Human Kidney Biomarker Array Kit


R&D Systems Proteome Profiler™ Human Kidney Biomarker Array Kit (Catalog # ARY019) is a rapid, sensitive, and economical tool to simultaneously detect the relative changes of 38 proteins in a single sample.  No specialized equipment is necessary.

Simultaneously detect the levels of these cell stress-related proteins in a single sample.
Adiponectin Fetuin A Renin
Aminopeptidase N GRO-alpha/CXCL1 Resistin
Angiotensinogen/Serpin A8 IL-1ra SCF
Annexin V IL-6 Serpin A3
beta2-Microglobulin IL-10 TNF-alpha
Clusterin TIM-1/KIM-1/HAVCR TNF RI
CXCL16 Lipocalin-2/NGAL Trefoil Factor 3
Cyr61/CCN MCP-1/CCL2 Thrombospondin-1
Cystatin C MMP-9 TWEAK
DPPIV/CD26 Neprilysin uPA

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Urine, cell culture supernatant, and cell or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any biomarker/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of kidney biomarker bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.