Human LMP7/PSMB8 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LMP7/PSMB8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human PSMB5 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human LMP7/PSMB8
    Thr73-Gln276
    Accession # P28062
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human LMP7/PSMB8 by Western Blot. Western blot shows lysates of human dendritic cells, Jurkat human acute T cell leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, Raji human Burkitt's lymphoma cell line, and HuT 78 human cutaneous T cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LMP7/PSMB8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7710) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LMP7/PSMB8 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LMP7/PSMB8

PSMB8 (Proteasome Subunit beta type-8; Also beta 5i, RING10/Y2 and LMP7) is a 23-24 kDa member of the peptidase T1B family of molecules. It is expressed both constitutively and inducibly by IFN-gamma in a wide variety of cells, including immature dendritic cells, preadipocytes, CD4+ T cells and monocytes. PSMB8 contributes to the 700 kDa, 20S proteasome catalytic complex, a dynamic intracellular structure that participates in ATP-dependent proteolytic activity. PSMB8 qualifies as a beta -type, i (immuno)-type proteasome, meaning it both plays a chymotrypsin-like role in the turnover of proteins, and is found in cytokine-responsive cells. The peptides generated through PSMB8 activity are used as immunogens by MHC-I molecules. PSMB8 activity is dependent upon the removal of the PSMB8 precursor prosequence, an action that exposes a critical internal Thr residue. Human PSMB8 is synthesized as a 28-29 kDa, 276 amino acid (aa) proprecursor. It contains a 72 aa autocleavable propeptide plus a 204 aa mature region. The mature region shows no identifiable standard structural motifs. There is one alternative splice form that shows a 45 aa substitution for aa 1-49. This isoform does not appear to participate in formation of a proteosome. Over aa 73-276, human PSMB8 shares 92% aa sequence identity with mouse PSMB8.

  • Long Name:
    Large Multifunctional Protease 7
  • Entrez Gene IDs:
    5696 (Human)
  • Alternate Names:
    ALDD; beta5i; D6S216E; EC 3.4.25.1; JMP; LMP7; LMP7MGC1491; Low molecular mass protein 7; low molecular weight protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; NKJO; protease component C13; proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalpeptidase 7); proteasome catalytic subunit 3i; Proteasome Component C13; proteasome subunit beta 5i; proteasome subunit beta type-8; Proteasome subunit beta-5i; Proteasome Subunit Y2; proteasome-related gene 7; PSMB5i; PSMB5iD6S216; PSMB8; Really interesting new gene 10 protein; RING10; RING10proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalprotease 7); Y2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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