Human Kallikrein 3/PSA Quantikine ELISA Kit

(11 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    0.069 ng/mL
  • Assay Range
    0.9 - 60 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human KLK3/PSA
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC23, Quantikine Immunoassay Control Group 8 - Please Inquire
Product Summary
The Quantikine Human KLK3/PSA Immunoassay is a 4.5 hour solid phase immunoassay designed to measure KLK3/PSA in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human KLK3/PSA, and antibodies raised against the recombinant protein. Natural human KLK3/PSA showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human KLK3/PSA.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.71.320.90.50.8

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.


The recovery of KLK3/PSA spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 93-105
EDTA Plasma (n=4) 100 93-103
Heparin Plasma (n=4) 101 97-109
Serum (n=4) 102 97-109
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of KLK3/PSA were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Kallikrein 3/PSA Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Kallikrein 3/PSA

The human tissue kallikrein (KLK) gene family contains 15 members that play important roles in cancer. Notably, kallikrein-1, also known as tissue kallikrein, cleaves kininogen to release the vasoactive kinin peptide, bradykinin or lysyl-bradykinin. Kallikrein-3, called prostate specific antigen (PSA), is an established tumor marker that aids in the diagnosis, staging, and follow up of prostate cancer. Kallikrein-4 is specifically expressed in the prostate and over-expressed in prostate cancer. Kallikrein-5 is widely expressed but found at high levels in skin, breast, brain and testis; over-expression is an indicator of poor prognosis in ovarian cancer. Kallikrein-8 is expressed in the brain and is a novel marker of ovarian and cervical cancer.

Human plasma kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma bound to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, coagulation factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase.

    • Entrez Gene IDs
      354 (Human);
    • Alternate Names
      APS seminin; EC 3.4.21; EC; Gamma-seminoprotein; hK3; Kallikrein 3; kallikrein 3, (prostate specific antigen); Kallikrein-3; kallikrein-related peptidase 3; KLK2A1; KLK3; P-30 antigen; prostate specific antigen; prostate-specific antigen; PSA semenogelase; PSA; Semenogelase; Seminin;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of the appropriate Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    11 Citations: Showing 1 - 10
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    Sample Type
    1. Targeted nanoparticles encapsulating (-)-epigallocatechin-3-gallate for prostate cancer prevention and therapy
      Authors: V Sanna, CK Singh, R Jashari, VM Adhami, JC Chamcheu, I Rady, M Sechi, H Mukhtar, IA Siddiqui
      Sci Rep, 2017;7(0):41573.
      Species: Mouse
      Sample Type: Serum
    2. Axitinib and crizotinib combination therapy inhibits bone loss in a mouse model of castration resistant prostate cancer.
      Authors: Eswaraka J, Giddabasappa A, Han G, Lalwani K, Eisele K, Feng Z, Affolter T, Christensen J, Li G
      BMC Cancer, 2014;14(0):742.
      Species: Mouse
      Sample Type: Serum
    3. Antitumor activity of a pyrrole-imidazole polyamide.
      Proc Natl Acad Sci U S A, 2013;110(5):1863-8.
      Species: Human
      Sample Type: Serum
    4. Metformin enhances the antiproliferative and apoptotic effect of bicalutamide in prostate cancer.
      Authors: Colquhoun, A J, Venier, N A, Vandersluis, A D, Besla, R, Sugar, L M, Kiss, A, Fleshner, N E, Pollak, M, Klotz, L H, Venkateswaran, V
      Prostate Cancer Prostatic Dis, 2012;15(4):346-52.
      Species: Mouse
      Sample Type: Serum
    5. Synergistic activity of the mTOR inhibitor ridaforolimus and the antiandrogen bicalutamide in prostate cancer models.
      Authors: Squillace RM, Miller D, Wardwell SD, Wang F, Clackson T, Rivera VM
      Int. J. Oncol., 2012;41(2):425-32.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer.
      Authors: Windus L, Kiss D, Glover T, Avery V
      Exp Cell Res, 2012;318(19):2507-19.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Sensitive plasma protein analysis by microparticle-based proximity ligation assays.
      Authors: Darmanis S, Nong RY, Hammond M, Gu J, Alderborn A, Vanelid J, Siegbahn A, Gustafsdottir S, Ericsson O, Landegren U, Kamali-Moghaddam M
      Mol. Cell Proteomics, 2010;9(2):327-35.
      Species: Human
      Sample Type: Plasma
    8. D,L-Sulforaphane causes transcriptional repression of androgen receptor in human prostate cancer cells.
      Authors: Kim SH, Singh SV
      Mol. Cancer Ther., 2009;8(7):1946-54.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Transcriptional repression and inhibition of nuclear translocation of androgen receptor by diallyl trisulfide in human prostate cancer cells.
      Authors: Stan SD, Singh SV
      Clin. Cancer Res., 2009;15(15):4895-903.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Urine analysis and protein networking identify met as a marker of metastatic prostate cancer.
      Authors: Russo AL, Jedlicka K, Wernick M, McNally D, Kirk M, Sproull M, Smith S, Shankavaram U, Kaushal A, Figg WD, Dahut W, Citrin D, Bottaro DP, Albert PS, Tofilon PJ, Camphausen K
      Clin. Cancer Res., 2009;15(13):4292-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Isosilybin B causes androgen receptor degradation in human prostate carcinoma cells via PI3K-Akt-Mdm2-mediated pathway.
      Authors: Deep G, Oberlies NH, Kroll DJ, Agarwal R
      Oncogene, 2008;27(28):3986-98.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all 11 Citations
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