Human LYVE-1 Antibody

Catalog # Availability Size / Price Qty
AF2089
AF2089-SP
LYVE‑1 in Human Tonsil.
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Product Details
Citations (13)
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Human LYVE-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 35% cross-reactivity with recombinant mouse LYVE-1 and less than 1% cross-reactivity with recombinant human CD44 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LYVE-1
Ser24-Thr238
Accession # Q9Y5Y7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.30 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25-1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.4 - 2 µg/mL of this antibody will block 50% of the binding of 250 ng/mL of biotinylated Hyaluronan to immobilized Recombinant Human LYVE-1 (Catalog # 2089-LY) coated at 5 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry LYVE‑1 in Human Tonsil. View Larger

LYVE‑1 in Human Tonsil. LYVE‑1 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot Detection of Human LYVE‑1 by Western Blot. View Larger

Detection of Human LYVE‑1 by Western Blot. Western blot shows lysates of human liver and spleen tissue. PVDF membrane was probed with 0.25-1 µg/mL of Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for LYVE‑1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D‑glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).

References
  1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
  2. Evered, D and J. Whelan (1989) Ciba Found. Symp. 143:1.
  3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
  4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
  5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
  6. Jackson, D.J. et al. (2001)Trends Immunol. 22:317.
  7. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
  8. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
  9. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
  10. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.
Long Name
Lymphatic Vessel Endothelial Hyaluronan Receptor 1
Entrez Gene IDs
10894 (Human); 114332 (Mouse); 293186 (Rat)
Alternate Names
cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; LYVE-1XLKD1; XLKD1

Product Datasheets

Citations for Human LYVE-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Epithelial Membrane Protein-2 (EMP2) Antibody Blockade Reduces Corneal Neovascularization in an In Vivo Model
    Authors: MM Sun, AM Chan, SM Law, S Duarte, D Diaz-Aguil, M Wadehra, LK Gordon
    Invest. Ophthalmol. Vis. Sci., 2019;60(1):245-254.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: IHC
  2. Silencing of Discoidin Domain Receptor-1 (DDR1) Concurrently Inhibits Multiple Steps of Metastasis Cascade in Gastric Cancer
    Authors: R Yuge, Y Kitadai, H Takigawa, T Naito, N Oue, W Yasui, S Tanaka, K Chayama
    Transl Oncol, 2018;11(3):575-584.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Induced dural lymphangiogenesis facilities soluble amyloid-beta clearance from brain in a transgenic mouse model of Alzheimer's disease
    Authors: YR Wen, JH Yang, X Wang, ZB Yao
    Neural Regen Res, 2018;13(4):709-716.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Lymph nodes are sites of prolonged bacterial persistence during Mycobacterium tuberculosis infection in macaques
    Authors: SKC Ganchua, AM Cadena, P Maiello, HP Gideon, AJ Myers, BF Junecko, EC Klein, PL Lin, JT Mattila, JL Flynn
    PLoS Pathog., 2018;14(11):e1007337.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  5. VIPAR, a quantitative approach to 3D histopathology applied to lymphatic malformations
    Authors: R Hägerling, D Drees, A Scherzinge, C Dierkes, S Martin-Alm, S Butz, K Gordon, M Schäfers, K Hinrichs, P Ostergaard, D Vestweber, T Goerge, S Mansour, X Jiang, PS Mortimer, F Kiefer
    JCI Insight, 2017;2(16):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4
    Authors: LD Volk-Drape, KL Hall, AC Wilber, S Ran
    PLoS ONE, 2017;12(6):e0179257.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. TGF-?1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT)
    PLoS ONE, 2016;11(9):e0162221.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
    Authors: Riordan D, Varma S, West R, Brown P
    PLoS ONE, 2015;10(7):e0128975.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Adrenomedullin blockade suppresses growth of human hormone-independent prostate tumor xenograft in mice.
    Authors: Berenguer-Daize C, Boudouresque F, Bastide C, Tounsi A, Benyahia Z, Acunzo J, Dussault N, Delfino C, Baeza N, Daniel L, Cayol M, Rossi D, El Battari A, Bertin D, Mabrouk K, Martin P, Ouafik L
    Clin Cancer Res, 2013;19(22):6138-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. An in vivo platform for tumor biomarker assessment.
    Authors: Servais EL, Suzuki K, Colovos C
    PLoS ONE, 2011;6(10):e26722.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Effects of acute exercise, exercise training, and diabetes on the expression of lymphangiogenic growth factors and lymphatic vessels in skeletal muscle.
    Authors: Kivela R, Silvennoinen M, Lehti M, Kainulainen H, Vihko V
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(4):H2573-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
    Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
    Arthritis Rheum., 2007;56(6):1817-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Notch alters VEGF responsiveness in human and murine endothelial cells by direct regulation of VEGFR-3 expression.
    Authors: Shawber CJ, Funahashi Y, Francisco E, Vorontchikhina M, Kitamura Y, Stowell SA, Borisenko V, Feirt N, Podgrabinska S, Shiraishi K, Chawengsaksophak K, Rossant J, Accili D, Skobe M, Kitajewski J
    J. Clin. Invest., 2007;117(11):3369-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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