin peptide sequences of the FNDC5 gene.

Accession # Q8NAU1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
Immersion fixed CHO-S Transfected (Positive) and CHO-S Wild Type (Negative) Cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Irisin/FNDC5 in CHO-S Transfected (Positive) and CHO-S Wild Type (Negative) Cells. Irisin/FNDC5 was detected in immersion fixed CHO-S Transfected (Positive) and CHO-S Wild Type (Negative) Cells using Mouse Anti-Human Irisin/FNDC5 Monoclonal Antibody (Catalog # MAB94201) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Irisin/FNDC5

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor  gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).

  1. Bostrom, P. et al. (2012) Nature 481:463.
  2. Novelle, M.G. et al. (2013) Int. J. Endocrinol. 2013:746281.
  3. Ferrer-Martinez, A. et al. (2002) Dev. Dyn. 224:154.
  4. Teufel, A. et al. (2002) Gene 297:79.
  5. Zhang, W. et al. (2015) Neurosci. Lett. 595:7.
  6. Roca-Rivada, A. et al. (2013) PLoS One 8:e60563.
  7. Vaughan, R.A. et al. (2014) Diabetes Obes. Metab. 16:711.
  8. Jeong Lee, H. et al. (2015) Mol. Endocrinol. 6:873.
  9. Elsen, M. et al. (2014) J. Endocrinol. 222:R25.
  10. Dun, S.L. et al. (2013) Neuroscience 240:155.
  11. Forouzanfar, M. et al. (2015) Cell. Biol. Int. 39:629.
  12. Hashemi, M.S. et al. (2013) Neuroscience 231:296.
  13. Moon, H.S. et al. (2013) Metabolism 62:1131.
  14. Colaianni, G. et al. (2014) Int. J. Endocrinol. 2014:902186.
Long Name
Fibronectin Type III Domain Containing 5
Entrez Gene IDs
252995 (Human); 384061 (Mouse); 260327 (Rat)
Alternate Names
fibronectin type III domain containing 5; fibronectin type III domain-containing protein 5; FNDC5; FRCP2; FRCP2Fibronectin type III repeat-containing protein 2; Irisin

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