Human Lymphotoxin-alpha /TNF-beta Antibody

MAB621 has been discontinued and is replaced by MAB621R.
  (1 citations)     
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Citations (1)
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  • Species Reactivity
    Human
  • Specificity
    Detects human Lymphotoxin‑ alpha /TNF‑ beta in ELISAs. In sandwich immunoassays, approximately 10% cross‑reactivity with recombinant mouse TNF‑ beta is observed and no cross-reactivity with bovine FGF acidic, recombinant human (rh) GM‑CSF, rhIL‑1 alpha, rhIL‑1 beta, rhIL‑2, rhIL‑3, rhIL‑4, rhIL‑6, rhIL‑8, rhTNF‑ alpha, rhPDGF, rpPDGF, rhTGF‑ beta 1, recombinant porcine (rp) TGF‑ beta 1, rpTGF‑ beta 1.2, or rpTGF‑ beta 2 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 5807
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human Lymphotoxin‑ alpha /TNF‑ beta
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
    • Human Lymphotoxin-alpha /TNF-beta Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human Lymphotoxin‑ alpha /TNF‑ beta Antibody (Catalog # MAB621 )
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Lymphotoxin‑ alpha /TNF‑ beta Biotinylated Antibody (Catalog # BAF211 )
  • ELISA Standard
     
    Recombinant Human Lymphotoxin-alpha/TNF-beta Protein (Catalog # 211-TBB )
  • Neutralization
    Measured by its ability to neutralize Lymphotoxin‑ alpha /TNF‑ beta -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.03-0.09 µg/mL in the presence of 0.25 ng/mL Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta and actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Cytotoxicity Induced by Lymphotoxin‑ alpha /TNF‑ beta and Neutral­ization by Human Lymphotoxin‑ alpha /TNF‑ beta Antibody.

Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta (Catalog # 211-TBB) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human Lymphotoxin‑ alpha /TNF‑ beta (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Lymphotoxin‑ alpha /TNF‑ beta Monoclonal Antibody (Catalog # MAB621). The ND50 is typically 0.03-0.09 µg/mL in the presence of the metabolic inhibitor actinomycin D.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lymphotoxin-alpha/TNF-beta

Tumor necrosis factor beta (TNF-beta ), also known as lymphotoxin-alpha (LT-alpha ), and TNF-alpha, are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical, effects. Among these effects is the ability to kill certain tumor cells directly, from which the names tumor necrosis factor and lymphotoxin both derive. Mature TNF-beta /LT-alpha and TNF-alpha share approximately 35% protein sequence homology and the biologically active secreted forms of both proteins are homotrimers. Whereas TNF-alpha can exist as a type II membrane protein, TNF-beta /LT-alpha possesses a typical signal peptide sequence and is a secreted protein. It has been shown that TNF-beta /LT-alpha is also present on the cell surface of activated T, B and LAK cells as a heteromeric complex with LT-beta, a type II membrane protein that is another member of the TNF ligand family. The genes for TNF-alpha, TNF-beta /LT-alpha, and LT-beta are closely linked within the major histocompatibility complex.

TNF-beta /LT-alpha is expressed in activated T- and B-lymphocytes. In addition to its cytotoxic action on tumor cells, TNF-beta /LT-alpha has been shown to be a mediator of inflammation and immune function. Evidence is also accumulating that TNF-beta /LT-alpha and TNF-alpha are mediators in the pathogenesis of certain autoimmune diseases. TNF-beta /LT-alpha has also been shown to have a role in lymphoid organ development. Human and mouse TNF-beta /LT-alpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity.

  • Long Name:
    Tumor Necrosis Factor beta
  • Entrez Gene IDs:
    4049 (Human); 16992 (Mouse)
  • Alternate Names:
    LT; LTA; LT-alpha; lymphotoxin alpha (TNF superfamily, member 1); Lymphotoxin alpha; Lymphotoxinalpha; Lymphotoxin-alpha; tnfb; TNF-beta; TNFBlymphotoxin-alpha; TNFSF1; TNFSF1B; TNFSF1TNF-beta; tumor necrosis factor beta; Tumor necrosis factor ligand superfamily member 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. A comprehensive ex vivo functional analysis of human NKT cells reveals production of MIP1-alpha and MIP1-beta, a lack of IL-17, and a Th1-bias in males.
    Authors: Snyder-Cappione JE, Tincati C, Eccles-James IG, Cappione AJ, Ndhlovu LC, Koth LL, Nixon DF
    PLoS ONE, 2010;5(11):e15412.
    Species: Human
    Sample Type: Whole Cells
    Application: ELISpot Development

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