Human JAM-C Antibody

Catalog # Availability Size / Price Qty
AF1189
AF1189-SP
Human JAM-C Antibody in Western Blot
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Citations (3)
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Human JAM-C Antibody Summary

Species Reactivity
Human
Specificity
Detects human JAM‑C in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 75% cross-reactivity with recombinant mouse (rm) JAM-C is observed and less than 1% cross-reactivity with recombinant human JAM-1 and rmJAM-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human JAM-C
Val32-Asn241 (Ala149Pro)
Accession # Q9BX67
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
Human platelets
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize JAM‑C-mediated adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line. Fong, S. et al. (2002) J. Immunol. 168:1618. The Neutralization Dose (ND50) is typically 2‑8 µg/mL in the presence of 0.2  µg/mL Recombinant Human JAM‑B Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human JAM‑C by Western Blot. View Larger

Detection of Human JAM‑C by Western Blot. Western blot shows lysates of human placenta tissue, human brain tissue, JAR human choriocarcinoma cell line, and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human JAM‑C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for JAM‑C at approximately 36-38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human JAM‑C by Simple Western<sup>TM</sup>. View Larger

Detection of Human JAM‑C by Simple WesternTM. Simple Western lane view shows lysates of JAR human choriocarcinoma cell line and human placenta, loaded at 0.2 mg/mL. A specific band was detected for JAM-C at approximately 53 and 54 kDa (as indicated) using 20 µg/mL of Goat Anti-Human JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Neutralization Cell Adhesion Mediated by JAM‑C and Neutralization by Human JAM‑C Antibody. View Larger

Cell Adhesion Mediated by JAM‑C and Neutralization by Human JAM‑C Antibody. Recombinant Human JAM-B/ VE-JAM Fc Chimera (Catalog # 1074-VJ), immobilized onto a microplate previously coated with Goat Anti-Human IgG Fc (Catalog # G-102-C), supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Human JAM-B Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Human JAM-C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1189). The ND50is typically 2-8 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-C

The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Human JAM-C cDNA predicts a 310 amino acid (aa) residue precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 46 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3, 4). Human JAM-C shares 86% aa sequence identity with its mouse homologue. It also shares approximately 36% and 32% aa sequence homology with human JAM-B and JAM-A, respectively (3‑5). Human JAM-C shows widespread tissue expression and the highest levels are found in the placenta, brain, kidney and heart. JAM-C is expressed on endothelial cells of high endothelial venules in human tonsil. It is also expressed on platelets, T-cells and NK cells (3‑5). Unlike other JAM family members, JAM-C forms only weak homotypic interactions. JAM-C binds to JAM-B to facilitate the interactions between JAM-B and the integrin alpha4beta1 (6). This heterotypic interaction between leukocyte JAM-C and endothelial JAM-B may play a role in regulating leukocyte transmigration (5). On platelets, JAM-C is a counter-receptor for the leukocyte integrin Mac-1(CD11b/CD18) (7). JAM-C has also been identified as a strong candidate gene for hypoplastic left heart syndrome (8).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B. Under this system, JAM-C refers to the protein encoded by the gene localized to human chromosome 11.

References
  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Arrate, M.P. et al. (2001) J. Biol. Chem. 276:45826.
  4. Liang, T. et al. (2002) J. Immunol. 168:1618.
  5. Johnson-Leger, C. et al. (2002) Blood 100:25793.
  6. Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
  7. Santoso, S. et al. (2002) J. Exp. Med. 196:679.
  8. Phillips, H.M. et al. (2002) Genomics 79:475.
Long Name
Junctional Adhesion Molecule C
Entrez Gene IDs
83700 (Human); 83964 (Mouse)
Alternate Names
CD323; JAM-2; JAM3; JAMC; JAM-C; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C

Product Datasheets

Citations for Human JAM-C Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin.
    Authors: Ludwig RJ, Hardt K, Hatting M, Bistrian R, Diehl S, Radeke HH, Podda M, Schon MP, Kaufmann R, Henschler R, Pfeilschifter JM, Santoso S, Boehncke WH
    Immunology, 2009;128(2):196-205.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Junctional adhesion molecule-C promotes metastatic potential of HT1080 human fibrosarcoma.
    Authors: Fuse C, Ishida Y, Hikita T, Asai T, Oku N
    J. Biol. Chem., 2007;282(11):8276-83.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. JAM-C is a component of desmosomes and a ligand for CD11b/CD18-mediated neutrophil transepithelial migration.
    Authors: Zen K, Babbin BA, Liu Y, Whelan JB, Nusrat A, Parkos CA
    Mol. Biol. Cell, 2004;15(8):3926-37.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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