Human LAP (TGF-beta 1) Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human LAP in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 27235
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    3-25 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize LAP TGF‑ beta 1 inhibition of TGF‑ beta 1 growth inhibition in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 500 ng/mL Recombinant Human LAP TGF‑ beta 1 and 1 ng/mL Recombinant Human TGF‑ beta 1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
LAP (TGF-beta 1) in HEK293 Human Cell Line. LAP (TGF-beta 1) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB246) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
LAP TGF‑ beta 1 Inhibition of TGF‑ beta 1 Activity and Neutralization by Human LAP TGF‑ beta 1 Antibody. Recombinant Human LAP TGF‑ beta 1 (Catalog # 246-LP) inhibits Recombinant Human TGF‑ beta 1 (Catalog # 240-B) growth inhibition activity in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF‑ beta 1 (1 ng/mL) activity elicited by Recombinant Human LAP TGF‑ beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human LAP TGF‑ beta 1 Monoclonal Antibody (Catalog # MAB246). The ND50 is typically 1-5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAP (TGF-beta 1)
The TGF-beta family includes several related proteins (70-80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF-beta 1, TGF‑ beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4, and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF-beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.
  • Long Name:
    Latency-associated Peptide
  • Entrez Gene IDs:
    7040 (Human)
  • Alternate Names:
    LAP (TGFbeta 1); LAP (TGF-beta 1)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Induction of latency-associated peptide (transforming growth factor-beta(1)) expression on CD4+ T cells reduces Toll-like receptor 4 ligand-induced tumour necrosis factor-alpha production in a transforming growth factor-beta-dependent manner.
    Authors: Boswell S, Sharif S, Alisa A, Pereira SP, Williams R, Behboudi S
    Immunology, 2011;133(3):278-87.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2005;105(2):750-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Neut
  3. Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression.
    Authors: Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G
    Eur. J. Immunol., 2004;34(12):3485-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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