Human KAP1 Antibody

Catalog # Availability Size / Price Qty
MAB7785
MAB7785-SP
Detection of Human and Mouse KAP1 by Western Blot.
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Human KAP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human KAP1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 821912
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human KAP1
Lys266-Ser624
Accession # Q13263
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below
Immunohistochemistry
0.5-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse KAP1 antibody by Western Blot. View Larger

Detection of Human and Mouse KAP1 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for KAP1 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human KAP1 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human KAP1 by Simple WesternTM. Simple Western lane view shows lysates of PC‑3 human prostate cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for KAP1 at approximately 117 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry KAP1 antibody in Neuro-2A Mouse Cell Line by Immunocytochemistry (ICC). View Larger

KAP1 in Neuro‑2A Mouse Cell Line. KAP1 was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry KAP1 antibody in Human Liver Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

KAP1 in Human Liver Cancer Tissue. KAP1 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human KAP1 Monoclonal Antibody (Catalog # MAB7785) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KAP1

KAP1 (KRAB-interacting protein 1; gene name TRIM28), also called TIF1b (transcription intermediary factor 1b) is an approximately 100 kDa nuclear corepressor that is a member of the TRIM (tripartite motif-containing) family. It is reported to bind both IRF7 and the SUMO enzyme and cause increased SUMOylation of IRF7. The 834 amino acid (aa) mature protein contains four zinc finger regions and a leucine zipper domain. A 752 aa human splice variant lacking aa 114-195 has been reported. Within the region used as an immunogen, human KAP1 shares 94% aa sequence identity with mouse and rat KAP1.

Long Name
KRAB-associated Protein 1
Entrez Gene IDs
10155 (Human); 21849 (Mouse)
Alternate Names
E3 SUMO-protein ligase TRIM28; EC 6.3.2.-; FLJ29029; KAP1; KAP-1; KAP1KRAB-associated protein 1; KRIP-1; Nuclear corepressor KAP-1; RNF96; RNF96KRAB-interacting protein 1; TF1B; TIF1B; TIF1-beta; TIF1BRING finger protein 96; transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; TRIM28; tripartite motif containing 28; tripartite motif-containing 28; Tripartite motif-containing protein 28

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human KAP1 Antibody
By Anonymous on 02/09/2022
Application: IHC Sample Tested: Kidney tissue Species: Human