R&D Systems Proteome Profiler™ Human Kidney Biomarker Array Kit (Catalog # ARY019) is a rapid, sensitive, and economical tool to simultaneously detect the relative changes of 38 proteins in a single sample. No specialized equipment is necessary.
Simultaneously detect the levels of these cell stress-related proteins in a single sample.
Trefoil Factor 3
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Urine, cell culture supernatant, and cell or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any biomarker/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of kidney biomarker bound. Chemiluminescence is detected in the same manner as a Western blot.
4 Array Membranes
Detection Antibody Cocktail
Chemiluminescent Detection Reagents
Transparency Overlay Template
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.