Human LAMP-1/CD107a Fluorescein-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LAMP-1/CD107a in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 508921
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LAMP-1/CD107a
    Ala28-Asn380
    Accession # P11279
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LAMP‑1/CD107a in Human NK cells by Flow Cytometry. Human NK Cells either (A) stimulated in a 1:1 ratio with K562 human chronic myelogenous leukemia cell line and 3 μM monensin for 5 hours or (B) unstimulated were stained with Mouse Anti-Human LAMP‑1/CD107a Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC4800F) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.
Intracellular Staining by Flow Cytometry
Detection of LAMP-1/CD107a in THP-1 Human Cell Line by Flow Cytometry.
THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human LAMP-1/CD107a Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC4800F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LAMP-1/CD107a
Lysosome-associated Membrane Protein-1 (LAMP-1), also known as CD107a, is a 100-130 kDa member of the LAMP family of glycoproteins. It is expressed in lysosomal and plasma membranes of macrophages, NK and T cells, and with LAMP-2, is essential for the formation of phagolysosomes. On the cell surface, it also presents carbohydrates to selectins. Mature human LAMP-1 is a 389 amino acid (aa) type I transmembrane glycoprotein. It contains a 354 aa luminal/extracellular domain (ECD) (aa 28-381) and a 12 aa cytoplasmic tail (aa 405-416). The ECD has two large looping regions (aa 28-193 and 227-381) plus multiple N- and O-linked glycosylation sites. There is one reported splice variant that shows a deletion of aa 135-187, and two potential isoform variants that show equal number aa substitutions within the first 40 aa of the molecule's ORF. Over aa 28-380, human LAMP-1 shares 64% aa identity with mouse LAMP-1.
  • Long Name:
    Lysosome-associated Membrane Glycoprotein 1
  • Entrez Gene IDs:
    3916 (Human); 16783 (Mouse)
  • Alternate Names:
    CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 6  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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