Human M-CSF Quantikine ELISA Kit

  (21 citations)     
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Citations (21)
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  • Assay Length
    4.5 hours
  • Specificity
    Natural and recombinant human M-CSF
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC21 , Quantikine Immunoassay Control Group 4 - Please Inquire
Product Summary
The Quantikine Human M-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human M-CSF in cell culture supernates, serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human M-CSF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human M-CSF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human M-CSF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Saliva, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 248 751 1503 280 827 1620
Standard Deviation 19.4 16.1 49 30.2 76 87.7
CV% 7.8 2.1 3.3 10.7 9.2 5.4

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 357 951 1767 343 1019 1956
Standard Deviation 17.4 24.9 49.4 43.4 84.1 88.8
CV% 4.9 2.6 2.8 12.6 8.3 4.5

Recovery

The recovery of M-CSF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 90-107
EDTA Plasma (n=4) 100 93-106
Heparin Plasma (n=4) 99 93-106
Serum (n=4) 101 96-106
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of M-CSF were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 M-CSF [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: M-CSF
M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.
  • Long Name:
    Macrophage Colony Stimulating Factor
  • Entrez Gene IDs:
    1435 (Human); 12977 (Mouse)
  • Alternate Names:
    colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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Species
Sample Type
  1. Effects of loxoprofen on the apical root resorption during orthodontic tooth movement in rats
    Authors: T Yamamoto, M Kaku, H Sumi, Y Yashima, J Izumino, K Tanimoto
    PLoS ONE, 2018;13(4):e0194453.
    Species: Human
    Sample Type: Cell Culture Supernates
  2. Serum bone-turnover biomarkers are associated with the occurrence of peripheral and axial arthritis in psoriatic disease: a prospective cross-sectional comparative study
    Authors: DR Jadon, R Sengupta, A Nightingal, H Lu, J Dunphy, A Green, JT Elder, RP Nair, E Korendowyc, MA Lindsay, NJ McHugh
    Arthritis Res. Ther., 2017;19(1):210.
    Species: Human
    Sample Type: Serum
  3. New Markers of Inflammation and Tubular Damage in Children with Chronic Kidney Disease
    Authors: K Musia?, A Bargenda, D Dro?d?, D Zwoli?ska
    Dis. Markers, 2017;2017(0):9389432.
    Species: Human
    Sample Type: Urine
  4. Effect of low-level laser therapy on bisphosphonate-treated osteoblasts
    Authors: Sang-Hun Shin
    Maxillofac Plast Reconstr Surg, 2016;38(1):48.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. Urokinase receptor mediates osteoclastogenesis via M-CSF release from osteoblasts and the c-Fms/PI3K/Akt/NF-kappaB pathway in osteoclasts.
    Authors: Kalbasi Anaraki P, Patecki M, Tkachuk S, Kiyan Y, Haller H, Dumler I
    J Bone Miner Res, 2015;30(2):379-88.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Induced osteoclastogenesis by fluoroquinolones in unstimulated and stimulated human osteoclast precursor cells.
    Authors: Costa-Rodrigues J, Martins EG, Fernandes MH
    Bone, 2012;51(1):17-27.
    Species: Human
    Sample Type: Cell Culture Supernates
  7. Engulfment of hematopoietic stem cells caused by down-regulation of CD47 is critical in the pathogenesis of hemophagocytic lymphohistiocytosis.
    Authors: Kuriyama T, Takenaka K, Kohno K, Yamauchi T, Daitoku S, Yoshimoto G, Kikushige Y, Kishimoto J, Abe Y, Harada N, Miyamoto T, Iwasaki H, Teshima T, Akashi K
