Intracellular Staining by Flow Cytometry
|Detection of M‑CSF in Human Macrophages by Flow Cytometry. Human macrophages treated with LPS and Recombinant Human TNF-alpha (Catalog # 210-TA) were stained with Mouse Anti-Human M‑CSF PE‑conjugated Monoclonal Antibody (Catalog # IC2161P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
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