Key Product Details

Species Reactivity

Human

Applications

Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all LILRA5/CD85f Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human LILRA5/CD85f
Gly42-Arg268
Accession # A6NI73

Specificity

Detects human LILRA5/CD85f in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human LILRA5/CD85f Antibody

Detection of LILRA5/CD85f antibody in Human peripheral blood monocytes antibody by Flow Cytometry.

Detection of LILRA5/CD85f in Human peripheral blood monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Sheep Anti-Human LILRA5/CD85f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6754, filled histogram) or control antibody (5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Applications for Human LILRA5/CD85f Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA binding assay, 1-7 μg/mL of this antibody will block 50% of the binding of 1 μg/mL of  Recombinant Human Angiopoietin-like 7 (Catalog # 914-AN) to immobilized Recombinant Human LILRA5/CD85f/LIR-9 (Catalog # 8956-T4) coated at 2 µg/mL (100 µL/well). At 15 µg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood monocytes

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRA5/CD85f

ILT11 (leukocyte immunoglobulin-like receptor 9; also CD85f, LIR-9 and LILRA5) is a 39-40 kDa Group 2 member of the LILR family of innate immune receptors. It is expressed on monocytes and perhaps neutrophils, and its activation results in the secretion of proinflammatory cytokines such as TNF-alpha and IL-1 beta. Mature human LIR-9 is a 258 amino acid (aa) type I transmembrane glycoprotein. It has a 227 aa extracellular domain (aa 42-268) that contains two C2-type Ig-like domains
(aa 51‑136 and 142-230), and a 10 aa cytoplasmic tail. LIR-9 has three potential splice forms. One is a 35 kDa soluble form of the molecule described above that shows a 27 aa substitution for aa 239-299. The other two splice forms are analogous to the above membrane and soluble forms, but demonstrate signal sequence cleavage further downstream after Ala51. Over aa 41‑268, human LIR-9 shares 58% aa identity with mouse LIR-9.

Long Name

Leukocyte Immunoglobulin-like Receptor Subfamily A Member 5

Alternate Names

CD85f, ILT11, LILRB7, LIR9

Entrez Gene IDs

353514 (Human); 232801 (Mouse); 691533 (Rat)

Gene Symbol

LILRA5

UniProt

A6NI73 (Human)

Additional LILRA5/CD85f Products

Product Documents for Human LILRA5/CD85f Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LILRA5/CD85f Antibody

For research use only

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