Human LAMP-2/CD107b Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Conjugated LAMP2/CD107b antibodies are ideal for immunocytochemistry colocalization studies in lysosomes. The unconjugated antibody detects human LAMP2/CD107b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LAMP1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 743320
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LAMP2/CD107b
    Leu29-Phe375
    Accession # P13473
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry

LAMP2/CD107b in HeLa Human Cell Line. LAMP2/CD107b was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human LAMP2/CD107b Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC6228G) at 25 μg/mL dilution overnight at 4° C and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry

Detection of LAMP2/CD107b in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human LAMP2/CD107b Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC6228G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LAMP-2/CD107b
Lysosomal associated membrane protein 2 (LAMP2), also known as CD107b and LGP110, is an approximately 110 kDa transmembrane glycoprotein that is a major component of lysosomal membranes (1). Mature human LAMP2 consists of a 347 amino acid (aa) intralumenal domain, a 24 aa transmembrane segment, and a 35 aa cytoplasmic tail (2). Its lumenal domain is organized into two heavily N-glycosylated regions separated by a Ser/Pro-rich linker that carries a minor amount of O‑linked glycosylation (2, 3). Alternate splicing generates a human LAMP2 isoform (LAMP2B) with a substituted juxtamembrane lumenal region, transmembrane segment, and cytoplasmic tail (4). Within the lumenal domain, human LAMP2 shares approximately 64% aa sequence identity with mouse and rat LAMP2. LAMP2 itself is subject to lysosomal degradation following cleavage of its lumenal domain (5). It mediates the lysosomal uptake of the chaperone HSC73 in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles (6, 7). In cytotoxic T cells and mast cells, LAMP2 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine (8-10). Up‑regulated LAMP2 at the plasma membrane serves as an indicator of cell activation of CD8+ T cells, mast cells, monocytes, and platelets (9-12). LAMP2 is a native ligand for lectins Galectin-1 and Galectin-3 (13‑15).
  • References:
    1. Eskelinen, E.-L. et al. (2003) Trends Cell Biol. 13:137.
    2. Fukuda, M. et al. (1988) J. Biol. Chem. 263:18920.
    3. Carlsson, S.R. et al. (1988) J. Biol. Chem. 263:18911.
    4. Konecki, D.S. et al. (1995) Biochem. Biophys. Res. Commun. 215:757.
    5. Cuervo, A.M. and J.F. Dice (2000) Traffic 1:570.
    6. Cuervo, A.M. and J.F. Dice (1996) Science 273:501.
    7. Tanaka, Y. et al. (1990) Nature 406:902.
    8. Peters, P.J. et al. (1991) J. Exp. Med. 173:1099.
    9. Betts, M.R. et al. (2003) J. Immunol. Meth. 281:65.
    10. Grutzkau, A. et al. (2004) Cytometry 61:62.
    11. Kannan, K. et al. (1996) Cell. Immunol. 171:10.
    12. Silverstein, R.L. and M. Febbraio (1992) Blood 80:1470.
    13. Skrincosky, D.M. et al. (1993) Cancer Res. 53:2667.
    14. Inohara, H. and Raz, A. (1994) Biochem. Biophys. Res. Commun. 201:1366.
    15. Ohannesian D.W. et al. (1994) Cancer Res. 54:5992.
  • Long Name:
    Lysosome-associated Membrane Glycoprotein 2
  • Entrez Gene IDs:
    3920 (Human)
  • Alternate Names:
    CD107 antigen-like family member B; CD107b antigen; CD107b; LAMP2; LAMP-2; LAMPB; LGP110; lysosomal-associated membrane protein 2; lysosome-associated membrane glycoprotein 2; Lysosome-associated membrane protein 2
Related Research Areas
Isotype Controls
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Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 3  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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