Human LRMP Antibody

Catalog # Availability Size / Price Qty
AF6869
AF6869-SP
Detection of Human LRMP by Western Blot.
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Human LRMP Antibody Summary

Species Reactivity
Human
Specificity
Detects human LRMP in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human LRMP
Gln143-Pro292
Accession # AAI26418
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human LRMP by Western Blot. View Larger

Detection of Human LRMP by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LRMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6869) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRMP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRMP

LRMP (Lymphoid restricted membrane protein; also JAW1) is a 74-76 kDa protein that is associated with the ER. In contrast to its name, LRMP expression is not restricted to lymphoid cell types. Cells known to express LRMP include stomach chief cells, seminal vesicle cuboidal epithelium, neurons, germinal center B cells, cortical thymocytes, and cells of the taste buds. LRMP is suggested to participate in intracellular peptide transport. It is also reported to physically interact with IP3RP. Human LRMP is a 56 kDa (predicted), single-pass transmembrane (TM) protein that is 499 amino acids (aa) in length. It contains a 439 aa cytoplasmic region (aa 1-439), a 21 aa TM segment (aa 440-460) and a 39 aa luminal domain (aa 461-499) that is cleaved after Ala469 to generate a type IV TM protein. LRMP runs anomalously in SDS-Page, and the 75 kDa form may be linked to an isoform that utilizes a Leu start site 56 aa upstream of the start site for the 499 aa isoform. In this regard, a human isoform has been reported that is 62 kDa in size. Mature mouse LRMP is 509 aa in length (after cleavage) and runs at 65 kDa in SDS-PAGE. Over aa 143-292, human LRMP shares 93% aa identity with mouse LRMP.

Long Name
Lymphoid-restricted Membrane Protein
Entrez Gene IDs
4033 (Human); 16970 (Mouse); 500361 (Rat)
Alternate Names
Jaw1; JAW1Protein Jaw1; LRMP; lymphoid-restricted membrane protein

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