Human Leptin R Quantikine ELISA Kit

  (9 citations)     
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Product Details
Assay Procedure
Citations (9)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.128 ng/mL
  • Assay Range
    0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human Leptin sR
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC112 , Quantikine Immunoassay Control Set 804 for Human Leptin R - Please Inquire
Product Summary
The Quantikine Human Leptin sR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Leptin sR in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Leptin R/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Leptin sR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Leptin sR.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1.83 5.15 11.2 1.69 5.09 10.7
Standard Deviation 0.071 0.283 0.292 0.097 0.282 0.633
CV% 3.9 5.5 2.6 5.7 5.5 5.9

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 2.34 7.01 14.6 2.34 6.95 14.3
Standard Deviation 0.142 0.155 0.709 0.202 0.37 0.966
CV% 6.1 2.2 4.9 8.6 5.3 6.7

Recovery

The recovery of human Leptin sR spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 91-102
EDTA Plasma (n=4) 104 92-115
Heparin Plasma (n=4) 103 92-113
Serum (n=4) 106 95-114
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Leptin sR were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Leptin R [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Leptin R

Leptin receptor (OBR), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OBR, including a long form (OBRL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OBRS) lacking signal-transducing capabilities, have been identified.

  • Long Name:
    Leptin Receptor
  • Entrez Gene IDs:
    3953 (Human); 16847 (Mouse)
  • Alternate Names:
    B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well.
  13.   For Serum & Plasma Samples: Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    For Cell Culture Supernate Samples: Incubate at room temperature for 20 minutes on the benchtop. PROTECT FROM LIGHT.

  14. 50 µL Stop Solution
  15. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Sample Type
  1. Metabolic milieu associates with impaired skeletal characteristics in obesity
    Authors: HT Viljakaine, HA Koistinen, T Tervaharti, T Sorsa, S Andersson, O Mäkitie
    PLoS ONE, 2017;12(6):e0179660.
    Species: Human
    Sample Type: Serum
  2. Soluble leptin receptor and risk of gestational diabetes in a multi-ethnic population - a prospective cohort study
    J Clin Endocrinol Metab, 2016;0(0):jc20162583.
    Species: Human
    Sample Type: Serum
  3. Leptin, Leptin Soluble Receptor, and the Free Leptin Index following a Diet and Physical Activity Lifestyle Intervention in Obese Males and Females
    Authors: Jeffrey E Herrick
    J Obes, 2016;2016(0):8375828.
    Species: Human
    Sample Type: Serum
  4. Dietary patterns and cardiometabolic and endocrine plasma biomarkers in US women
    Authors: Hala B AlEssa
    Am. J. Clin. Nutr, 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  5. Leptin and soluble leptin receptor levels in plasma and risk of type 2 diabetes in U.S. women: a prospective study.
    Authors: Sun Q, van Dam RM, Meigs JB, Franco OH, Mantzoros CS, Hu FB
    Diabetes, 2010;59(3):611-8.
    Species: Human
    Sample Type: Plasma
  6. Leptin exacerbates sepsis-mediated morbidity and mortality.
    Authors: Shapiro NI, Khankin EV, van Meurs M
    J. Immunol., 2010;185(1):517-24.
    Species: Human
    Sample Type: Plasma
  7. Differential bone metabolism between postmenopausal women with osteoarthritis and osteoporosis.
    Authors: Jiang LS, Zhang ZM, Jiang SD, Chen WH, Dai LY
    J. Bone Miner. Res., 2008;23(4):475-83.
    Species: Human
    Sample Type: Serum
  8. Absence of leptin resistance in platelets from morbidly obese individuals may contribute to the increased thrombosis risk in obesity.
    Authors: Dellas C, Schafer K, Rohm I, Lankeit M, Ellrott T, Faustin V, Riggert J, Hasenfuss G, Konstantinides S
    Thromb. Haemost., 2008;100(6):1123-9.
    Species: Human
    Sample Type: Plasma
  9. Leptin, blood pressure, and aerobic capacity in women.
    Authors: Sabatier MJ, McCully KK, Marinik EL, Schwark EH, Haddow S, Cortez-Cooper M, Bergeron MF, Sloan GJ, Cannon JG
    Am. J. Hypertens., 2008;21(11):1245-50.
    Species: Human
    Sample Type: Serum

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ELISA Controls
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Quantikine Immunoassay Control Set 804 for Human Leptin R

Ctrl QC112
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
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