Human LAMP2/CD107b Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Conjugated LAMP2/CD107b antibodies are ideal for immunocytochemistry colocalization studies in lysosomes. The unconjugated antibody detects human LAMP2/CD107b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LAMP1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 743320
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LAMP2/CD107b
    Leu29-Phe375
    Accession # P13473
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
LAMP2/CD107b in HeLa Human Cell Line. LAMP2/CD107b was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LAMP2/CD107b Biotinylated Monoclonal Antibody (Catalog # BAM6228) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (orange; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAMP-2/CD107b
Lysosomal associated membrane protein 2 (LAMP2), also known as CD107b and LGP110, is an approximately 110 kDa transmembrane glycoprotein that is a major component of lysosomal membranes (1). Mature human LAMP2 consists of a 347 amino acid (aa) intralumenal domain, a 24 aa transmembrane segment, and a 35 aa cytoplasmic tail (2). Its lumenal domain is organized into two heavily N-glycosylated regions separated by a Ser/Pro-rich linker that carries a minor amount of O‑linked glycosylation (2, 3). Alternate splicing generates a human LAMP2 isoform (LAMP2B) with a substituted juxtamembrane lumenal region, transmembrane segment, and cytoplasmic tail (4). Within the lumenal domain, human LAMP2 shares approximately 64% aa sequence identity with mouse and rat LAMP2. LAMP2 itself is subject to lysosomal degradation following cleavage of its lumenal domain (5). It mediates the lysosomal uptake of the chaperone HSC73 in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles (6, 7). In cytotoxic T cells and mast cells, LAMP2 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine (8-10). Up‑regulated LAMP2 at the plasma membrane serves as an indicator of cell activation of CD8+ T cells, mast cells, monocytes, and platelets (9-12). LAMP2 is a native ligand for lectins Galectin-1 and Galectin-3 (13‑15).
  • References:
    1. Eskelinen, E.-L. et al. (2003) Trends Cell Biol. 13:137.
    2. Fukuda, M. et al. (1988) J. Biol. Chem. 263:18920.
    3. Carlsson, S.R. et al. (1988) J. Biol. Chem. 263:18911.
    4. Konecki, D.S. et al. (1995) Biochem. Biophys. Res. Commun. 215:757.
    5. Cuervo, A.M. and J.F. Dice (2000) Traffic 1:570.
    6. Cuervo, A.M. and J.F. Dice (1996) Science 273:501.
    7. Tanaka, Y. et al. (1990) Nature 406:902.
    8. Peters, P.J. et al. (1991) J. Exp. Med. 173:1099.
    9. Betts, M.R. et al. (2003) J. Immunol. Meth. 281:65.
    10. Grutzkau, A. et al. (2004) Cytometry 61:62.
    11. Kannan, K. et al. (1996) Cell. Immunol. 171:10.
    12. Silverstein, R.L. and M. Febbraio (1992) Blood 80:1470.
    13. Skrincosky, D.M. et al. (1993) Cancer Res. 53:2667.
    14. Inohara, H. and Raz, A. (1994) Biochem. Biophys. Res. Commun. 201:1366.
    15. Ohannesian D.W. et al. (1994) Cancer Res. 54:5992.
  • Long Name:
    Lysosome-associated Membrane Glycoprotein 2
  • Entrez Gene IDs:
    3920 (Human)
  • Alternate Names:
    CD107 antigen-like family member B; CD107b antigen; CD107b; LAMP2; LAMP-2; LAMPB; LGP110; lysosomal-associated membrane protein 2; lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2
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