Human Lysyl Oxidase Homolog 2/LOXL2 Antibody

• Species Reactivity
  Human
• Specificity
  Detects human Lysyl Oxidase Homolog 2/LOXL2 in direct ELISAs and Western blots.
  
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant human Lysyl Oxidase Homolog 2/LOXL2
  Gln26-Gln774
  Accession # AAH00594
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  1 µg/mL
  See below
• Simple Western
  10 µg/mL
  See below
• Immunoprecipitation
  25 µg/mL
  Conditioned cell culture medium spiked with Recombinant Human Lysyl Oxidase Homolog 2/LOXL2 (Catalog # 2639-AO), see our available Western blot detection antibodies

Western Blot click image to view larger

Detection of Human Lysyl Oxidase Homolog 2/LOXL2 by Western Blot. Western blot shows lysates of HEC‑1‑B human endometrial adenocarcinoma cell line and U‑87 MG human glioblastoma/astrocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Lysyl Oxidase Homolog 2/LOXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2639) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lysyl Oxidase Homolog 2/LOXL2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western click image to view larger

Detection of Human Lysyl Oxidase Homolog 2/LOXL2 by Simple WesternTM. Simple Western lane view shows lysates of U‑87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lysyl Oxidase Homolog 2/LOXL2 at approximately 109 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Lysyl Oxidase Homolog 2/LOXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2639) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Csiszar, H. (2001) Prog. Nucleic Acid Res. Mol. Biol. 70:1.
  2. Maki, J.M. and K.I. Kivirikko (2001) Biochem J. 355:381.
  3. Akiri, G. et al. (2003) Cancer Res. 63:1657.
  4. Hollosi, P. et al. (2009) Int. J. Cancer. 125:318.
  5. Peinado, H. et al. (2008) Cancer Res. 68:4541.
  6. Jourdan-Le Saux C. et al. (1999) J. Biol. Chem. 274:12939.

• Entrez Gene IDs:
  4017 (Human); 94352 (Mouse)

• Alternate Names:
  EC 1.4.3; EC 1.4.3.-; LOL2; LOR2; LOXL2; Lysyl Oxidase Homolog 2; lysyl oxidase related 2; lysyl oxidase-like 2; Lysyl oxidase-like protein 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14; WS9-14

FAQs

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