Human LAP (TGF-beta 1) Antibody (27235)

R&D Systems | Catalog # MAB246

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat, Complex Species Category, Virus - HIV-1

Applications

Validated:

Neutralization, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Multiplex Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 27235
View all LAP (TGF-beta 1) Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)

Specificity

Detects human LAP in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human LAP (TGF-beta 1) Antibody (27235)

LAP (TGF-beta 1) antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

LAP (TGF-beta 1) in HEK293 Human Cell Line.

LAP (TGF-beta 1) was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human LAP (TGF-beta 1) Monoclonal Antibody (Catalog # MAB246) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
LAP TGF‑ beta 1 Inhibition of TGF‑ beta 1 Activity and Neutralization by Human LAP TGF‑ beta 1 Antibody.

LAP TGF‑ beta 1 Inhibition of TGF‑ beta 1 Activity and Neutralization by Human LAP TGF‑ beta 1 Antibody.

Recombinant Human LAP TGF-beta 1 (Catalog # 246-LP) inhibits Recombinant Human TGF-beta 1 (Catalog # 240-B) growth inhibition activity in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human TGF-beta 1 (1 ng/mL) activity elicited by Recombinant Human LAP TGF-beta 1 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human LAP TGF-beta 1 Monoclonal Antibody (Catalog # MAB246). The ND50 is typically 1-5 µg/mL.
Detection of LAP (TGF-beta 1) by Western Blot

Detection of LAP (TGF-beta 1) by Western Blot

Specificity of R58 and L59 antibodies. (a) Schematic structure of a GST- rhLTGF-beta 1 protein generated in bacteria and a commercial rhLAP beta 1 generated in insect cells. The PLK cleavage site is indicated by arrowhead. (b-e) GST-LTGF-beta 1/rhLAP beta 1was incubated with/without PLK or PLN for 45 min at 37°C, and thereafter equal amounts of samples containing either 50 ng GST-rhLTGF-beta 1 or 500 ng rhLAP beta 1 were subjected to each lane in SDS-PAGE under reducing conditions followed by Western blot analyses using R58/L59 antibodies (b, d, respectively). Membranes used in panels b and d were reprobed with monoclonal (c) or polyclonal (e) anti-LAP antibodies. Lanes 1, intact GST-rhLTGF-beta 1 or rhLAP-beta 1; lanes 2, PLK digested GST-rhLTGF-beta 1 or rhLAP-beta 1; lanes 3, PLK; lane 4, PLN digested GST-rhLTGF-beta 1 or rhLAP-beta 1; lane 5, PLN. Representative results from three independent experiments with a similar result are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24877031), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human LAP (TGF-beta 1) Antibody (27235)

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Neutralization

Measured by its ability to neutralize LAP TGF‑ beta 1 inhibition of TGF‑ beta 1 growth inhibition in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 500 ng/mL Recombinant Human LAP TGF‑ beta 1 and 1 ng/mL Recombinant Human TGF‑ beta 1.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LAP (TGF-beta 1)

The TGF-beta family includes several related proteins (70-80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF-beta 1, TGF‑ beta 2, TGF-beta 1.2, TGF-beta 3, TGF-beta 4, and TGF-beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF-beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF-beta family members are naturally associated with their own distinct LAPs.

Long Name

Latency-associated Peptide

Alternate Names

LAP (TGFbeta 1)

Entrez Gene IDs

7040 (Human)

Gene Symbol

TGFB1

Additional LAP (TGF-beta 1) Products

Product Documents for Human LAP (TGF-beta 1) Antibody (27235)

Certificate of Analysis

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Product Specific Notices for Human LAP (TGF-beta 1) Antibody (27235)

For research use only

Citations for Human LAP (TGF-beta 1) Antibody (27235)

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