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Human KCC2/SLC12A5 APC-conjugated Antibody

Catalog #: FAB8369A Datasheet / COA / SDS

Discontinued Product

FAB8369A has been discontinued.
View all KCC2/SLC12A5 products.
Detection of KCC2/SLC12A5 in HEK293 Human Cell Line Transfected with Human KCC2/SLC12A5 and eGFP by Flow Cytometry.
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Human KCC2/SLC12A5 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human KCC2/SLC12A5 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # 905705
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human KCC2/SLC12A5
Accession # Q9H2X9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KCC2/SLC12A5 antibody in HEK293 Human Cell Line Transfected with Human KCC2/SLC12A5 and eGFP antibody by Flow Cytometry. View Larger

Detection of KCC2/SLC12A5 in HEK293 Human Cell Line Transfected with Human KCC2/SLC12A5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human KCC2/SLC12A5 and eGFP was stained with either (A) Mouse Anti-Human KCC2/SLC12A5 APC-conjugated Monoclonal Antibody (Catalog # FAB8369A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KCC2/SLC12A5

Solute Carrier, family 12 (potassium/chloride transporter), member 5 (SLC12A5) encodes KCC2, a multi-pass co-transporter for K+ and Cl-.  KCC2/SLC12A5 is expressed on neurons, and is dysregulated in schizophrenia, spasticity, and affective disorders.

Long Name
K-Cl Cotransporter 2
Entrez Gene IDs
57468 (Human); 57138 (Mouse); 171373 (Rat)
Alternate Names
Electroneutral potassium-chloride cotransporter 2; hKCC2; KCC2; KCC2K-Cl cotransporter 2; KIAA1176erythroid K-Cl cotransporter 2; Neuronal K-Cl cotransporter; SLC12A5; solute carrier family 12 (potassium/chloride transporter), member 5; solute carrier family 12 member 5

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SDS

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