Human LAG-3 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB23193A
Detection of LAG‑3 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (4)
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Human LAG-3 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LAG-3 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 874501
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAG-3
Leu23-Leu450
Accession # P18627
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LAG‑3 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of LAG‑3 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 1 µg/mL PHA for 5 days were stained with Mouse Anti-Human LAG-3 APC-conjugated Monoclonal Antibody (Catalog # FAB23193A) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of LAG-3 antibody in HEK293 Human Cell Line Transfected with Human LAG-3 and eGFP antibody by Flow Cytometry. View Larger

Detection of LAG-3 in HEK293 Human Cell Line Transfected with Human LAG-3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human LAG-3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LAG-3 APC-conjugated Monoclonal Antibody (Catalog # FAB23193A). Quadrant markers were set based on control antibody staining (Catalog # IC002A, data not shown). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LAG-3

LAG-3 (Lymphocyte activation gene-3), also known as CD223, is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 and CD4 molecules share < 20% aa sequence homology but have a similar structure (1, 2). Both molecules bind to MHC class II. LAG-3 binds to MHC class II with higher affinity compared to CD4. Both LAG-3 and CD4 genes are located on the distal part of the short arm of chromosome 12.

LAG-3 is an activation-induced molecule, expressed on activated T cells and NK cells, but not on resting T cells. Studies using LAG-3 -/- mice have shown significant delay of T cell apoptosis following antigen stimulation and increased size of memory T cells pool following infection (3, 4). It also has been reported that anti-LAG-3 antibodies up-regulate T cell activation by blocking interaction of LAG-3 and MHC class II. The study has demonstrated that LAG-3 is selectively expressed on activated CD4+CD25+ TReg cells and plays a role in their suppressive activity (5). This evidence indicated, unlike the interaction of CD4 with MHC class II that plays a positive role in T cell activation, LAG-3 binds to MHC class II and negatively regulates T cell activation through LAG-3 signaling. On the other hand, studies have shown that binding of LAG-3 to MHC class II molecules on antigen presenting cells induce maturation of dendritic cells and cytokine secretion by monocytes through MHC class II signal transduction (6). Taken together, LAG-3 may have two major functions, it negatively regulates T cells activation through LAG-3 signaling and stimulates antigen presenting cells which express MHC class II.

References
  1. Triebel, F. et al. (1990) J. Exp. Med. 171:1393.
  2. Baixeras, E. et al. (1992) J. Exp. Med 176:327.
  3. Workman, C.J. and D.A. Vignali (2003) Eur. J. Immunol. 33:970.
  4. Workman, C.J. et al. (2004) J. Immunol. 172:5450.
  5. Huang, C.T. et al. (2004) Immunity 21:503.
  6. Andreae, S. et al. (2003) Blood 102:2130.
Long Name
Lymphocyte-activation Gene 3
Entrez Gene IDs
3902 (Human); 16768 (Mouse); 297596 (Rat); 102122272 (Cynomolgus Monkey)
Alternate Names
CD223 antigen; CD223; LAG3; LAG-3; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG-3

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Citations for Human LAG-3 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Cerebrospinal fluid CD4+ T cell infection in humans and macaques during acute HIV-1 and SHIV infection
    Authors: V Sharma, M Creegan, A Tokarev, D Hsu, BM Slike, C Sacdalan, P Chan, S Spudich, J Ananworani, MA Eller, SJ Krebs, S Vasan, DL Bolton, RV254/SEAR
    PloS Pathogens, 2021-12-07;17(12):e1010105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Plasmacytoid dendritic cells have divergent effects on HIV infection of initial target cells and induce a pro-retention phenotype
    Authors: O Tong, G Duette, TR O'Neil, CM Royle, H Rana, B Johnson, N Popovic, S Dervish, MAE Brouwer, H Baharlou, E Patrick, G Ctercteko, S Palmer, E Lee, E Hunter, AN Harman, AL Cunningham, N Nasr
    PloS Pathogens, 2021-04-19;17(4):e1009522.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Enhancing the potency and specificity of engineered T cells for cancer treatment
    Authors: S Sukumaran, N Watanabe, P Bajgain, K Raja, S Mohammed, WE Fisher, MK Brenner, AM Leen, JF Vera
    Cancer Discov, 2018-06-07;0(0):.
    Species: Human
    Sample Types: Whole Cells
  4. Diagnostic value of peripheral blood immune profiling in colorectal cancer.
    Authors: Choi J, Maeng H, Lee S, Kim Y, Kim D, Lee H, Namgung J, Oh H, Kim T, Jeong J, Park S, Choi Y, Kang Y, Yoon S, Lee J
    Ann Surg Treat Res, 2018-05-29;94(6):312-321.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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