Detection of Human LNK/SH2B3 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, Ramos human Burkitt's lymphoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human LNK/SH2B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5888) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LNK/SH2B3 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LNK (also SH2B adaptor protein 3) is a protein intermediary, or LiNK, between cell surface molecules (TPO R; EPO R; SCFR; TCR) and downstream signaling molecules such as PI3 K, Grb2 and Jak2. Although its predicted MW is 63 kDa, it runs anomalously at 75 kDa in SDS‑PAGE. LNK is found in hematopoietic cells, and serves to limit responsiveness to growth factor stimulation. Human LNK is 575 amino acids (aa) in length. It contains an N-terminal Phe-zipper segment that likely serves as a dimerization domain (aa 24‑81), a PH domain that may interact with membrane lipids (aa 197‑305), and an SH2 domain that binds phosphoTyr (aa 364‑462).
Lymphocyte Adapter Protein
Entrez Gene IDs:
10019 (Human); 16923 (Mouse)
IDDM20; LNKSH2B adapter protein 3; Lymphocyte adapter protein; Lymphocyte-specific adapter protein Lnk; SH2B adaptor protein 3; SH2B3; Signal transduction protein Lnk
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Showing Results 1 - 1 of 1
LNK (SH2B3): paradoxical effects in ovarian cancer. Authors: Ding, L-W, Sun, Q-Y, Lin, D-C, Chien, W, Hattori, N, Dong, X-M, Gery, S, Garg, M, Doan, N B, Said, J W, Xiao, J-F, Yang, H, Liu, L-Z, Meng, X, Huang, R Y-J, Tang, K, Koeffler, H P Oncogene, 2015;34(11):1463-74. Species: Human Sample Type: Whole Tissue Application: IHC
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