Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 26786
View all M-CSF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human M-CSF

Specificity

Detects human M-CSF in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human M‑CSF PE‑conjugated Antibody

Detection of M-CSF antibody in Human Macrophages antibody by Flow Cytometry.

Detection of M‑CSF in Human Macrophages by Flow Cytometry.

Human macrophages treated with LPS and Recombinant Human TNF-a (210-TA) were stained with Mouse Anti-Human M-CSF PE-conjugated Monoclonal Antibody (Catalog # IC2161P, filled histogram) or isotype control antibody (IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of M‑CSF in MG-63 cells by Flow Cytometry.

MG-63 cells were stained with Mouse Anti-Human M‑CSF PE‑conjugated Monoclonal Antibody (Catalog # IC2161P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human M‑CSF PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µL/106 cells
Sample: Human macrophages treated with LPS and Recombinant Human TNF-alpha (Catalog # 210-TA) fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and MG‑63 human osteosarcoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: M-CSF

M-CSF, also known as CSF-1, is produced by a variety of cell types in either a membrane-anchored or secreted soluble form. It is a glycosylated, disulfide-linked homodimer that binds to the M-CSF receptor expressed by cells of the monocyte-macrophage lineage.

Long Name

Macrophage Colony Stimulating Factor

Alternate Names

CSF-1, CSF1, Lanimostim, MCSF

Entrez Gene IDs

1435 (Human); 12977 (Mouse)

Gene Symbol

CSF1

Additional M-CSF Products

Product Documents for Human M‑CSF PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human M‑CSF PE‑conjugated Antibody

For research use only

Citations for Human M‑CSF PE‑conjugated Antibody

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