Human M-CSF Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2161G-100UG
Detection  of M‑CSF in Human Macrophages by Flow Cytometry.
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Human M-CSF Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human M-CSF.
Source
Monoclonal Mouse IgG2A Clone # 26786
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human M-CSF
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of M‑CSF in Human Macrophages by Flow Cytometry. View Larger

Detection of M‑CSF in Human Macrophages by Flow Cytometry. Human macrophages treated with LPS and Recombinant Human TNF-alpha (Catalog # 210-TA) were stained with Mouse Anti-Human M‑CSF Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC2161G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: M-CSF

M-CSF, also known as CSF-1, is produced by a variety of cell types in either a membrane-anchored or secreted soluble form. It is a glycosylated, disulfide-linked homodimer that binds to the M-CSF receptor expressed by cells of the monocyte-macrophage lineage.

Long Name
Macrophage Colony Stimulating Factor
Entrez Gene IDs
1435 (Human); 12977 (Mouse)
Alternate Names
colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930

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