Human LAT Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB63341R-100UG
R&D Systems Antibodies
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Human LAT Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human LAT in direct ELISAs.
Source
Monoclonal Mouse IgG2b Clone # 661002
Immunogen
E. coli-derived recombinant human LAT
His30-Gln113
Accession # O43561
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood lymphocytes treated with Recombinant Human IL-4 (Catalog # 204-IL) and Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LAT

LAT (Linker for activation of T cells; also pp36) is a 36-38 kDa type III (no signal sequence; extracellular N-terminus) transmembrane adaptor phosphoprotein. It is expressed on mast cells, NK cells, monocytes (but not macrophages), platelets and T cells. LAT is phosphorylated upon ligation of the TCR, GPVI, and Fc epsilon RI, and serves as an anchor for several SH2-domain containing signaling proteins, including PLCg1, SLP76 and Grb2. Human LAT is 262 amino acids (aa) in length. It contains a four aa extracellular segment with a 235 aa cytoplasmic domain (aa 28-262). Palmitoylation occurs on Cys26 and 29. There are 13 Ser/Thr phosphorylation sites plus five Tyr sites. Multiple potential splice variants are reported. There is an alternative start site 36 aa upstream of the standard site that may be accompanied by a deletion of aa 114-142. There is also a deletion of aa 88-97, and three substitutions: two aa for aa 215-262, 38 aa for aa 55-262, and 50 aa for aa 35-262. Over aa 30-113, human LAT shares 73% aa identity with mouse LAT.  

Long Name
Linker for Activation of T cells
Entrez Gene IDs
27040 (Human); 16797 (Mouse); 81511 (Rat)
Alternate Names
36 kDa phospho-tyrosine adapter protein; LAT; LAT1,36 kDa phospho-tyrosine adaptor protein; linker for activation of T cells; linker for activation of T-cells family member 1; p36-38; pp36

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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