hh Human LRMP Antibody AF6869: R&D Systems

Human LRMP Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human LRMP in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human LRMP
    Gln143-Pro292
    Accession # AAI26418
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human LRMP by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LRMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6869) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRMP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LRMP

LRMP (Lymphoid restricted membrane protein; also JAW1) is a 74-76 kDa protein that is associated with the ER. In contrast to its name, LRMP expression is not restricted to lymphoid cell types. Cells known to express LRMP include stomach chief cells, seminal vesicle cuboidal epithelium, neurons, germinal center B cells, cortical thymocytes, and cells of the taste buds. LRMP is suggested to participate in intracellular peptide transport. It is also reported to physically interact with IP3RP. Human LRMP is a 56 kDa (predicted), single-pass transmembrane (TM) protein that is 499 amino acids (aa) in length. It contains a 439 aa cytoplasmic region (aa 1-439), a 21 aa TM segment (aa 440-460) and a 39 aa luminal domain (aa 461-499) that is cleaved after Ala469 to generate a type IV TM protein. LRMP runs anomalously in SDS-Page, and the 75 kDa form may be linked to an isoform that utilizes a Leu start site 56 aa upstream of the start site for the 499 aa isoform. In this regard, a human isoform has been reported that is 62 kDa in size. Mature mouse LRMP is 509 aa in length (after cleavage) and runs at 65 kDa in SDS-PAGE. Over aa 143-292, human LRMP shares 93% aa identity with mouse LRMP.

  • Long Name:
    Lymphoid-restricted Membrane Protein
  • Entrez Gene IDs:
    4033 (Human); 16970 (Mouse); 500361 (Rat)
  • Alternate Names:
    Jaw1; JAW1Protein Jaw1; LRMP; lymphoid-restricted membrane protein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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