Human KGF/FGF-7 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DKG00
Control Products Available
Human KGF/FGF-7 ELISA Cell Culture Supernate Standard Curve
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Product Details
Procedure
Citations (14)
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Human KGF/FGF-7 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
5.25 hours or 5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL)
Sensitivity
15 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human KGF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human KGF Immunoassay is a 5.25 or 5.5 hour solid phase ELISA designed to measure KGF in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human KGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant KGF accurately. Results obtained measuring natural human KGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human KGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 97.9 298 1229 95.5 294 1221
Standard Deviation 3.3 8.8 42.8 7.4 15.6 63.5
CV% 3.4 3 3.5 7.7 5.3 5.2

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 82.4 248 1031 86.5 266 1108
Standard Deviation 3.2 8.8 55.2 4.8 11.4 62
CV% 3.9 3.5 5.4 5.5 4.3 5.6

Recovery

The recovery of KGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 102 95-105
Citrate Plasma (n=5) 91 86-98
EDTA Plasma (n=5) 96 85-104
Heparin Plasma (n=5) 90 85-99
Serum (n=5) 93 85-100

Linearity

To assess the linearity of the assay, five samples were spiked with high concentrations of KGF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human KGF/FGF-7 ELISA Linearity

Data Examples

Human KGF/FGF-7 ELISA Cell Culture Supernate Standard Curve

Human KGF/FGF-7 ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: KGF/FGF-7

KGF (keratinocyte growth factor), also known as FGF-7, is induced in mesenchymal cells by inflammation or tissue damage. It functions as a paracrine factor during wound healing by inducing the proliferation of nearby epithelial cells. KGF plays a role in kidney morphogenesis, hair follicle development, and keratinocyte differentiation. It signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (IIIb)/KGF R.

Long Name:
Keratinocyte Growth Factor
Entrez Gene IDs:
2252 (Human); 403915 (Canine)
Alternate Names:
FGF7; FGF-7; fibroblast growth factor 7HBGF-7; HBGF7; HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor)
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well.
  10.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 1.75 hours.
  11.   Aspirate and wash 4 times.

  12. 200 µL Substrate Solution
  13.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human KGF/FGF-7 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Ex Vivo Expansion of Human Limbal Epithelial Cells Using Human Placenta-Derived and Umbilical Cord-Derived Mesenchymal Stem Cells
    Authors: SM Nam, YS Maeng, EK Kim, KY Seo, H Lew
    Stem Cells Int, 2017;2017(0):4206187.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Impact of Hyperglycemia and Low Oxygen Tension on Adipose-Derived Stem Cells Compared with Dermal Fibroblasts and Keratinocytes: Importance for Wound Healing in Type 2 Diabetes
    PLoS ONE, 2016;11(12):e0168058.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. The role of HGF/MET and FGF/FGFR in fibroblast-derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma.
    Authors: Saito S, Morishima K, Ui T, Hoshino H, Matsubara D, Ishikawa S, Aburatani H, Fukayama M, Hosoya Y, Sata N, Lefor A, Yasuda Y, Niki T
    BMC Cancer, 2015;15(0):82.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Mechanisms of renal phosphate loss in liver resection-associated hypophosphatemia.
    Authors: Nafidi O, Lapointe RW, Lepage R, Kumar R, D'Amour P
    Ann. Surg., 2009;249(5):824-7.
    Species: Human
    Sample Types: Serum
  5. Keratinocyte growth factor expression is suppressed in early acute lung injury/acute respiratory distress syndrome by smad and c-Abl pathways.
    Authors: Chandel NS, Budinger GR, Mutlu GM, Varga J, Synenki L, Donnelly HK, Zirk A, Eisenbart J, Jovanovic B, Jain M
    Crit. Care Med., 2009;37(5):1678-84.
    Species: Human
    Sample Types: BALF
  6. Prevention of radiation-induced oral mucositis after adenoviral vector-mediated transfer of the keratinocyte growth factor cDNA to mouse submandibular glands.
    Authors: Zheng C, Cotrim AP, Sunshine AN, Sugito T, Liu L, Sowers A, Mitchell JB, Baum BJ
    Clin. Cancer Res., 2009;15(14):4641-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Tissue engineering for full-thickness burns: a dermal substitute from bench to bedside.
    Authors: Kellouche S, Martin C, Korb G, Rezzonico R, Bouard D, Benbunan M, Dubertret L, Soler C, Legrand C, Dosquet C
    Biochem. Biophys. Res. Commun., 2007;363(3):472-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
    Authors: Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K
    Int. J. Cancer, 2007;120(3):686-93.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Keratinocyte-fibroblast paracrine interaction: the effects of substrate and culture condition.
    Authors: Witte RP, Kao WJ
    Biomaterials, 2005;26(17):3673-82.
    Species: Human
    Sample Types: Whole Cells
  10. Salivary leptin induces increased expression of growth factors in oral keratinocytes.
    Authors: Groschl M, Topf HG, Kratzsch J, Dotsch J, Rascher W, Rauh M
    J. Mol. Endocrinol., 2005;34(2):353-66.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. High concentrations of keratinocyte growth factor in airways of premature infants predicted absence of bronchopulmonary dysplasia.
    Authors: Danan C, Franco ML, Jarreau PH, Dassieu G, Chailley-Heu B, Bourbon J, Delacourt C
    Am. J. Respir. Crit. Care Med., 2002;165(10):1384-7.
    Species: Human
    Sample Types: Tracheal Lavage Fluid
  12. Increase in gap junctional intercellular communication by high molecular weight hyaluronic acid associated with fibroblast growth factor 2 and keratinocyte growth factor production in normal human dermal fibroblasts.
    Authors: Park JU, Tsuchiya T
    Tissue Eng., 2002;8(3):419-27.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Upregulation of keratinocyte growth factor in the tracheal ligation lamb model of congenital diaphragmatic hernia.
    Authors: McCabe AJ, Carlino U, Holm BA, Glick PL
    J. Pediatr. Surg., 2001;36(1):128-32.
    Species: Ovine
    Sample Types: Tissue Homogenates
  14. c-Jun and JunB antagonistically control cytokine-regulated mesenchymal-epidermal interaction in skin.
    Authors: Szabowski A, Maas-Szabowski N, Andrecht S, Kolbus A, Schorpp-Kistner M, Fusenig NE, Angel P
    Cell, 2000;103(5):745-55.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human KGF/FGF-7 Quantikine ELISA Kit
By Anonymous on 04/05/2018
Application: Sample Tested: Cell culture supernatant