Human LDL R Antibody

(11 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human LDL R in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse LDL R is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human LDL R
    Asp193-Arg788
    Accession # P01130
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human LDL R (Catalog # 2148-LD)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Human LDL R (Catalog # 2148-LD) to immobilized human low density lipoprotein coated at 2 µg/mL (100 µL/well). At 5 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry

LDL R in Human Liver. LDL R was detected in formalin fixed paraffin-embedded sections of human liver using Goat Anti-Human LDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2148) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LDL R

The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors. This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell‑surface molecules, expanding their range of activities. Human LDL R is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail. The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr‑rich region that serves as a carbohydrate attachment point. There is extensive O‑linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120 ‑ 160 kDa. Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits. The extracellular region of human LDL R is 51% aa identical to the extracellular region of human VLDL R, and 79% aa identical to the extracellular region of mouse LDL R. LDL R is constitutively expressed and binds apoB of LDL and apoE of VLDL. It is responsible for clearing 70% of plasma LDL in liver. Mutations in the LDL R gene cause the autosomal dominant disorder, familial hypercholesterolemia.

  • Long Name:
    Low Density Lipoprotein Receptor
  • Entrez Gene IDs:
    3949 (Human); 16835 (Mouse); 300438 (Rat)
  • Alternate Names:
    FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Applications
Sample Type
  1. Applying antibody-sensitive hypervariable region 1-deleted hepatitis C virus to the study of escape pathways of neutralizing human monoclonal antibody AR5A
    Authors: R Velázquez-, M Law, J Bukh, J Prentoe
    PLoS Pathog, 2017;13(2):e1006214.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Identification of a Chrysanthemic Ester as an Apolipoprotein E Inducer in Astrocytes
    PLoS ONE, 2016;11(9):e0162384.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Alirocumab, a Therapeutic Human Antibody to PCSK9, Does Not Affect CD81 Levels or Hepatitis C Virus Entry and Replication into Hepatocytes
    PLoS ONE, 2016;11(4):e0154498.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation
    Authors: Elodie Weider
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Common Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Epitopes Mediate Multiple Routes for Internalization and Function.
    Authors: DeVay R, Yamamoto L, Shelton D, Liang H
    PLoS ONE, 2015;10(4):e0125127.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Plasma Membrane Tetraspanin CD81 Complexes with Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR), and Its Levels Are Reduced by PCSK9.
    Authors: Le Q, Blanchet M, Seidah N, Labonte P
    J Biol Chem, 2015;290(38):23385-400.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  7. Differential complement activation pathways promote C3b deposition on native and acetylated LDL thereby inducing lipoprotein binding to the complement receptor 1.
    Authors: Klop B, van der Pol P, van Bruggen R, Wang Y, de Vries M, van Santen S, O'Flynn J, van de Geijn G, Njo T, Janssen H, de Man P, Jukema J, Rabelink T, Rensen P, van Kooten C, Cabezas M
    J Biol Chem, 2014;289(51):35421-30.
    Species: Hamster
    Sample Type: Whole Cells
    Application: Blocking
  8. Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
    Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
    J Biol Chem, 2013;288(15):10805-18.
  9. Hormonal modulators of glial ABCA1 and apoE levels.
    Authors: Fan, Jianjia, Shimizu, Yoko, Chan, Jeniffer, Wilkinson, Anna, Ito, Ayaka, Tontonoz, Peter, Dullaghan, Edie, Galea, Liisa A, Pfeifer, Tom, Wellington, Cheryl L
    J Lipid Res, 2013;54(11):3139-50.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. The proprotein convertase PC7: unique zymogen activation and trafficking pathways.
    Authors: Rousselet E, Benjannet S, Hamelin J, Canuel M, Seidah NG
    J. Biol. Chem., 2011;286(4):2728-38.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Dissection of the endogenous cellular pathways of PCSK9-induced low density lipoprotein receptor degradation: evidence for an intracellular route.
    Authors: Poirier S, Mayer G, Poupon V, McPherson PS, Desjardins R, Ly K, Asselin MC, Day R, Duclos FJ, Witmer M, Parker R, Prat A, Seidah NG
    J. Biol. Chem., 2009;284(42):28856-64.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 11 Citations
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Isotype Controls
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Normal Goat IgG Control

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Secondary Antibodies
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 application: Flow Cytometry.

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Summary

ApplicationFlow Cytometry
Sample TestedHEK293 human embryonic kidney cell line
SpeciesHuman

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