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Human KAP1 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB7785U Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB7785U-100UG

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Human KAP1 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human KAP1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2b Clone # 821912
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human KAP1
Lys266-Ser624
Accession # Q13263
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: KAP1

KAP1 (KRAB-interacting protein 1; gene name TRIM28), also called TIF1b (transcription intermediary factor 1b) is an approximately 100 kDa nuclear corepressor that is a member of the TRIM (tripartite motif-containing) family. It is reported to bind both IRF7 and the SUMO enzyme and cause increased SUMOylation of IRF7. The 834 amino acid (aa) mature protein contains four zinc finger regions and a leucine zipper domain. A 752 aa human splice variant lacking aa 114-195 has been reported. Within the region used as an immunogen, human KAP1 shares 94% aa sequence identity with mouse and rat KAP1.

Long Name
KRAB-associated Protein 1
Entrez Gene IDs
10155 (Human); 21849 (Mouse)
Alternate Names
E3 SUMO-protein ligase TRIM28; EC 6.3.2.-; FLJ29029; KAP1; KAP-1; KAP1KRAB-associated protein 1; KRIP-1; Nuclear corepressor KAP-1; RNF96; RNF96KRAB-interacting protein 1; TF1B; TIF1B; TIF1-beta; TIF1BRING finger protein 96; transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; TRIM28; tripartite motif containing 28; tripartite motif-containing 28; Tripartite motif-containing protein 28

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