Human LMP7/PSMB8 Antibody

Catalog # Availability Size / Price Qty
AF7710
AF7710-SP
Detection of Human LMP7/PSMB8 by Western Blot.
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Human LMP7/PSMB8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LMP7/PSMB8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human PSMB5 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LMP7/PSMB8
Thr73-Gln276
Accession # P28062
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human LMP7/PSMB8 by Western Blot. View Larger

Detection of Human LMP7/PSMB8 by Western Blot. Western blot shows lysates of human dendritic cells, Jurkat human acute T cell leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, Raji human Burkitt's lymphoma cell line, and HuT 78 human cutaneous T cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LMP7/PSMB8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7710) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LMP7/PSMB8 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMP7/PSMB8

PSMB8 (Proteasome Subunit beta type-8; Also beta 5i, RING10/Y2 and LMP7) is a 23-24 kDa member of the peptidase T1B family of molecules. It is expressed both constitutively and inducibly by IFN-gamma in a wide variety of cells, including immature dendritic cells, preadipocytes, CD4+ T cells and monocytes. PSMB8 contributes to the 700 kDa, 20S proteasome catalytic complex, a dynamic intracellular structure that participates in ATP-dependent proteolytic activity. PSMB8 qualifies as a beta -type, i (immuno)-type proteasome, meaning it both plays a chymotrypsin-like role in the turnover of proteins, and is found in cytokine-responsive cells. The peptides generated through PSMB8 activity are used as immunogens by MHC-I molecules. PSMB8 activity is dependent upon the removal of the PSMB8 precursor prosequence, an action that exposes a critical internal Thr residue. Human PSMB8 is synthesized as a 28-29 kDa, 276 amino acid (aa) proprecursor. It contains a 72 aa autocleavable propeptide plus a 204 aa mature region. The mature region shows no identifiable standard structural motifs. There is one alternative splice form that shows a 45 aa substitution for aa 1-49. This isoform does not appear to participate in formation of a proteosome. Over aa 73-276, human PSMB8 shares 92% aa sequence identity with mouse PSMB8.

Long Name
Large Multifunctional Protease 7
Entrez Gene IDs
5696 (Human)
Alternate Names
ALDD; beta5i; D6S216E; EC 3.4.25.1; JMP; LMP7; LMP7MGC1491; Low molecular mass protein 7; low molecular weight protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; NKJO; protease component C13; proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalpeptidase 7); proteasome catalytic subunit 3i; Proteasome Component C13; proteasome subunit beta 5i; proteasome subunit beta type-8; Proteasome subunit beta-5i; Proteasome Subunit Y2; proteasome-related gene 7; PSMB5i; PSMB5iD6S216; PSMB8; Really interesting new gene 10 protein; RING10; RING10proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalprotease 7); Y2

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