Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 821912
View all KAP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human KAP1
Lys266-Ser624
Accession # Q13263

Specificity

Detects human KAP1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human KAP1 Antibody

Detection of Human and Mouse KAP1 antibody by Western Blot.

Detection of Human and Mouse KAP1 by Western Blot.

Western blot shows lysates of PC-3 human prostate cancer cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for KAP1 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human KAP1 antibody by Simple WesternTM.

Detection of Human KAP1 by Simple WesternTM.

Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for KAP1 at approximately 117 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
KAP1 antibody in Neuro-2A Mouse Cell Line by Immunocytochemistry (ICC).

KAP1 in Neuro‑2A Mouse Cell Line.

KAP1 was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

KAP1 antibody in Human Liver Cancer Tissue by Immunohistochemistry (IHC-P).

KAP1 in Human Liver Cancer Tissue.

KAP1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of KAP1 in PC‑3 human prostate cancer cells

KAP1 was detected in immersion fixed PC‑3 human prostate cancer cells using Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Nuclear. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human KAP1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Neuro-2A mouse neuroblastoma cell line and PC‑3 human prostate cancer cells

Immunohistochemistry

0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue

Simple Western

2 µg/mL
Sample: PC‑3 human prostate cancer cell line

Western Blot

0.2 µg/mL
Sample: PC‑3 human prostate cancer cell line and Neuro‑2A mouse neuroblastoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB7785 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KAP1

KAP1 (KRAB-interacting protein 1; gene name TRIM28), also called TIF1b (transcription intermediary factor 1b) is an approximately 100 kDa nuclear corepressor that is a member of the TRIM (tripartite motif-containing) family. It is reported to bind both IRF7 and the SUMO enzyme and cause increased SUMOylation of IRF7. The 834 amino acid (aa) mature protein contains four zinc finger regions and a leucine zipper domain. A 752 aa human splice variant lacking aa 114-195 has been reported. Within the region used as an immunogen, human KAP1 shares 94% aa sequence identity with mouse and rat KAP1.

Long Name

KRAB-associated Protein 1

Alternate Names

KRIP-1, RNF96, TF1B, TIF1B, TRIM28

Entrez Gene IDs

10155 (Human); 21849 (Mouse)

Gene Symbol

TRIM28

UniProt

Q13263 (Human)

Additional KAP1 Products

Product Documents for Human KAP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human KAP1 Antibody

For research use only

Citations for Human KAP1 Antibody

Customer Reviews for Human KAP1 Antibody (1)

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  • Human KAP1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Human
    Verified Customer | Posted 02/09/2022
    Human KAP1 Antibody MAB7785

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Protocols

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