Detection of M‑CSF in Human Macrophages by Flow Cytometry.
Human macrophages treated with LPS and Recombinant Human TNF-alpha (Catalog # 210-TA) were stained with Mouse Anti-Human M‑CSF Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC2161G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
M-CSF, also known as CSF-1, is produced by a variety of cell types in either a membrane-anchored or secreted soluble form. It is a glycosylated, disulfide-linked homodimer that binds to the M-CSF receptor expressed by cells of the monocyte-macrophage lineage.
Have you used Human M-CSF Alexa Fluor® 488-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.