Human Leptin Quantikine QuicKit ELISA

Catalog # Availability Size / Price Qty
QK398
Control Products Available
Human Leptin ELISA Standard Curve
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Product Details
Procedure
FAQs
Supplemental Products
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Human Leptin Quantikine QuicKit ELISA Summary

Assay Length
80 minutes
Sample Type & Volume Required Per Well
Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
1.03 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Leptin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® QuicKit™ Human Leptin Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human Leptin levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Leptin and antibodies raised against the recombinant protein. Results obtained using natural human Leptin showed linear curves that were parallel to the standard curves obtained using the QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human Leptin.

Precision

Intra-Assay Precision (Precision within an assay) <div>Two samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 20 20 10 10
Mean 134 709 130 709
Standard Deviation 2.84 15.8 11.9 19.7
CV% 2.1 2.2 9.2 2.8

Recovery

The recovery of human Leptin spiked to three levels in samples throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 89 80-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Leptin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Human Leptin ELISA Linearity Curve

Data Examples

Human Leptin ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Leptin/OB

Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.

Entrez Gene IDs:
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names:
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

Assay Procedure

These assays utilize an anti-tag coated microplate. Standards, samples, and controls are added to plate, followed by subsequent addition of an antibody cocktail. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.

QuicKit ELISA assay procedure

FAQs

No product specific FAQs exist for this product, however you may

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ELISA Controls

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