|Detection of Human ITIH4 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ITIH4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8157) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ITIH4 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
The inter-alpha -trypsin inhibitor heavy chain (ITIH4), also known as IHRP (inter-alpha -trypsin heavy chain-related protein), PK-120 (plasma kallikrein-sensitive glycoprotein 120), or Gp120 (glycoprotein 120), is a secreted, 120 kDa, 930 amino acid (aa), N-glycosylated Type II acute phase protein. It is upregulated in response to trauma such as ischemic stroke or myocardial infarction, and functions as a trypsin inhibitor. It is activated by Kallikrein by cleavage between aa 688-689, creating 100 kDa and 35 kDa portions. Mature human ITIH4 shares 66% aa sequence identity with mouse and rat ITIH4. Human isoforms lacking aa 621-650 (914, 900 and 845 aa forms), aa 727-765 and 851-866 (845 aa) and having 14 aa inserted after aa 727 (914 aa) have been described. Proteolytic cleavage by plasma kallikrein produces a 100 kDa fragment that is further processed to 70 kDa, and 35 kDa fragments. An O-glycosylated form is reported in urine, but not in plasma.
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