Human LDL R Antibody

Catalog # Availability Size / Price Qty
AF2148
AF2148-SP
LDL R  in HepG2 Human Cell Line.
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Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (1)

Human LDL R Antibody Summary

Species Reactivity
Human
Specificity
Detects human LDL R in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse LDL R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human LDL R
Asp193-Arg788
Accession # P01130
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human LDL R (Catalog # 2148-LD)
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.2-0.6 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Human LDL R (Catalog # 2148-LD) to immobilized human low density lipoprotein coated at 2 µg/mL (100 µL/well). At 5 μg/mL, this antibody will block >90% of the binding.
 
Immunocytochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry LDL R  in HepG2 Human Cell Line. View Larger

LDL R in HepG2 Human Cell Line. LDL R was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human LDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2148) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry LDL R in Human Liver. View Larger

LDL R in Human Liver. LDL R was detected in formalin fixed paraffin-embedded sections of human liver using Goat Anti-Human LDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2148) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LDL R

The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors. This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell‑surface molecules, expanding their range of activities. Human LDL R is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail. The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr‑rich region that serves as a carbohydrate attachment point. There is extensive O‑linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120 ‑ 160 kDa. Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits. The extracellular region of human LDL R is 51% aa identical to the extracellular region of human VLDL R, and 79% aa identical to the extracellular region of mouse LDL R. LDL R is constitutively expressed and binds apoB of LDL and apoE of VLDL. It is responsible for clearing 70% of plasma LDL in liver. Mutations in the LDL R gene cause the autosomal dominant disorder, familial hypercholesterolemia.

Long Name
Low Density Lipoprotein Receptor
Entrez Gene IDs
3949 (Human); 16835 (Mouse); 300438 (Rat)
Alternate Names
FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor

Product Datasheets

Citations for Human LDL R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. 6-Gingerol Regulates Hepatic Cholesterol Metabolism by Up-regulation of LDLR and Cholesterol Efflux-Related Genes in HepG2 Cells
    Authors: X Li, J Guo, N Liang, X Jiang, Y Song, S Ou, Y Hu, R Jiao, W Bai
    Front Pharmacol, 2018;9(0):159.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Low-density lipoprotein (LDL)-dependent uptake of Gram-positive lipoteichoic acid and Gram-negative lipopolysaccharide occurs through LDL receptor
    Authors: PM Grin, DJ Dwivedi, KM Chathely, BL Trigatti, A Prat, NG Seidah, PC Liaw, AE Fox-Robich
    Sci Rep, 2018;8(1):10496.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. Applying antibody-sensitive hypervariable region 1-deleted hepatitis C virus to the study of escape pathways of neutralizing human monoclonal antibody AR5A
    Authors: R Velázquez-, M Law, J Bukh, J Prentoe
    PLoS Pathog, 2017;13(2):e1006214.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  4. Antisense oligonucleotides targeting translation inhibitory elements in 5' UTRs can selectively increase protein levels
    Authors: XH Liang, H Sun, W Shen, S Wang, J Yao, MT Migawa, HH Bui, SS Damle, S Riney, MJ Graham, RM Crooke, ST Crooke
    Nucleic Acids Res., 2017;45(16):9528-9546.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  5. Ruxolitinib and polycation combination treatment overcomes multiple mechanisms of resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus
    Authors: SA Felt, GN Droby, VZ Grdzelishv
    J. Virol., 2017;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  6. Identification of a Chrysanthemic Ester as an Apolipoprotein E Inducer in Astrocytes
    PLoS ONE, 2016;11(9):e0162384.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  7. Alirocumab, a Therapeutic Human Antibody to PCSK9, Does Not Affect CD81 Levels or Hepatitis C Virus Entry and Replication into Hepatocytes
    PLoS ONE, 2016;11(4):e0154498.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  8. A novel mechanism causing familial hypercholesterolemia: The proprotein convertase subtilisin/kexin type 9-resistant Arg410Ser LDL receptor mutation
    Authors: Delia Susan-Resi
    J. Biol. Chem, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lystates
    Applications: WB
  9. Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation
    Authors: Elodie Weider
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  10. Common Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Epitopes Mediate Multiple Routes for Internalization and Function.
    Authors: DeVay R, Yamamoto L, Shelton D, Liang H
    PLoS ONE, 2015;10(4):e0125127.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  11. Plasma Membrane Tetraspanin CD81 Complexes with Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and Low Density Lipoprotein Receptor (LDLR), and Its Levels Are Reduced by PCSK9.
    Authors: Le Q, Blanchet M, Seidah N, Labonte P
    J Biol Chem, 2015;290(38):23385-400.
    Species: Human
    Sample Types: Cell Lysates
    Applications: IP
  12. Differential complement activation pathways promote C3b deposition on native and acetylated LDL thereby inducing lipoprotein binding to the complement receptor 1.
    Authors: Klop B, van der Pol P, van Bruggen R, Wang Y, de Vries M, van Santen S, O'Flynn J, van de Geijn G, Njo T, Janssen H, de Man P, Jukema J, Rabelink T, Rensen P, van Kooten C, Cabezas M
    J Biol Chem, 2014;289(51):35421-30.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Blocking
  13. Hormonal modulators of glial ABCA1 and apoE levels.
    Authors: Fan, Jianjia, Shimizu, Yoko, Chan, Jeniffer, Wilkinson, Anna, Ito, Ayaka, Tontonoz, Peter, Dullaghan, Edie, Galea, Liisa A, Pfeifer, Tom, Wellington, Cheryl L
    J Lipid Res, 2013;54(11):3139-50.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  14. Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
    Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
    J Biol Chem, 2013;288(15):10805-18.
  15. The proprotein convertase PC7: unique zymogen activation and trafficking pathways.
    Authors: Rousselet E, Benjannet S, Hamelin J, Canuel M, Seidah NG
    J. Biol. Chem., 2011;286(4):2728-38.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  16. Dissection of the endogenous cellular pathways of PCSK9-induced low density lipoprotein receptor degradation: evidence for an intracellular route.
    Authors: Poirier S, Mayer G, Poupon V, McPherson PS, Desjardins R, Ly K, Asselin MC, Day R, Duclos FJ, Witmer M, Parker R, Prat A, Seidah NG
    J. Biol. Chem., 2009;284(42):28856-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human LDL R Antibody
By Anonymous on 04/21/2016
Application: Flow Sample Tested: HEK293 human embryonic kidney cell line Species: Human