    Blood, 2012;120(19):4058-67.
    Species: Human
    Sample Type: Serum
  8. Hypoxia potentiates glioma-mediated immunosuppression.
    Authors: Wei J, Wu A, Kong LY, Wang Y, Fuller G, Fokt I, Melillo G, Priebe W, Heimberger AB
    PLoS ONE, 2012;6(1):e16195.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. Whole-Body UVB Irradiation during Allogeneic Hematopoietic Cell Transplantation Is Safe and Decreases Acute Graft-versus-Host Disease.
    Authors: Kreutz M, Karrer S, Hoffmann P
    J. Invest. Dermatol., 2012;132(1):179-87.
    Species: Human
    Sample Type: Serum
  10. Circulating level of macrophage colony-stimulating factor can be predictive for human in vitro fertilization outcome.
    Authors: Salmassi A, Mettler L, Jonat W
    Fertil. Steril., 2010;93(1):116-23.
    Species: Human
    Sample Type: Serum
  11. EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation.
    Authors: Seizer P, Schonberger T, Schott M, Lang MR, Langer HF, Bigalke B, Kramer BF, Borst O, Daub K, Heidenreich O, Schmidt R, Lindemann S, Herouy Y, Gawaz M, May AE
    Atherosclerosis, 2010;209(1):51-7.
    Species: Human
    Sample Type: Cell Culture Supernates
  12. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009;206(1):169-81.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Type: Cell Culture Supernates
  14. Innate immune responses to TREM-1 activation: overlap, divergence, and positive and negative cross-talk with bacterial lipopolysaccharide.
    Authors: Dower K, Ellis DK, Saraf K, Jelinsky SA, Lin LL
    J. Immunol., 2008;180(5):3520-34.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. Exogenous heat shock protein 27 uniquely blocks differentiation of monocytes to dendritic cells.
    Authors: Laudanski K, De A, Miller-Graziano C
    Eur. J. Immunol., 2007;37(10):2812-24.
    Species: Human
    Sample Type: Cell Culture Supernates
  16. Markers of immune activation and viral load in HIV-associated sensory neuropathy.
    Authors: Schifitto G, McDermott MP, McArthur JC, Marder K, Sacktor N, McClernon DR, Conant K, Cohen B, Epstein LG, Kieburtz K, Kieburtz K
    Neurology, 2005;64(5):842-8.
    Species: Human
    Sample Type: Plasma
  17. The cell-surface isoform of colony stimulating factor 1 (CSF1) restores but does not completely normalize fecundity in CSF1-deficient mice.
    Authors: Ovadia S, Insogna K, Yao GQ
    Biol. Reprod., 2005;74(2):331-6.
    Species: Human
    Sample Type: Tissue Homogenates
  18. Presence of dendritic cells, MCP-1, and activated microglia/macrophages in amyotrophic lateral sclerosis spinal cord tissue.
    Authors: Henkel JS, Engelhardt JI, Siklos L, Simpson EP, Kim SH, Pan T, Goodman JC, Siddique T, Beers DR, Appel SH
    Ann. Neurol., 2004;55(2):221-35.
    Species: Human
    Sample Type: CSF
  19. The effect of inflammatory cytokines on secretion of macrophage colony-stimulating factor and monocyte chemoattractant protein-1 in human granulosa cells.
    Authors: Kawano Y, Fukuda J, Itoh H, Takai N, Nasu K, Miyakawa I
    Am. J. Reprod. Immunol., 2004;52(2):124-8.
    Species: Human
    Sample Type: Follicular Fluid
  20. Distinct hematopoietic support by two human stromal cell lines.
    Authors: Loeuillet C, Bernard G, Remy-Martin J, Saas P, Herve P, Douay L, Chalmers D
    Exp. Hematol., 2001;29(6):736-45.
    Species: Human
    Sample Type: Cell Culture Supernates
  21. High macrophage-colony stimulating factor levels in synovial fluid of loose artificial hip joints.
    Authors: Takei I, Takagi M, Ida H, Ogino T, Santavirta S, Konttinen YT
    J. Rheumatol., 2000;27(4):894-9.
    Species: Human
    Sample Type: Synovial Fluid
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